Biomedical subjects

Gang Ma

Publications and source records attributed to Gang Ma.

22 records · Page 2Linked to original sources

PubMed2002

Transcriptional activity of interferon regulatory factor (IRF)-3 depends on multiple protein-protein interactions.

Virus infection results in the activation of a set of cellular genes involved in host antiviral defense. IRF-3 has been identified as a critical transcription factor in this process. The activation mechanism of IRF-3 is not fully elucidated, yet it involves a conformational change triggered by the virus-dependent phosphorylation of its C-terminus. This conformational change leads to nuclear accumulation, DNA binding and transcriptional transactivation. Here we show that two distinct sets of Ser/Thr residues of IRF-3, on phosphorylation, synergize functionally to achieve maximal activation. Remarkably, we find that activated IRF-3 lacks transcriptional activity, but activates transcription entirely through the recruitment of the p300/CBP coactivators. Moreover, we show that two separate domains of IRF-3 interact with several distinct regions of p300/CBP. Interference with any of these interactions leads to a complete loss of transcriptional activity, suggesting that a bivalent interaction is essential for coactivator recruitment by IRF-3.

Amino Acid Sequence↗

PubMed2002

[Prognostic factor analysis of pneumonectomy for non-small cell lung cancer].

OBJECTIVES: To identify predictors of survival following pneumonectomy for non-small cell lung cancer (NSCLC) and provide evidence for the revision of patient selection criteria. METHODS: 81 cases of pneumonectomy for NSCLC from January 1990 to May 1996 at our hospital were reviewed retrospectively. There were 65 men (80.2%) and 16 women (19.8%), with a mean age 53.4 +/- 9.4 years (range 20 - 68 years). Predominant histological types included squamous cell carcinoma (54.3%), adenocarcinoma (24.7%), and squamoadenocarcinoma (17.3%). After follow-up for more than 5 years, data were examined using the chi-square test, Kaplan-Meier method, and Cox-mantel test. The possible factors affecting survival were tested with univariate and multivariate analysis. RESULTS: The 5-year survival of N(0), N(1) and N(2) disease of NSCLC following pneumonectomy was (20.8 +/- 9.9)%, (15.4 +/- 10.0)% and (4.0 +/- 2.8)%, respectively. There was no perioperative death. The operative complications morbidity was 22.2%. Factors adversely affecting survival with univariate analysis included age over 60 years for right pneumonectomy, cardiopulmonary complications, adenocarcinoma, peripheral location, tumor greatest dimension more than 10 cm, chest wall involvement and N(2) disease. Factors adversely affecting survival with multivariate analysis included cardiopulmonary complications, greatest tumor dimension more than 10 cm, chest wall involvement and N(2) disease. CONCLUSIONS: Pneumonectomy provides survival benefit with a high operative complications morbidity. Old age (>/= 60 years) for right pneumonectomy, cardiopulmonary complications, adenocarcinoma, and N(2) disease may be negative prognostic factors of long-term survival. Patient selection should be based on cardiopulmonary evaluation and the stage of disease.

Adult↗

PubMed2002

[Detection of telomerase hTERT gene expression of exfoliated cell in broncho-alveolar lavage fluid].

BACKGROUND & OBJECTIVES: The patients with lung carcinoma usually have high telomerase activity, the pseudopositive result is happen frequently when the test is done with traditional method. Telomerase activity is more strongly correlated with hTERT mRNA. This study was designed to detect the expression of telomerase hTERT gene in broncho-alveolar lavage fluid of the patients with lung carcinomas by relative quantitative RT-PCR method, to afford a useful tool for early and differentiate diagnosis of lung cancer. METHOD: The exfoliated cells in broncho-alveolar lavage fluid from 20 human suspicion lung carcinomas and 10 health persons were analyzed for hTERT expression by relative quantitative RT-PCR method, in the same time a part of exfoliated cells were detected with pathologic method. After operation, pathologic detection was performed for all samples. RESULTS: Of 20 human suspicion lung carcinomas and, 19 patients were lung cancer, 1 patient was inflammatory pseudotumor in pathological diagnosis after operation. The relative expression of telomerase hTERT gene in 16 cases of 19 patients with lung carcinoma were detected with the relative quantitative RT-PCR, the positive rate is 84.2% (16/19), the expressions of telomerase hTERT gene were at various quantities, average levels is 0.42. The positive rate in exfoliated cells detected with pathologic method were 57.9% (11/19). There was a statistical difference between them(P < 0.01). Ten normal persons were hTERT gene expression negative. CONCLUSIONS: Relative quantitative RT-PCR were more sensitive than cell morphologic method in detecting telomerase hTERT gene expression in broncho-alveolar lavage fluid of patients with lung carcinomas. Detecting quantitatively the levels of telomerase hTERT gene expression in broncho-alveolar lavage fluid will may be useful for early and differentiate diagnosis of lung carcinomas, especially for peripheral lung carcinomas.

Adult↗

PubMedDate not supplied

New insights into lung surfactant monolayers using vibrational sum frequency generation spectroscopy.

At the air-water interface, interfacial molecular structure, intermolecular interactions, film relaxation and film respreading of model lung surfactant monolayers were studied using vibrational sum frequency generation (VSFG) spectroscopy combined with a Langmuir film balance. Chain-perdeuterated dipalmitoylphosphatidylcholine (DPPC-d62), palmitoyloleoyl-phosphatidylglycerol (POPG), palmitic acid (PA) and tripalmitin were investigated. In the DPPC-d62-PA binary monolayer, PA showed a condensing effect on the DPPC chains. On the contrary, in the DPPC-d62-POPG binary monolayer, POPG showed a fluidizing effect on the DPPC chains. In the ternary monolayer system of DPPC-d62-POPG-PA, the balance between the fluidizing and the condensing effect was also observed. In addition, the film relaxation behavior of DPPC-d62 and the enhanced film stability of DPPC-d62 caused by the addition of tripalmitin were observed. Real-time VSFG was also employed to study the respreading properties of a complex lung surfactant mixture containing DPPC-d62, POPG, PA and KL4 (a mimic of SP-B) peptide, which revealed DPPC enrichment after film compression.

1,2-Dipalmitoylphosphatidylcholine↗