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Biomedical subjects

G Winter

Publications and source records attributed to G Winter.

At least 199 records · Page 11Linked to original sources
PubMed1981

[The electrophoretic mobility test in cancer diagnosis at the 1st Women's Clinic of Vienna University].

The electrophoretic mobility test (EMT), modified only slightly, was applied to a group of 25 patients suffering from gynecological cancer as well as to a control group of the same number. On an average, cancer patients demonstrated an inhibition of 5.5%, control patients an inhibition of 2.3%. These mean values near the discrimination border are caused in 14 false (positive or negative) results and in further 8 results valued ambiguous at inhibition quotes between 3 and 5%. Neither methodological nor casuistical analyses yielded clear conclusions about the reasons of recurrent test failures. Therefore, the manner of measurement becomes more interesting for us as more as this method does not include objectivity and precise documentation of the physical proceedings in a sufficient range. At least, we refer to an alternative method of measurement equalizing these problems by photographical documentation.

Adolescent↗
PubMed1980

Cloning of influenza cDNA ino M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein.

A strategy has been developed for sequencing the single-stranded RNA genes of influenza virus. Restriction fragments derived from double-stranded cDNA copies of total influenza RNA were cloned into the bacteriophage M13mp2 and sequenced by the dideoxy technique. Sequences were extended and overlapped by using the virion RNA as template and priming with small restriction fragments. In the course of this strategy, the nucleotide sequence of segment 7 (1027 nucleotides) was completed and provides the primary structure of the matrix protein (27, 861 daltons). In addition, there is a second long reading frame which partly overlaps the reading frame of the matrix protein.

Base Sequence↗
PubMed1978

[Diagnosis and treatment of trauma to blood vessels around the knee joint (author's transl)].

Eighteen arterial reconstructions after trauma in the region of the knee joint were performed between 1970 and 1976. In seven cases there had been fracture of the distal femur, in five of the head of the tibia, in three dislocation of the knee joint or severe contusion of the popliteal artery. In 12 cases referral had been eight hours or later after injury. The concomitant vessel injury was not recognised in six. Vascular reconstruction by direct suture with a venous patch was performed in eight, interposition of a venous autograft in five, and saphenous vein bypass in four. Results depended upon length of ischaemia time and the level of the vascular injuries. Complete restitution to normal was achieved in only two cases, while in the remaining 12 there were defects of motor and sensory function as seen in the post-ischaemia syndrome. In four cases above-knee amputation was necessary despite successful reconstruction of the artery because there was irreversible ischaemic damage with tissue necrosis. To improve these results careful examination of blood vessels and, in questionable cases, angiography is recommended for every trauma victim.

Amputation, Surgical↗
PubMed1978

3'End labelling of RNA with 32P suitable for rapid gel sequencing.

A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with aniline, leaving the RNA terminally labelled with 3' 32P-phosphate. When applied to a model compound, tRNAPhe from E. coli, over 28 residues could be read from the 3' end.

Adenosine Triphosphate↗
PubMed1978

[Gallstone composition and dissolution by chenodesoxycholic acid (author's transl)].

208 gallstones were collected in sequence and subjected to chemical analysis. Only 10.6% of these stones turned out to be purely composed by cholesterol and only these can be considered to be suited for therapy with chenodesoxycholic acid (CDC). Gallstones composed exclusively by cholesterol cannot be identified in vivo; therapy with CDS has to be followed closely by multiple X-ray and laboratory examinations; in addition there are many relapses after therapy with CDC. For these reasons we still tend to prefer surgery as the therapy of choice in gallstone disease.

Chenodeoxycholic Acid↗
PubMed1978

[Intraoperative bacterial contamination and posttraumatic wound infection (author's transl)].

In a consecutive series of 150 fractures treated surgically 16 wound infections were observed. Evaluating the intraoperative wound swabs, type and localisation of the fractures, duration of operation and tourniquet time it was tried retrospectively to find a relationship to these posttraumatic wound infections. The highest rate of infection was found in fractures of the tibia. Open fractures had a higher risk than closed ones. Furthermore, a prolonged duration of operation was related to a rising risk of infection. The intraoperative wound swabs themselves did not allow any conclusion concerning the risk of postoperative infection. Postoperative wound infections appeared nearly as frequent after negative as after positive intraoperative swab. Only the species of bacterias found intraoperatively allowed a conclusion concerning subsequent wound infection so far that after an intraoperative finding of gram-negative bacterias and - surprisingly - apathogenic sporogenic bacterias postoperative wound infections were observed more frequently than after finding other bacterias. In summary, however, the routine intraoperative wound swab does not seem to be suitable as a sreening-test for early recognation of impending postoperative wound infections.

Bacterial Infections↗
PubMed1975

[Duplication of the external and common iliac veins with a venous trunk anterior to the artery (author's transl)].

Short duplications of the stem forming an island where the artery crosses it have been described as isolated variants of the v. iliaca communis. V. iliaca externa and interna opening into the vena cava separately and a femoral vein opening into the ipselateral internal iliac vein are isolated observations of irregular junctions. An observation of duplication of the v. iliaca externa and to some extent also of the v. iliaca communis with concomitant duplication of the v. femoralis is reported, one of the two iliac venous trunks taking a course ventral to the external and common iliac arteries. These numerical abnormalities in combination with the irregular position of the v. iliaca externa and partly also of the common iliac vein are observed here for the first time.

Abnormalities, Multiple↗
PubMedDate not supplied

Engineering of tyrosyl tRNA synthetase.

The gene encoding the enzyme tyrosyl tRNA synthetase from Bacillus stearothermophilus has been systematically altered using synthetic oligonucleotides as mutagens. The construction of mutations has been facilitated by using strains of bacteria defective in mismatch repair and also by utilising a genetic marker in the M13 strain (such as an amber mutation, or an EcoK or EcoB site) which allows selection for the progeny of M13 replication derived from the minus (mutagenized) strand. Several mutations have been constructed in the ATP binding site to elucidate the roles of individual residues in catalysis and substrate binding and it has even been possible to construct mutants which have improved affinity for ATP. Mutations in various surface lysine and arginine residues have allowed us to identify potential contacts with the tRNA, and indicate that a cluster of basic residues close to the C-terminus of the enzyme probably makes important interactions with the tRNA.

Amino Acyl-tRNA Synthetases↗