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Biomedical subjects

G Zhang

Publications and source records attributed to G Zhang.

At least 325 records · Page 18Linked to original sources
PubMed1999

[The inhibitory effect of recombinant fibronectin polypeptide CH50 on the growth of B16 melanoma].

OBJECTIVE: To study the inhibitory effect of CH50, a recombinant polypeptide consisting of 2 binding domains of human fibronectin, on the growth of B16/F1 melanoma in mice. METHODS: Mice inoculated with B16/F1 cells were treated with CH50. The growth inhibitory effect on i.p. inoculated tumor was assessed by the number and size of tumor nodules in the peritoneal cavity, and on s.c. inoculated tumor by tumor size. RESULTS: For s.c. inoculated B16/F1, CH50 injected at the tumor site led to 50% inhibition of tumor growth while injection at a distant site was much less effective. In mice bearing i.p. inoculated tumor, the growth inhibition by CH50 on tumor nodules < 1 mm in diameter was 80% and 50+% on those > 1 mm. Synergistic therapeutic effect was obtained when CH50 used in combination with hydroxycamptothecine (HCPT). CONCLUSION: CH50 is effective in the treatment of B16/F1 melanoma. The effect can be enhanced when used in combination with HCPT.

Animals↗
PubMed1999

[Expression and significance of urokinase-type plasminogen activator in breast cancer].

OBJECTIVE: To study the expression and clinical significance of urokinase-type plasminogen activator (uPA) in breast cancer. METHODS: Applying streptavidin-biotin complex (SABC) immunohistochemical technique, expression of uPA was studied in 100 patients with primary breast cancer. RESULTS: There were 55 patients with high uPA expression, and 45 with low expression. There was significant correlation between uPA expression and TNM stage, lymph node status, tumor size. Neither age, menopausal status, nor ER status was significantly related with level of uPA expression Patients with high expression of uPA had significantly shorter disease-free survival period and overall survival than did those with low expression of uPA. Univariate analysis showed that uPA as a prognostic factor was of similar magnitude to lymph node status and TNM stage, but stronger than that of ER status and tumor size. uPA was an independent prognostic factor affecting disease-free survival and overall survival. CONCLUSION: uPA appears to be a strong and independent biologic marker for predicting prognosis of breast cancer.

Adult↗
PubMed1999

[Treatment of class II furcation involvement: guided tissue regeneration with atelocollagen membrane].

OBJECTIVE: To evaluate the effectiveness of guided tissue regeneration (GTR) on class II furcation involvement with absorbable atelocollagen membranes made in China. METHODS: GTR was performed on 26 sites with class II furcation defect in 17 patients. Clinical measurements and digital subtraction radiography were used to evaluate the effect. RESULTS: Clinical measurements showed significant attachment gain, significant decrease of probing depth (PD) and horizontal probing depth (HPD) at 3, 6, and 12 month after surgery. The mean reduction of HPD were 1.8 mm, 2.2 mm, 2.5 mm at 3 month (23 sites), 6 month (12 sites), and 12 month (6 sites) after surgery respectively. PD decreased by 1.22 mm, 1.56 mm and 1.75 mm. Attachment gained by 1.17 mm, 2.11 mm and 1.75 mm. Three sites showed complete defect fill during 3 to 12 months after surgery. Only 1 mm or less could be probed horizontally in another 4 furcation lesions during 3 to 12 months post-operation. The HPD of pre-operation in these 7 cases were 2.0 mm to 4.0 mm, and the bone defects observed during surgery were smaller in these cases than in other cases. The other 19 furcation involvement improved significantly. The result of digital subtraction radiography showed that increase of bone density was found at 2 months after surgery. The amount of bone gain increased with time. The areas of increased bone density were 0.3-25.7 mm2 in 13 teeth. CONCLUSION: The class II furcation involvement can be treated by GTR with absorbable atelocollagen membranes with good results.

Adult↗
PubMed1999

[Limited pleuro-pneumonectomy for bronchogenic carcinoma associated with cancerous pleural effusion].

