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Biomedical subjects

G Wauters

Publications and source records attributed to G Wauters.

At least 55 records · Page 3Linked to original sources
PubMed1994

Short-comings of irgasan ticarcillin chlorate broth for the enrichment of Yersinia enterocolitica biotype 2, serotype 9 from meat.

Recovery of Yersinia enterocolitica serotype 0:9 from artificially contaminated minced pork with the enrichment medium irgasan ticarcillin chlorate broth (ITC) was poor. This was due to the lower growth rate of this serotype in comparison with serotype 0:3. Tests on the behaviour of serotype 0:3 and 0:9 towards some selective agents in ITC broth showed that the minimal inhibitory concentration of chlorate was much lower for serotype 0:9 than for serotype 0:3. Omitting chlorate in the ITC enrichment stimulated the growth of pure cultures. Reduction of MgCl2 and malachite green to 80% of the original concentration further increased the growth, so that after 48 h of incubation similar counts (10(7)-10(8) cfu/ml) as for serotype 0:3 were obtained.

Animals↗
PubMed1994

An outbreak of Ochrobactrum anthropi bacteraemia in five organ transplant patients.

Nosocomial bacteraemia caused by Ochrobactrum anthropi occurred over a 1-month period in five organ transplant recipients, four of whom were in the same renal and pancreatic transplant unit. Bacteraemia occurred with cyclosporin A, azathioprine and steroids, and with a rabbit anti-thymocyte globulin (RATG) during the induction phase. RATG appeared to be the only common factor among the five cases. Over the period described, 71.4% of all patients receiving RATG developed O. anthropi bacteraemia. Three patients presented with fever and chills during or shortly after RATG infusion. Analysis of residues of the infusion, and the used vials of RATG, showed the presence of O. anthropi in concentrations of between 20 and 1000 cfu ml-1 in 5.3% of samples. Unused vials were found to be heavily contaminated with either O. anthropi or Microbacterium spp. in 23.5% of samples. All positive vials were of one particular lot number suggesting a malfunction in the manufacturing process. Many parenteral drugs such as the RATG used here do not contain preservatives and, although aseptically prepared, will not withstand thermal sterilization. Bacterial contamination of these small volume medications is not always easily detectable by conventional methods. This outbreak highlights the need for accurate quality control testing to detect small inocula that may occur during or after the manufacturing process.

Adult↗
PubMed1994

Emergence of high-level resistance to glycopeptides in Enterococcus gallinarum and Enterococcus casseliflavus.

Enterococcus gallinarum BM4231 and Enterococcus casseliflavus BM4232, isolated from the feces of a patient under oral therapy with vancomycin, were resistant to high levels of vancomycin (MICs of > 256 micrograms/ml) and teicoplanin (MICs of 128 and 64 micrograms/ml, respectively). This phenotype is new for these bacterial species that are naturally resistant to low levels of vancomycin and appears to be due to in vivo acquisition of plasmid pIP218 carrying the vanA gene cluster.

Anti-Bacterial Agents↗
PubMed1993

[Parasitological evaluation of the stool].

The parasitologic investigation performed without clinical orientation in a routine laboratory allowed to identify the most common parasites, often cysts of protozoa, using a minimal technique. A more accurate investigation (e.g.: concentration, extraction and flotation) was hampered by a too large number of systematic specimens. The use of an immersion objective improves the detection and identification of cysts of protozoa. There is still some controversy on the virulence of small amoebae, flagellates, Blastocysts and Entamoeba coli, the infection being often massive. AIDS should predispose to new parasitic diseases such as cryptosporidiosis and strongyloidosis. A careful examination of stools for parasites has become mandatory in view of the wide geographical origin of patients with a parasitic disease.

Animals↗
PubMed1993

Identification of vancomycin-resistant lactic bacteria isolated from humans.

