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Biomedical subjects

G Wei

Publications and source records attributed to G Wei.

At least 73 records · Page 4Linked to original sources
PubMed1998

[The protective effects of nerve growth factor on peripheral nerve injury].

The protective effects of nerve growth factor (NGF) on peripheral nerve injury and sensory neuron growth were studied in three models: peripheral sympathetic nerve injury induced by intraperitoneal injection of 6-hydrodopamine (6-OHDA), mechanically injured ulnar nerve and cultured dorsal root ganglion (DRG) of newborn rats in vitro. The results indicate that NGF dose-dependently increased norepinephrine content in mouse submandibular gland and cell numbers of C7 (seventh cervical vertebra), T1 (first thoracic vertebra) dorsal root ganglion in rabbits, and also dramatically promoted the growth of neuronal projections in cultured DRG. These results suggest that NGF has significant neuroprotective action in peripheral nerve injury and a strong neuronutrition on sensory neuron.

Animals↗
PubMed1998

[Math-model of efficient cultivation of Angelica sinensis].

Using two-time general rotation-constitution design, the math-mdele has been founded about Angelica sinensis prodution efficiency and quantity of nitrogen, phosphorus and potassium to use by doing experiments in many different spots and production demonstration for testing and verifying. Accordin to the modle, computer is used to simulate the better, through which people can get fresh Chinese angelica over 500 Kg per mu and also net income over 500 yuan per mu. It shows that this is the best combination. 95% density riliability for this plan is 6860-7600 plants, pure nitrogen 18.75-22.2 Kg, P2O5 9.1-11.1 Kg and K2O 4.46-5.37 Kg One mu. Proportion for nitrogen, phosphorus, potassium is 1:0.49:0.24.

Angelica↗
PubMed1997

Hepatitis C virus (HCV)-induced IgG-IgM rheumatoid factor (RF) complex may be the main causal factor for cold-dependent activation of complement in patients with rheumatic disease.

A low serum complement level is commonly found in patients with rheumatic diseases. We evaluated 170 patients with such diseases to determine their serum levels of CH50, C3 and C4 protein. Persistent hypocomplementaemia was found in 19 of those patients, particularly in those with systemic lupus erythematosus (SLE). Cold-dependent activation of complement (CDAC) was demonstrated in nine of the 19 (47.4%), and six of the nine patients demonstrated infection with HCV (66.7%). The nine patients that exhibited CDAC had nearly normal haemolytic complement activity when the sera were separated either at 37 degrees C or in EDTA-treated plasma. Conversely, it markedly decreased, even to the point of being immeasurable, when the sera were separated at 4-21 degrees C. No significant deficiency in C3 and C4 protein levels was found in these patients. Clinical parameters other than levels of anti-HCV antibody, transaminase, and RF were not influenced by CDAC. In an attempt to isolate the causal factor for CDAC, we isolated IgG fractions from the CDAC patients by using a protein G column, in which case precipitates were collected from the eluates. The precipitates were mixed with normal serum and incubated at 4-21 degrees C for 18 h. A decrease in the level of CH50 in normal serum was observed, which predominated (P < 0.001) when precipitates from HCV-infected patients were used. This indicated CDAC was possibly interrelated to the precipitates of such patients. This precipitate was proved to contain IgM besides IgG. It is therefore possible that an HCV-related IgG complex or an IgG-IgM RF complex may be formed at low temperature and be involved in activating the complement system in vitro.

Adult↗
PubMed1997

Effect of tetrandrine on proto-oncogene c-fos expression in rat cerebrum.

AIM: To detect the effect of tetrandrine (Tet) on c-fos gene expression in cerebrum induced by lindane, a neurotoxicant which activates Ca2+ channels. METHODS: Northern and dot blotting, dual wavelength thin layer chromatography scanner, were used in this study. RESULTS: Lindane 30 mg.kg-1 given by intragastric gavage (i.g.) increased the expression of c-fos gene to 146 mm2 in rat cerebrum 1 h after treatment. Tet 1, 2, and 4 mg.kg-1 given by i.g. 30 min prior to lindane reduced c-fos gene expression in a concentration-dependent manner. Expressed genes reached only 86, 40, and 39 mm2, respectively. CONCLUSION: Tet inhibited c-fos gene expression in rat cerebrum induced by Ca2+ agonist-lindane.

Alkaloids↗
PubMed1997

[Ultrastructure of adherence of Streptococcus mutans MT8148 and its glucosyltransferase deficient mutants].