OBJECTIVE: To evaluate the effects of limited pleuro-pneumonectomy for bronchogenic carcinoma associated with cancerous pleural effusion. METHODS: From January. 1994 to December 1997 we performed limited pulmonary lobectomy wedge resection of lung, resection of the metastases of the parietal pleura, visceral pleura, diaphragmatic pleura and pericardiac pleura, and electro incision of the micormetastases of the above position as well as cleaning the mediastinal lymph nodes in 14 cases. RESULTS: The 14 cases were followed-up to December 1998. Eight patients of them died, the other 6 patients survived with a survival period of 7 - 30 months (median survival period 16.5 months). No pleural effusions recurrence was seen. The longest survival period was 30 months. There were no operative mortality and no severe postoperative complication. Chest distress, dyspnea and pain of chest or abdomen were relieved. CONCLUSIONS: This simple method has such advantages as, less traumatic, rapid rehabilitation and effective control of pleural effusion. The postoperative survival period is longer than that of palliative operation and medical therapy. The pain caused by parietal pleural metastasis can be relieved.

Adenocarcinoma↗
PubMed1999

[Clinical protocols for the treatment of severe acute pancreatitis].

OBJECTIVE: To set up principles for the treatment of severe acute pancreatitis (SAP). METHODS: The therapeutic principles and results of SAP in three periods were analyzed. Period I (1980 - 1990) was characterized by early surgical intervention supplemented with integrated traditional and western medical therapy, period II (1991 - 1993) by integrated traditional and western medical therapy supplemented with surgery for early complications, and period III (1994 - 1997) by integrated traditional and western medical therapy supplemented with surgery for local infectious complications only. RESULTS: Because of the improvement of intensive care, the operation rates kept declining in the three periods. They were 77.59%, 54.55% and 29.55% with a mortality rate of 40.52%, 17.17% and 11.36%, respectively. CONCLUSIONS: Integrated traditional and western medicine has been effective in most SAP patients, and surgery is indicated only for late complications such as necrotizing infection. Early surgery should be restricted.

Adolescent↗
PubMed1999

[Cytokine assays for pterygium].

OBJECTIVE: To study the roles of tumor necrosis factor (TNF-alpha) and platelet-derived growth factor (PDGF) in pathogenesis of pterygium. METHODS: Radioimmunoassay and bioactivity assay were used to measure the levels of TNF-alpha and PDGF in tissue culture supernatant of pterygium specimens obtained from 30 patients. RESULTS: The levels of TNF-alpha and PDGF in tissue culture supernatant of pterygium were significantly higher than that of normal conjunctival specimens (t = 2.827, P < 0.01; t = 3.011, P < 0.01), and there was a significant difference between progressive and quiescent cases (t = 2.374, P < 0.05; t = 2.712, P < 0.05). CONCLUSIONS: The abnormal secretion of TNF-alpha and PDGF may be related to the pathogenesis of pterygium.

Adult↗
PubMed1999

[Experimental study on induction of apoptosis of leukemic cells by Boswellia carterii Birdw extractive].

The purpose of the study was to investigate the apoptosis of leukemic cells induced by Boswellia Carterii Birdw(BCB). The target leukemia cell line HL60 and bone marrow leukemic cells from 30 acute non-lymphocytic leukemic(ANLL) patients (3 M1 11 M2a 10 M3 1 M4a 5 M5b) were studied. Apoptosis was detected by morphological observation, DNA electrophoresis, percentage of DNA fragmentation test and flow cytometric cell cycle analysis. It is concluded that BCB can induce apoptosis in ANLL cells and HL60 cells.

Adolescent↗
PubMed1999

[Thrombosis induced by homocysteine does not depend on platelet aggregation or heparin cofactor's antithrombin activity].

OBJECTIVES: To partly elucidate the relationship between homocysteine(Hcy) and thrombosis, we observed Hcy's effects on platelet aggregation and antithrombin activity of heparin cofactor in vitro. METHODS: Platelet aggregation assay and chromogenic assay were used in this research. RESULTS: Homocysteine had no effects on platelet aggregation induced by ADP and thrombin or heparin cofactor's antithrombin activity in vitro (P > 0.05). CONCLUSION: There is no correlation between Hcy and the "ADP passway" of platelet aggregation. Thrombosis induced by Hcy does not depend on heparin cofactor's antithrombin activity either.

Adult↗
PubMed1999

[A study on the quality criteria for wushicha capsules].

OBJECTIVE: To build up the quality criteria for Wushicha capsules. METHOD: Rhizoma Atractylodis, Cortex Magnoliae Officinalis, Rhizoma Chuanxiong, Herba Pogostemonis in Wushicha capsules were identified by TLC. The contents of magnolol in the preparation were determined by GC. RESULT: These methods are simple and accurate. CONCLUSION: These methods can be used for the quantitative analysis of Wushicha capsules.

Asteraceae↗
PubMed1999

[Effects of reperfusion on neuronal changes and macrophagic response after transient focal ischemia--reperfusion of brain in rats].

Male SD rats(n = 29) were subjected to 2 hours' middle cerebral artery occlusion and were killed at various times of reperfusion (0.5-48 hours). The histological features of neuronal changes, the macrophagic response and the relationship between them were investigated. The neurons underwent acute changes(shrinking or swollen), and the pyknotic apoptotic neurons were occasionally seen within 3 hours. The number of apoptotic neurons increased with recirculation time. The scattered red neurons and ghost cells were first found at 6 hours of reperfusion. Many apoptotic neurons, apoptotic bodies were detected in the penumbra of ischemic core at 48 hours. Lectin histochemistry with GSI-B4 was performed to observe the macrophage. The results showed the active microglia and macrophage from meninges presented at 12 hours. The macrophages from parenchyma vessels appeared at 24 hours. Reactive microglia and extrinsic macrophage aggregated into the band of macrophage which phagocytized the apoptotic neurons. The results indicate that, firstly, it is important to prevent the neuronal apoptosis and reduce the volume of infarct in stroke patients; secondly, irreversible neuronal lesions simultaneously stimulate microglial activation and the presence of extrinsic macrophages from meninges was earlier than the appearance of the macrophages from parenchyma blood-monocytes. The authors also suggest that the data on the neuronal changes over time after ischemia and reperfusion be useful reference materials for selecting the most timely medication to treat cerebral infarction.

Animals↗
PubMed1999

[An experimental study on transplantation of frozen autogenous tooth-mandible compound].

OBJECTIVE: To study the feasibility of reserving the teeth in the transplantation of frozen autogenous mandible. METHODS: Forty adult Wistar rats were used for these operations of transplantation of frozen autogenous tooth-mandible compound (ATMC) and fresh ATMC. Then the healing process after transplantation of frozen ATMC and fresh ATMC was compared by clinical, radiographical, histological and microangiographical observation. RESULTS: There were no significant differences between the healing process of frozen ATMC and that of fresh ATMC, and the fibres of regenerated periodontal membrane which maintained the dental root in normal position could be imbedded in the newly formed cementum. CONCLUSION: It is feasible to transplant the frozen ATMC for reconstructing the mandible and improving its function in clinic.

Alveolar Process↗
PubMed1999

[Methodological study of measuring flow field uniformity downstream of mitral valve using Doppler echocardiography].

The objective of this study was to establish a quantitative measurement of flow field uniformity of mitral valve in vivo. The flow field uniformity downstream of mitral valve in normal persons and patients with mitral stenosis were studied using advanced color Doppler flow imaging technique. The results showed that a significant difference was found in the quantitative items of flow field uniformity between normal persons and patients with mitral stenosis (P < 0.05). There was significant correlativity between the items of flow field uniformity and other routine items of Doppler echocardiography. The authors conclude that the method is safe, non-invasive, accurate and repeatable, and it can really reflect the flow field uniformity of normal persons and patients with mitral stenosis.

Adult↗
PubMed1999

[Construction of full-length cDNA clone and complete nucleotide sequence of RNA2 of cucumber mosaic virus SD strain].

The full-length cDNA clone of CMV-SD RNA2 was constructed from two cDNA clones, which were obtained by RT-PCR with primers synthesized based on terminal sequences determined using 5' RACE and 3' RACE, respectively; and the complete sequence was determined. It consists of 3048 nt and there are two partial overlapped ORFs. ORF1, which is located in the 5' portion (79-2652 nt), codes for 2a protein of 858aa, and GDD consensus sequence reported to be "polymerase site" was found in deduced 2a protein sequence. ORF2, which is located in the 3' portion (2414-2746 nt), codes for 2b protein of 111aa. The overall nucleotide sequence homologies with RNA2 of CMV Fny and Q strain, which are representatives of subgroups I and II, respectively, are 91.7% and 75.6%; the amino acid sequence homologies are 93.8% and 67.7% for 2a protein, and 83.0% and 51.3% for 2b protein, respectively. These results demonstrated that CMV-SD belongs to subgroup I.

Amino Acid Sequence↗
PubMed1999

[Effects of the centipede aqueous extract on animal gastrointestinal function].

In order to investigate the mechanism of Centipede on the function of animal gastrointestinal digestion, we studied the effects of cetipede aqueous extract on animal digestion, secretion and intestional movement. The results showed that the Centipede extract increased the volume of gastric juice and acid, activity of pepsin, volume of pancreatic juice and protein in rat. The Centipede extract also accelerated the mice intestinal movement. The above results revevaled that Centipede could regulate the function of the gastrointestinal digestion, secretion and movement.

Animals↗
PubMed1999

[Possibility and clinical significance of reservation middle turbinate in endoscopic sinus surgery].

OBJECTIVE: To study the possibility and significance or reservation of the middle turbinate in endoscopic sinus surgery for sinusitis and/or nasal polyps. METHODS: (1) The morphology and mucosal ultrastructure of middle turbinate were observed preoperatively and postoperatively by nasal endoscopy and scanning electron microscopy in 20 cases; (2) The proportions of the septal turbinate formation and the closure of ethmoid sinus cavity were investigated postoperatively by nasal endoscopy in 60 cases with middle turbinate reserved and 60 cases with middle turbinate resected. RESULTS: (1) The morphology and mucosal ultrastructures of reserved middle turbinate were observed to have recovered postoperatively; (2) The proportions of the septal formation and the closure of ethmoid sinus cavity in the groups with middle turbinates reserved and resected were 13.3%, 18.3% and 58.3%, 51.7%, respectively (P < 0.01). CONCLUSION: It is possible that the structure of reserved middle turbinate may return to normal and it is important to save the middle turbinates to improve clinical cure rate.

Adolescent↗
PubMed1999

[Anatomic variations of the ostiomeatal complex and their correlation with chronic sinusitis: CT evaluation].

OBJECTIVE: To explore the relationship between anatomic variations in the ostiomeatal complex (OMC) and chronic sinusitis. METHODS: Coronal plane CT scans of the paranasal sinuses showing the ostiomeatal complex including the middle turbinate, uncinate, ethmoid bulla, agger nasi and Haller cell were analyzed in 297 individuals (594 sides). Statistical analyses were carried out with SPSS 5.0. RESULTS: There were 81.14% of OMC noted to have at least one variation. The anatomic variations included paradoxical curvature of the middle turbinate (13.97%), the pneumatized middle turbinate (34.85%), uncinate hyperplasia(19.36%), deviation of uncinate (45.27%), large ethmoidal bulla (30.30%), large agger nasi (0.70%) and Haller cell (1.00%). The correlation between variations and chronic sinusitis was significant (P < 0.01). CONCLUSION: The occurrence of OMC variation is common. The variations may be one of the causes of chronic sinusitis. Therefore, properly handling the variations was important in the endoscopic sinus surgery.

Adolescent↗