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Biomedical subjects

G Zhong

Publications and source records attributed to G Zhong.

At least 55 records · Page 3Linked to original sources
PubMed1996

[Transplantation of pedicled pisiform bone to replace lunate bone with aseptic necrosis].

OBJECTIVE: To find out a new and effective method for the treatment of aseptic necrosis of lunate bone. METHODS: Blood supply and morphology of pisiform bone were investigated in 25 upper limbs and 57 sets of carpal bone from adult cadavers on the basis of anatomical study. The lunate bone was replaced with pisiform bone of pedicled blood vessel and tendon of musculus flexor earpiulnaris in 17 patients with aseptic necrosis of lunate bone (stage III). RESULTS: The patients were followed up for 18-46 months (average of 28 months). Complete relief of pain was obtained in all of the patients, and the range of motion of their wrists was improved. Grip strength was increased by 48.2%. Radiograph showed normal location of transferred pisiform bone without osteosclerosis and atrophy in 16 patients. Atrophy of pisiform bone was found 2 years after operation in one patient. In the 17 patients, 15 resumed their original jobs and 2 changed their jobs for other reasons. CONCLUSION: We consider that the carpal bone chain can be completely preserved by transplantation of pedicled pisiform bone, which it is an effective method to treat aseptic necrosis stage III of lunate bone.

Adult↗
PubMed1996

[The hepatitis A virus isolated and adapted in human diploid fibroblast cells (KMB17)].

Three hepatitis A virus (HAV) strains (L8, H32 and H52) were isolated from stool specimens of hepatitis A patients during an epidemic in 1988 in Shanghai and were adapted to grow in human diploid fibroblast cell strain (KMB17). The strains were serially carried through 10 passages in KMB17. The infectious titrations of these virus strains cultured for 28 days at the 10th passage were high up to 7.0-7.75 log TCID50/ml. The maximum virus yields occured in the 28th day after infection. Immune electronic microscopic studies revealed that the viral particles were 27-30 nm in diameter. The strains were neutralized by specific anti-HAV serum. Special fluorescent stained particles were observed clearly in the infected cellular plasma by direct IF. The above data suggest that the three strains are HAV and may be useful for further study.

Cell Line↗
PubMed1995

[Technological studies of effects of inclusion on pericarpium Citri reticulatae volatile oil-beta-cyclodextrin].

The effects of inclusion on Pericarpium Citri Reticulatae volatile oil-beta-cyclodextrin were studied by comparing four methods saturation, grinding, liquid to liquid and liquid to gas. The utilization ratio of oil, the ratio of oil in inclusion complex and the recovery of inclusion complex were used as the criteria in evaluating the inclusion effectiveness. The results showed that the saturation method was the best.

Citrus↗
PubMed1995

[Extraction of intraocular magnetic foreign body using intraocular earth magnet].

PURPOSE: To determine the effects of extraction of intraocular magnetic foreign body with intraocular earth magnet (IOEM). METHOD: From 1992 to 1994, 22 cases with intraocular foreign body were treated with self-made intraocular earth magnet (IOEM): 2 patients with foreign body remained on lens, 7 on posterior segment of global wall, 12 lodged in the post pole of retina and 1 on the surface of optic disk. These patients were operated under microscope. The foreign bodies on the posterior segment were removed by using IOEM combined with pars plana vitrectomy. RESULT: The foreign bodies of these 22 patients were successfully removed with one operation respectively. The minimum volume of the foreign bodies was 0.5 x 0.5 x 0.5 mm3. The maximum volume of the foreign bodies was 5 x 2 x 1.5 mm3. The average maximum diameter of the foreign bodies was 2.47 +/- 1.2 mm. CONCLUSION: Intraocular earth magnet (IOEM) is a permanent magnetic instrument with moderate magnetic power. Direct observation and detachment of the foreign body with ocular tissue are necessary during the remowal of the forreign body. This instrument is suitable for intraocular microsurgery, can be handled easily, used for extraction of foreign bodies in anterior chamber, on turbid lens and in vitreous body, in removing foreign bodies in vitreous chamber with obvious injured retina and on posterior segment, the surface of retina, and can also be applied toextract foreign bodies on optic disk as well asforeign bodies partly oldged on global wall.

