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Biomedical subjects

G Weryha

Publications and source records attributed to G Weryha.

16 recordsLinked to original sources
PubMed1992

[Contribution of intra-operative measurement of intact parathormone in surgery for primary hyperparathyroidism].

Using a sensitive two-site immunoradiometric assay which detects intact parathormone (iPTH), we studied the decrease in peripheric and jugular plasmatic iPTH during surgical removal of abnormal parathyroid (s). In the next future, results of intact parathormone (iPTH) assay will be given in 45 minutes. In a prospective study of 33 patients operated on for hyperparathyroidism or for cold thyroid nodule, the serum levels of intact PTH was measured intraoperatively in peripheric and in jugular blood. The preoperative mean serum iPTH concentration was 119.23 +/- 172.48 pg/ml and fell to 34.5 +/- 32.21 pg/ml after surgery in 14 cases of primary hyperparathyroidism (p < 0.001). Thirteen out of 14 patients had serum iPTH values less than 65 pg/ml within 15 minutes after parathyroidectomy. The preoperative mean serum iPTH concentration in the 5 secondary hyperparathyroidism was 781.2 +/- 403.19 pg/ml. This value fell to 124 +/- 66.91 pg/ml after parathyroidectomy (p < 0.04). No significant decrease was observed in the mean serum concentration of the 14 patients operated on for cold thyroid nodule. Patients suffering from single parathyroid adenoma presented a significant gradient in jugular plasmatic PTH concentration between the adenoma side and the contralateral one. This gradient decreased during effective parathyroid adenomectomy (309.7 +/- 313.3 pg/ml to 3.7 +/- 35.1 pg/ml). Intraoperative serum iPTH concentration will provide a valuable tool to appreciate the effectiveness of surgical removal of parathyroid glands and to detect the location of parathyroid adenoma when the surgical research is negative.

Adult↗
PubMed1991

Autosomal dominant arthropathy in a French family.

We studied a French family in which 18 adult members had destructive arthropathy and enthesopathic changes. In 16 patients, the diagnosis was verified radiologically, and in the 2 other patients, the clinical history was consistent with familial arthropathy. The disease predominantly affects the wrists, fingers, shoulders, and peripheral entheses, and its onset occurs after age 18. Genetic transmission is autosomal dominant, with 100% penetrance, and is not related to the HLA system. The clinical and radiologic features are strikingly similar in all patients in successive generations and different branches of the genealogic tree, and this suggests monogenic transmission.

Age Factors↗
PubMed1991

Dynamic changes in soluble interleukin-2 receptor levels during treatment of Graves' disease. Correlation with disease activity.

The activation of T lymphocyte is accompanied by the release of soluble interleukin-2 receptors (sIL-2R) which can be assessed in biological fluids. A prospective study of the dynamic changes in sIL-2R levels was performed in the serum of 10 patients undergoing a medical treatment for Graves' disease. All patients received carbimazole during the study and, when necessary, L-thyroxine to compensate hypothyroidism. sIL-2R levels were measured before (M0) and after the 1st (M1), 3rd (M3) and 6th month (M6) of treatment. The levels of sIL-2R were high at M0 and M1, and decreased significantly between M1 and M3 (p = 0.03). At M0, the levels of sIL-2R were highly correlated with triiodothyronine (T3) levels (p = 0.0003), early [131I] uptake (p = 0.007) and, to a lesser degree, with anti-thyrotropin receptor antibody levels (p = 0.02). At M6, no correlation was found anymore. We conclude that sIL-2R levels are increased in patients with untreated Graves' disease. They are highly correlated with the markers of Graves' disease activity and decrease during medical treatment.

Adult↗
PubMed1991

[Bone densitometry: technical principles and practical value].

X-ray dual photon absorptiometry has replaced isotopic mono and dual photon absorptiometry in the assessment of metabolic bone diseases. This method is a valuable tool for epidemiologic studies. Individual examinations give poor in vivo accuracy and precision. Further technical improvements are needed to give bone densitometry a predictive value for osteoporotic risk.

Absorptiometry, Photon↗
PubMed1990

[Bone complications of fluorotherapy: influence of androgen deficiency].

The use of sodium fluoride for treatment of osteoporosis has led to the arousal of particular complications: osteomalacia and cortical bone fractures which are enhanced by preexisting bone diseases. We report the case of a 55 years old female suffering from autoimmune Addison disease since 10 years and menopaused since 7 years. After an 18 month treatment by sodium fluoride for osteoporosis she presented radiographic, scannographic and scintigraphic evidences of unexpected stress fractures of several ribs, of the sacral part of the left sacro-iliac joint, of the symphyseal joint. The sodium fluoride treatment associated to the lack of adrenal androgens are responsible for the onset of pelvic stress fractures which usually occur in elderly subjects.

Androgens↗
PubMed1990

Scanning electron microscopy (SEM) of the human thyroid gland: a technical improvement for follicular cell observation.

SEM is a valuable tool for the examination of hollow organ free surfaces. It has been used in a few studies of human thyroid. The cell surface observation is usually limited by the presence of colloid in the follicular lumen. A technique of direct removal of the colloid has been previously proposed. It gives good morphological results. But this method needs preliminary fixation and critical point drying of thyroid samples. Antigenic properties of tissue blocks are then unsuitable for further staining. We describe a fast and easy technique for colloid removal by washing thyroid samples in a saline solution. Colloid is correctly removed from the tissue block surface prior to fixation. Apical cells are left undamaged. The exposure of thyroid blocks to ultrasound did not improve colloid removal. A post-fixative chemical drying in hexamethyldisilazane shortens processing time. We have studied the efficiency of this technique on 24 human thyroid samples obtained by surgery (normal tissue, Grave's disease, heteromultinodular goiter, toxic solitary adenoma). All specimens were observed by SEM and transmission electron microscopy in order to assess the good preservation of cell surface details. This easy technique could be used as a routine process.

Adenoma↗
PubMed1989

Stress and auto-immune endocrine diseases.

Auto-immunity may occur in all endocrine tissues, with a particular prevalence in thyroid and pancreatic islets. The most demonstrative clinical expressions of this auto-immunity are Graves' disease and insulin-dependent diabetes. In the former, extensive data are available upon the immunological disorders seen in peripheral blood as well as in the thyroid itself. The predisposal profile of such diseases is quite well delineated (genetic markers in HLA system, organ-specific T suppressive deficiency). On the other hand, the mechanisms implicated in the onset of auto-immunity are still questioned, the classical viral infection being scarcely demonstrated. Clinical observation registers frequent stressful life events just before the onset of these diseases, but there are few convincing data in the literature. The recent findings on the close relations between the immune system and the central nervous system lead to conceive an actual psychoneuro-endocrine-immune axis. This axis might explain the eventual priming effect of stress in patients predisposed for an organ-specific auto-immunity.

Autoimmune Diseases↗