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Biomedical subjects

G Wilcox

Publications and source records attributed to G Wilcox.

87 records · Page 5Linked to original sources
PubMed1974

Direction of transcription of the regulatory gene araC in Escherichia coli B-r.

The protein product of the regulatory gene araC can be synthesized in a cell-free, protein-synthesizing system programmed with a lambdaparaC(+)B DNA template. Hybrid, renatured phage DNA molecules prepared with DNA from phages lambdaparaC(+)B and lambdaparaC3B (araC3 is a nonsense mutation) were used to program the cell-free synthesis of the araC protein. The findings observed lead to the conclusion that the codogenic strand of the araC gene is on the light strand of the phage DNA. The araB gene is on the heavy strand, as determined by DNA.RNA hybridization. Thus, with regard to the standard E. coli map, araC is transcribed in a clockwise direction, whereas transcription of the araBAD operon has a counterclockwise orientation. The technique described should allow one to determine the direction of transcription of any gene that can be incorporated into the genome of a specialized transducing phage.

Arabinose↗
PubMed1971

Purification of the araC protein.

The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.

Aniline Compounds↗
PubMed1971

Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r.

An increase in the rate of synthesis of ara-specific messenger ribonucleic acid as measured by deoxyribonucleic acid-ribonucleic acid hybridization has been detected in the induced wild-type (ara(+)) strain of Escherichia coli B/r as compared with the uninduced control, thus providing evidence that regulation of the positively controlled l-arabinose operon is at the level of transcription.

Arabinose↗
PubMedDate not supplied

The use of decision support systems in health facility allocation problems.

Employing Kitchener's Freeport Hospital as a case study, the author introduces decision support systems as possible aids for allocating public health care facilities. The study found that the Dynamic Interactive Network Analysis System (DINAS) was useful in determining Kitchener as the most suitable location for Freeport, based upon a comparison of need throughout Ontario.

Catchment Area, Health↗
PubMedDate not supplied

Male models.

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Administrative Personnel↗