Adolescent↗
PubMed1994

Conformational mimicry of a chlamydial neutralization epitope on filamentous phage.

Conformational constraints were imposed on a peptide epitope from Chlamydia trachomatis to improve its ability to elicit antibodies that cross-react with native antigen. Appropriate constraints were discovered by a strategy that required no prior knowledge of the epitope's native conformation. First, we constructed a library of 3.2 x 10(5) peptides in which the epitope's contact residues were subject to random conformational constraints, each constrained peptide being fused genetically to the surface of a filamentous phage vector. Next, we selected phage displaying the most native-like peptides in the library by affinity purification with antibodies that bind the epitope only in its native conformation. Finally, we immunized mice with the selected phage and titered the resulting antisera against both whole cells and unconstrained peptide. The ratio of anti-cell titer to anti-peptide titer, which reflects the channeling of the antibody response to the native epitope, was up to five times higher for affinity-selected phage than for unselected peptide phage. In this case, therefore, "antigenic fitness," the ability of a peptide to bind antibodies specific for native epitope, correlated with "immunogenic fitness," its ability to elicit antibodies that are effective against the native antigen on an invading pathogen. If the correlation is general, surveying thousands or millions of peptides for antigenic fitness with phage display technology may be a simple but effective pre-screen for immunogenic fitness, which is costly to assess directly.

Amino Acid Sequence↗
PubMed1994

Antibody recognition of a neutralization epitope on the major outer membrane protein of Chlamydia trachomatis.

Two BALB/c mice were immunized with serovar C Chlamydia trachomatis elementary bodies, and 63 hybridomas producing monoclonal antibodies to C. trachomatis were recovered. Eight hybridomas which were specific for an identical peptide epitope (AGLQND) in serovar C major outer membrane protein variable domain I were identified. Detailed immunochemical study of the antigen-antibody interaction and genetic characterization of the antibody variable-region gene sequences showed that distinct B-cell clonal lineages were elicited by the epitope sequence. Since each antibody had a distinct pattern of fine specificity for recognition of the epitope and displayed different degrees of cross-reactivity with a related serovar (serovar A), we conclude that B-cell recognition of an immunodominant neutralization epitope can be pleiotropic. Differences in B-cell recognition of a neutralization epitope may delay the emergence by mutation of antigenic-drift variants of the C. trachomatis major outer membrane protein.

Amino Acid Sequence↗
PubMed1994

[A textual research on drinking disease and antidrinking remedies].

This subject is investigated through three aspects, viz., definition of drinking disease, its manifestations and treatment, and recipes and drug for antidrinking. The term "drinking disease" (Jiu Bing) was first recorded in Wang Shuhe's Canon of Pulsology, while abnormal drinking (Jiu Bo) was mentioned in Miraculous Pivot (Ling Shu). On Pathogenesis Manifestation of all diseases (Zhu Bing Yuan Hou Lun) gives the pathology and etiology. Great influence was exerted from Jin-yuan dynasties concerning its treatment. Zhang Jiebin expounded the comatous syndrome due to drinking (Jiu Jue). The earliest antidrinking drug was recorded in Shen Nong's Classic of Herbology. Through investigation, the commonly applied drug for relieving drunk include Flos Puerariae, Fructus Hovenariae, while the famous recipe for relieving drunk is Anti-tipsy Decoction of Flos Puerariae.

Alcoholism↗
PubMed1993

Immunogenicity evaluation of a lipidic amino acid-based synthetic peptide vaccine for Chlamydia trachomatis.

