Biomedical subjects

Grzegorz Szymczyk

Publications and source records attributed to Grzegorz Szymczyk.

5 recordsLinked to original sources

PubMed2004

[Decrease of paraoxonase activity in the first day after abdominal operations in women].

INTRODUCTION: Paraoxonase (PON1) is one of enzymes participating in antioxidative defence mechanism, which protects the organism from the results of action of reactive oxygen species. PON1 is an esterase contained in plasma high density lipoproteins (HDL) which protects HDL from peroxidation and plasma membranes from free radical injury. PON1 serum activity is diminished in some diseases (atherosclerosis, diabetes, coronary artery disease) and after cardiosurgery operations. But there is no information about changes in PON1 activity after abdominal operations. THE AIM: Of this study was the assessment of PON1 serum activity after the abdominal operations in women. MATERIALS AND METHODS: 40 women operated in the 1st Department of Gynaecology of Lublin Medical University were studied, including 34 patients after total abdominal hysterectomy with salpingo-oophorectomy and 6 patients after myomectomy or cyst enucleation. The blood to experiments was collected before operation, and 8 and 24 hours after. PON1 serum activity was assessed spectrophotometrically toward two synthetic substrates: paraoxon and phenyl acetate. RESULTS: PON1 activity toward paraoxon before operation was 146.8 +/- 17.3 U/ml, and after 8 and 24 hours after 107.6 +/- 11.9 U/ml and 119.9 +/- 16.9 U/ml, respectively (results statistically significant). PON1 activity toward phenyl acetate (arylesterase activity) before operation was 182.7 +/- 16.4 U/ml and did not significantly changed after the surgery. CONCLUSION: It is observed decreased PON1 serum activity in the early postoperative period after abdominal operations in women, what can be connected with the utilisation of PON1 in antioxidative defence mechanism.

Adult↗

PubMed2003

[Non-closure of the peritoneum after hysterectomy--postoperative clinical assessment].

Dynamic development in modern medicine permanently brings the modifications of operation techniques. Both closure and non-closure of the peritoneum after gynecologic operations has been discussed recently. According to clinical observations provided by many authors, the controversial problem of non-closure of the peritoneum is still under discussion. Thus we decided to assess the influence of closing or non-closing of the peritoneum on short-term postoperative morbidity. The patients operated because of uterine myomae were the participants of our study. Total abdominal hysterectomy with suturing or non-closing of visceral and parietal peritoneum was performed. Our study revealed that non-closing of the peritoneum did not increase the amount of postoperative complications and it seemed to be a safe procedure.

Adult↗

PubMed2003

[Assessment of serum lipid peroxide levels and antioxidant status in females who had undergone total abdominal hysterectomy without closing of the peritoneum].

INTRODUCTION: Reactive oxygen species (ROS) generated after the trauma caused by surgical intervention, have the capacity to react in an indiscriminate manner leading to damage to almost any cellular component (proteins, nucleid acids) and to lipid peroxidation. The assessment of ROS generation intensity process may help in understanding of molecular processes observed in the intracellular environment after operations. The evaluation of antioxidative level may give an answer about the efficiency of antioxidative defence mechanism. The peritoneum is an organ of high metabolic activity, connected with ROS generation and participated in healing process. The modern opinions suggest closing of postoperative wound without closing of peritoneum. The aim of this study was assessment of antioxidant status and serum lipid peroxide levels in early postoperative period, in females who had undergone total abdominal hysterectomy without closing of peritoneum. MATERIALS AND METHODS: 30 women after total abdominal hysterectomy with salpingooophorectomy were studied, including 13 patients after peritoneal suturing (control group--C) and 17 women with nonclosed peritoneum (study group--S). The total serum antioxidant status (FRAP) was evaluated spectrophotometrically. The concentration of lipid peroxidation products was assessed in serum collected before operation and 8 and 24 hours after, as the concentration of lipid hydroxyperoxides (HPETE), malonyl dialdehyde (MDA) and 4-hydroxyalkenals (4-HDA). RESULTS: The antioxidative level before operation was 1147.09 +/- 93.6 microM/l in S group; 1022.04 +/- 115.4 microM/l in C group and did not changed significantly in early postoperative period. MDA + 4-HDA level before operation was 1.08 +/- 0.2 microM/l in S group and 1.06 +/- 0.2 microM/l in C group; HPETE level before operation was 4.58 +/- 0.1 microM/l in S group and 4.72 +/- 0.1 microM/l in C group, 8 and 24 hours after the operation MDA + 4-HDA level increased respectively to 1.34 +/- 0.2 microM/l (by 27%; p > 0.05) and 1.46 +/- 0.2 microM/l (37%) in S group and to 1.16 +/- 0.2 microM/l (by 8%) and 1.62 +/- 0.2 microM/l (by 52%; p < 0.05) in C group. HPETE level decreased to 4.56 +/- 0.1 microM/l (by 1%) and increased to 5.23 +/- 0.2 microM/l (by 14%) in S group and increased respectively to 6.0 +/- 0.2 microM/l (by 27%; p < 0.01) and to 6.43 +/- 0.2 microM/l (by 36%; p < 0.01) in C group. CONCLUSIONS: The concentration of lipid peroxidation products after the operation was lower in study group, where peritoneum was left without closing. Total antioxidative level was the same in both groups. Obtained results suggest smaller ROS generation in study group.

Biomarkers↗

PubMed2002

[The dually perfused human placenta by Schneider method modified by Miller. I. Changes of arterial and venous pH in material and fetal blood].

The study was carried out on 7 human placental cotyledons. Placental perfusions performed by Schneider method modified by Miller were conducted for 180 min. Assessment of arterial and venous pH in maternal and fetal blood was performed. There were no statistically significant differences between obtained parameters throughout the experiment. The results of this study point to the conclusion that the method can be used for assessment of other perfusion liquid parameters and introduced to subsequent experiments.

Female↗

PubMed2002

[The dually perfused human placenta by Schneider method modified Miller. II. Changes of arterial and venous fetal blood pressure].

The study was carried out on 7 human placental cotyledons. Placental perfusions performed by Schneider method modified by Miller were conducted for 180 min. assessment of arterial and venous pressure in fetal blood was performed. There were no statistically significant differences between obtained values of perfusion pressure obtained the experiment. The range of vessels perfusion resistance was stable during the experiment. The results ot this study lead to conclusion that the method used can be valuable for assessment of other perfusion liquid parameters and introduced to subsequent experiments.

Female↗