By using cell morphology, arginine dihydrolase, and gas production in de Man, Sharp, Rogosa broth, 122 isolates of vancomycin-resistant lactic bacteria from humans were assigned to five profiles, allowing us to distinguish Pediococcus, homofermentative and heterofermentative Lactobacillus, and Leuconostoc species. The absence of L-(+)-lactic acid, as detected spectrophotometrically, was confirmatory for Leuconostoc species. API 50 CHL panels were useful for the identification of Lactobacillus species.

Drug Resistance, Microbial↗
PubMed1993

Severe human infections caused by Vibrio metschnikovii.

Vibrio metschnikovii is largely distributed in the aquatic environment; human infections are rarely observed. A fatal case of septicemia in a patient with liver cirrhosis, renal insufficiency, and diabetes is described. A second case in a 82-year-old woman with septicemia, respiratory problems, and infected leg lesions is reported; she was successfully treated.

Aged↗
PubMed1992

Three cases of opportunistic infection caused by propionic acid producing Corynebacterium minutissimum.

Propionic acid producing strains of Corynebacterium minutissimum were isolated from three patients with opportunistic infections. One neutropenic patient was undergoing chemotherapy for prolymphocytic leukemia; the other two patients were undergoing hemodialysis and peritoneal dialysis respectively. An unusual feature of these three strains was their resistance to several antibiotics, which is seldom seen in diphtheroids other than Corynebacterium jeikeium and CDC group D2.

Aged↗
PubMed1992

Isolation of Arcanobacterium haemolyticum from patients with pharyngitis in Belgium.

During a 40 months period, 49 Arcanobacterium haemolyticum strains were isolated from 12,000 throat swabs of patients with pharyngitis originating from 5 belgian provinces. All strains were recovered on sheep blood Columbia agar plates, supplemented by nalidixic acid and colistin. In two thirds of the cases, pharyngitis was associated with a rash. Half of the cases occurred in the age group between 10 and 20 years and isolates were twice more frequent in females than in males. Streptococcus pyogenes was isolated 23.2 times more frequently than Arcanobacterium haemolyticum.

Adolescent↗
PubMed1992

[Solitary splenic metastasis of endometrial adenocarcinoma. A case report and review of the literature].

The authors report one case of solitary metastatic endometrial carcinoma of the spleen. Carcinomatous metastatic involvement of the spleen usually indicates widespread malignant disease. Solitary metastatic lesions in this organ are exceedingly rare and the literature reveals up today only three cases having a endometrial origin. The authors take this opportunity to make a review of the literature.

Adenocarcinoma↗
PubMed1991

Causative role of Yersinia and other enteric pathogens in the appendicular syndrome.

In 2,861 consecutive patients undergoing appendicectomy for clinically suspected appendicitis an enteric pathogen was isolated from the appendix in almost 7% using an optimal combination of culture media. The pathogenic Yersinia enterocolitica serotypes 03 and 09 predominated (3.6%), followed by Campylobacter and nontyphoid Salmonella. The same pathogen was isolated from the stool in 72.5% of patients with a culture-positive appendix and in 84.1% of those positive for a pathogenic Yersinia. Conversely, no pathogenic Yersinia were isolated in 326 gynaecologic control patients, in whom a normal appendix was removed. No frank appendicitis but mesenteric adenitis and/or terminal ileitis were found in 62.3% of 138 patients with a culture positive appendix, and in 74.6% of those positive for a pathogenic Yersinia. Histologic findings available in 135 patients showed acute suppurative appendicitis in only six (4.5%) patients, and in only one of 73 (1.4%) positive for a pathogenic Yersinia. In contrast, 46.8% of a group of 345 culture-negative appendices showed acute inflammation. A positive stool culture in a patient with suspected appendicitis, if consistent with sonographic and clinical findings, should be taken as strong evidence against the presence of true appendicitis.

Adolescent↗
PubMed1991

Fatal Capnocytophaga canimorsus septicemia in a previously healthy woman.

