Increased UDP kinase activity in rat and human hepatomas.
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Biomedical subjects
Publications and source records attributed to G Weber.
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The authors collected data from the literature and from experts on the subject of vaccination of patients with dermatologic disorders. They assembled these data into categories of specific skin conditions and vaccinations. These data may be used in deciding whether or not to vaccinate, and at which stage of the dermatologic condition.
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The behavior of glutamine-phosphoribosylpyrophosphate amidotransferase (amidophosphoribosyltransferase, EC 2.4.2.14) was determined in normal, differentiating, and regenerating liver and in a spectrum of hepatomas of widely different growth rates. The liver and tumor enzymes were measured in 100,000 x g supernatants prepared from 20% tissue homogenates containing 0.25 M sucrose and 1 mM MgC12. Kinetic studies were carried out on the amidotransferase in the curde supernatant from liver and rapidly growing hepatoma 3924A so that under optimum standard assay conditions only the enzyme amount would be the limiting factor. The kinetic results showed that certain properties of the amidotransferase from liver and hepatoma were similar. The liver and hepatoma enzyme exhibited apparent Km's for: glutamine, 1.7 and 2.3 mM; MgC12, 0.7 and 1.1 mM, and phosphoribosylpyrophosphate. S0.5 for 0.9 and 0.4 mM, respectively...
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