The ultrastructure of adherence of Streptococcus mutans MT8148 and its glucosyltransferase deficient mutants were compared by Scanning Electronic Microscope. Four patterns of adherence of tested strains in the presence of sucrose were observed. These results suggest that the cell-associated GTase is responsible for firm aggregation of cells. However, cell free GTase is also important in aggregation between cells.

Bacterial Adhesion↗
PubMed1997

[Effects of sodium doecyl sulfate on the artificial dental plaque in chemostat].

Sodium doecyl sulfate (SDS) is widely used as a detergent in dentifrices. It has been shown to interfere with the protein adsorption to hydroxyapatite (HA), and inhibit acquired pellicle formation. The aim of the present study was to examine the effects of SDS on the artificial dental plaque in chemostat. The amount of the 3H-labelled bacteria adhered on the enamel fragment surface was determined with scintillometer. The artificial dental plaque was observed under the scanning electron microscope. The results showed that enamel fragments treated with SDS adsorbed less bacteria than untreated ones, and had no plague formed. It suggested that SDS can inhibit the bacterial adherence on enamel surface and thus reduce dental plaque formation.

Actinomyces viscosus↗
PubMed1997

Expression of GCF protein is dependent on the cell cycle.

GCF is reported to be a transcriptional regulator that binds to specific GC-rich sequences in the epidermal growth factor (EGF) receptor gene promotor and to represses its transcription. In this study, to examine the expression of GCF in cell cycle, we have developed antibodies against GCF protein. The anti-GCF antibody recognized a major band with a molecular weight of approximately 100 kda present in human Hela cell line by immunoprecipitation followed by Western blotting. Although GCF proteins were detected throughout the cell cycle, it reached the highest level in G1 phase, and fell down to the lowest level during G2/M phase. Thus, the expression of GCF is regulated in a cell cycle-dependent manner, suggesting a potential role of GCF in the control of cell growth.

Binding Sites↗
PubMed1997

Re-examination of 5' end of cDNA of transcription factor GCF.

N-terminal region of transcriptional factor GCF was re-examined as the published amino acid sequence is extremely basic. 5'Rapid Amplification of cDNA End (5' RACE) analysis was performed and the result revealed that sequence of the first 308 bases of published GCF cDNA does not conform to the one obtained by 5' RACE. Sequence of genomic GCF DNA is consistent with the new cDNA sequence but not with the published one. Results of ordinal RT-PCR analyses are consistent with that of 5' RACE.

Base Sequence↗
PubMed1997

[Impedance of cerebrovascular system simulated by a hemodynamic model of brain circulation].

A hemodynamics model of cerebral Willis circulation is proposed. The model consists of the compensation of blood flow as well as the compliance, resistance and inductance of arteries. The impedances of carotid and vertebral arteries are obtained by solving the govern equations. The theoretical impedances correspond well with the experimental results. The simulating results demonstrate that the alteration of the arterial dynamic parameters influences not only the ipsilateral impedance but also the contralateral arterial system. These conclusions suggest that the carotid or vertebral impedance is not suitable for describing the behaviour of its own arterial system, because the impedance is also influenced by the other arterial system and blood pressure.

Animals↗
PubMed1996

Percutaneous absorption of ketoprofen from different anatomical sites in man.

PURPOSE: The purpose of this study was to investigate the percutaneous absorption of ketoprofen applied topically to different anatomical sites on the body. METHODS: The study design was a randomized, four-way crossover in 24 healthy male subjects. One gram of ketoprofen 3% gel (30 mg dose) was applied every six hours for 25 doses over a 100 cm2 of the back, arm, and knee. A 0.5 ml of ketoprofen solution (60 mg/ml) was applied to the back as a reference treatment. Plasma and urine samples were obtained for the assay of racemic ketoprofen and ketoprofen enantiomers (S and R), respectively. RESULTS: The relative bioavailabilities of ketoprofen gel were 0.90 +/- 0.50, 1.08 +/- 0.63, and 0.74 +/- 0.38 when applied to the back, arm, and knee, respectively. The plasma ketoprofen C(max) for gel applied to the back and arm are similar (p > 0.05) but C(max) was lower when applied to the knee (p < 0.05). The time to C(max) ranged from 2.7 to 4.0 hours and was similar for gel treatments on the back and arm, but no longer for the knee treatment. The fraction of dose excreted in urine as total S and R enantiomers ranged from 5.41 to 9.10%. CONCLUSIONS: The percutaneous absorption of ketoprofen was similar when applied to either the back or arm but was lower when applied to the knee.

Administration, Cutaneous↗