Lipidic amino acid-based synthetic peptides derived from the variable domains (VD) of Chlamydia trachomatis outer membrane protein 1 were evaluated as potential candidate sequences in a vaccine. A peptide sequence designated P2 from the VD IV of serovar B contained a B cell epitope capable of eliciting antibodies binding to serovar B elementary bodies (EB) and a T helper site capable of presentation by multiple H-2 alleles. Polymerization of the P2 into polylysine to form lipid core peptides (LCP) significantly enhanced immunogenicity compared with P2 monomer alone. The LCP system incorporates lipidic amino acids into the polylysine system and enhances lipophobicity and membrane binding effects of the peptide. A second peptide sequence derived from the VD I of serovar C was cosynthesized with P2 into lipidic polylysine LCP and was designated LCP-H1. Antibodies to this construct reacted at high titer with EB of the three major trachoma causing C. trachomatis serovars A, B, and C. LCP-H1 was immunogenic among four of five murine H-2 alleles. Pepscan analysis showed that the fine specificity of antibodies generated to LCP-H1 were directed to the predetermined neutralizing epitope sequences. An in vitro HAK cell neutralization assay showed that LCP-H1 elicited neutralizing antibodies to serovars A, B, and C, but these were of low titer. Because LCP-H1 antibodies bound to the peptide sequence with 10-100-fold higher titer than to EB, the low neutralization titers most likely result from conformational differences between the synthetic peptide and antigenic sites on the native organism. Modification of LCP-H1 to incorporate a predefined conformation may result in improved antigenic properties.

Adjuvants, Immunologic↗
PubMed1993

Continuous B-cell epitopes in Chlamydia trachomatis heat shock protein 60.

B-cell peptide epitopes in chlamydial heat shock protein 60 (hsp60) were elucidated with antisera from 13 rabbits immunized with Chlamydia trachomatis serovars B, C, and L2 and antisera from eight women with C. trachomatis-associated ectopic pregnancies. Thirteen major epitopes were identified with the human sera, 10 of which were also observed with rabbit antisera. Seven of the 13 epitopes recognized by human antisera exhibited cross-reactive antibody binding to homologous peptide sequences in human hsp60. Self-reactive B-cell immunity to hsp60 may contribute to chlamydial disease pathogenesis.

Amino Acid Sequence↗
PubMed1993

[Experimental research of acupuncture manipulation of reinforcing and reducing by lifting and thrusting needle on the animal].

The experiment were performed on model rabbits injected with large dosage of Dexamethasone (DXM) for 5 days. Reinforcing manipulation (RIM), reducing manipulation (RDM), and electric stimulation (ES) were respectively used to puncture Zusanli (ST 36) points for 5 days to observe the change on temperature, body weight, corticosterone assay, testosterone assay, and the amplitude of the plethysmogram. The result showed that there was a similar syndrome of the kidney deficiency after injecting DXM. The body weight was increased in the DXM control group, decreased in the RDM group and had no significant influence in the other groups. The body weights between the reinforcing manipulation and the reducing manipulation groups had obvious difference. But the other indices of observation had no significant influence among the different groups. So there wasn't significant difference on most of observed signs among the RIM group, the RDM group and ES group. The study of acupuncture manipulation will have yet to be further done.

Acupuncture Therapy↗
PubMed1993

[Clinical analysis of 160 cases with the magnetic foreign bodies in the posterior segment of the eye].

One hundred and sixty cases of magnetic foreign bodies in the posterior segment of the eye are reported. Most of the foreign bodies are small (61.2% are less than 2mm) and near the wall of the eye (73.7% are less than 2mm). 110 cases were successfully operated in conventional way and just 20 cases needed to have vitrectomy. The result of the operation depends on the damage of the eye, location of the foreign body and the complication of the operation. In 77.6% of the cases, the wound was on the cornea or limbus. That is one of the important factors to affect the patients' postoperation vision.

Cataract Extraction↗
PubMed1992

Antigenic analysis of the chlamydial 75-kilodalton protein.

Both B- and T-cell immunogenicity of a chlamydial 75-kDa protein was analyzed by using 131 partially overlapped decapeptide homologs of the 75-kDa protein from Chlamydia trachomatis serovar L2. Six rabbit antiserum specimens raised with serovars B, C, and L2 were used to assay the antibody reactivities of the decapeptides. Seventy-five of the 131 decapeptides were recognized by at least one antiserum specimen, and two peptides were found to be immunodominant and surface accessible on native organisms. The same set of decapeptides were cleaved from the pins and tested for their T-cell-stimulating activity in an in vitro proliferation assay. A single decapeptide was able to stimulate proliferation of chlamydial antigen-primed lymph node T cells from BALB/c mice.

Amino Acid Sequence↗
PubMed1992

Antibody responses to the chlamydial heat shock proteins hsp60 and hsp70 are H-2 linked.

The effects of both H-2 and non-H-2 genes on antibody responses to two Chlamydia trachomatis heat shock proteins (hsp60 and hsp70) were investigated. These chlamydial proteins are homologs of Escherichia coli GroEL (hsp60) and DnaK (hsp70) and are highly sequence conserved between bacterial and mammalian sources. Antibody responses among 17 different strains of mice immunized with C. trachomatis serovar B and serovar C elementary bodies were evaluated by immunoblot, radioimmunoprecipitation and enzyme-linked immunosorbent assay. Antibody responses to the two proteins displayed host genetic restriction. Of six distinctive H-2 haplotypes, only H-2d generated high antibody responses to hsp70. Five of the six H-2 haplotypes, i.e., H-2a, H-2d, H-2k, H-2q, and H-2s, produced high antibody responses to hsp60. Only the H-2b-bearing strain had low antibody responses to hsp60. By using congenic and H-2 recombinant strains, the genes responsible for regulating antibody responses to hsp70 and hsp60 were mapped to the K-IA region of the H-2 locus. In F1 hybrid crosses between high and low responders, high responses to hsp60 and hsp70 were dominant traits. Other genes outside the H-2 locus also influenced antibody responses to hsp60 and hsp70, since inbred strains of identical H-2 but different background genes displayed variable antibody responses to the proteins. The genetic control of murine immune responses to C. trachomatis hsp60, a putative chlamydial immunopathologic antigen, suggests that a similar genetic mechanism may also exist in humans, and this observation may help to explain the observed variability in the spectrum of chlamydial diseases seen in humans.

Animals↗
PubMed1992

[Vitrectomy combined with intravitreal injection of drugs for the management of advanced suppurative endophthalmitis].

Sixty cases (60 eyes) of advanced suppurative endophthalmitis treated by a combination of vitrectomy and intravitreal injection of drugs (antibiotics or/and corticosteroids) were investigated perspectively. Of the 60 cases, 43 were infected after trauma, 12 were infected after surgical procedures, while the others were of unknown causes. During the follow-up period of 1/2-5 years, 57 cases (95%) retained functioning eyes, the postoperative visual acuity of 34 eyes (57%) being 0.05 or better and the postoperative visual acuity of 17 eyes (28%) 0.4 to 1.0. The advantages of the therapeutic regimen are analyzed. We believe that it is the most effective therapy for suppurative endophthalmitis. The timing of the operation and the factors influencing the results are discussed. This report also presents our clinical experiences in the management of advanced suppurative endophthalmitis.

Adolescent↗
PubMed1992

[The effect of acupuncture on rabbits with fever caused by endotoxin].

Forty-eight rabbits were used to investigate the effect of puncturing GV14, LI11 on the change of temperature and the level of plasma endotoxin. The animals were divided into 5 groups: reinforcing manipulation, reducing manipulation, electroacupuncture, endotoxin-control, N.S.-control group. The result shows that there is no influence on the fever caused by endotoxin and the level of plasma endotoxin in the treatments by the different acupuncture techniques.

Acupuncture Therapy↗