A previously healthy 47-year-old woman presented to the emergency department with septic shock five days after a small dog bite on the dorsum of her hand. Capnocytophaga canimorsus was isolated from blood cultures. Despite intensive therapy, multiple organ failure developed, and the patient died 27 days after admission. Characteristics of Capnocytophaga (formerly CDC group Dysgonic Fermenter-2) infection are briefly discussed. This unusual outcome in a previously healthy patient and the need for careful management of dog bite wounds, even if initially very small, is emphasized.

Animals↗
PubMed1991

Fatal septicemia with Capnocytophaga canimorsus in a compromised host. A case report with review of the literature.

Capnocytophaga canimorsus (DF-2) is a newly described gram-negative bacterium that can produce serious infections following dog bites in the immunocompromised host. We are reporting a case of C. canimorsus infection in an elderly patient with a history of chronic respiratory disease and alcohol abuse. We also give a review of the clinical spectrum of the infection, discuss the difficulty in establishing the diagnosis and the preferential treatment.

Acute Kidney Injury↗
PubMed1991

Bone marrow scintigraphy in prostatic carcinomas.

Bone marrow scintigrams (MS) were performed on 73 men with histologically proven prostatic carcinomas. Follow-up data were available in 24 cases. Thirty-six patients had skeletal metastases at the time of the first MS investigation. They were compared with conventional bone scintigrams (BS) as well as clinical, biological, radiological and other follow-up data obtained for the same patients. At the present stage of revision and of follow-up of our patients, MS appeared less sensitive than BS in diagnosing skeletal metastases (94% as against 100% if all abnormal MS and BS presentations are considered as diagnostic or 83% as against 86% if more restrictive analytic criteria were applied). On the other hand, MS showed slightly higher specificity (81% for MS as against 73% for BS) when all abnormal MS and BS presentations are considered as diagnostic. With more restrictive diagnostic criteria, BS remains more specific (100%) than MS (87%). Nevertheless, the likelihood of bone metastasis is low when a normal MS is found in case of dubious BS situations, but high when the MS is pathological. In 11 patients with skeletal metastasis for whom follow-up investigations were available, MS appeared better than (4 cases) or equivalent to (6 cases) BS for evaluating the responses and the ultimate evolution of the disease. Marrow scintigrams are thus a useful tool in cases of dubious BS and for evaluating the response of skeletal metastasis to treatment.

Aged↗
PubMed1991

Pathogenicity of Yersinia kristensenii for mice.

Forty-seven strains of Yersinia kristensenii from widely differing sources, representing all known O serogroups of this species, were investigated for virulence with a variety of animal and in vitro assays. Twenty-four (51%) of the isolates were lethal for mice pretreated with iron dextran. Mouse-lethal strains occurred predominantly within O serogroups O:11, O:12,25, and O:16. Virulent Y. kristensenii strains generally did not express the virulence-associated phenotype (Ca2+ dependence and binding of Congo red and crystal violet) which characterizes virulent strains of Y. enterocolitica, nor did they carry the Yersinia virulence plasmid. Although all strains hybridized with a DNA probe derived from the inv (invasin) gene of Y. enterocolitica, none was able to invade HEp-2 epithelial cell culture. Y. kristensenii strains were virulent only when inoculated parenterally into iron-loaded mice. Animals infected in this way succumbed rapidly to infection, generally within 24 h. This finding suggested that the pathogenicity of these bacteria may be attributable to a secreted toxin, but a search for such a substance and for other in vitro correlates of pathogenicity was unsuccessful. These observations indicate that some strains of Y. kristensenii kill mice by a mechanism not previously recognized in yersiniae.

Animals↗
PubMed1991

Fatal Streptococcus suis meningitis in man.

A case of Streptococcus suis meningitis is observed in a 39-year-old and previously healthy meat factory worker. Neurological recovery was incomplete despite adequate and sustained antimicrobial therapy. Early deafness was demonstrated by brainstem auditory evoked potentials. