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Biomedical subjects

G Yoshii

Publications and source records attributed to G Yoshii.

31 records · Page 2Linked to original sources
PubMed1976

Effects of irradiation on RNA synthesis primed by calf thymus deoxyribonucleoprotein treated with salt and salt-urea.

1. The effects of ionizing radiation on the activity of calf thymus templates were examined in a Escherichia coli RNA polymerase system. 2. The template activity of native and 2 M NaCl-5M urea-treated deoxyribonucleoproteins was enhanced by relatively low doses of irradiation, while that of 2 M NaCl-treated deoxyribonucleoprotein was not enhanced by irradiation. 3. The template activity of purified DNA was markedly decreased by irradiation, while that of native deoxyribonucleoprotein, 2 M NaCl-treated, and 2 M NaCl-5 M urea-treated ones were slightly decreased at a higher dose range. The doses for 50% inactivation of these templates were 1.3, 210, 140, and approximately 200 krad, respectively.

Animals↗
PubMedDate not supplied

Effect of intensity of 1.2 MHz ultrasound on change in DNA synthesis of irradiated mouse L cells.

Cultured mouse L cells attached to circular plates were exposed to continuous wave 1.2 MHz ultrasound and the amount of 14C-thymidine (TdR) incorporated into cellular DNA was measured. Enhanced 14C-TdR incorporation per cell in attached cells was observed at ultrasonic intensities above 0.5, ISATA (0.8, ISPTA) W/cm2, although the total amount of incorporated 14C-TdR per sample decreased with increasing exposure energy at intensities above 1.5 (2.8) W/cm2. The presence of cavitation was evidenced by three kinds of physicochemical dosimeters; the liberation of iodine from KI-starch solution, a decrease in DNA molecular weight and sucrose hydrolysis. Collapse cavitation was detected at intensities above 1.5 (2.8) W/cm2 and stable cavitation was observed at 0.5 (0.8) W/cm2. These results suggest that the change in DNA synthesis of irradiated mouse L cells takes place independently of collapse cavitation, and may be due to stable cavitation.

Animals↗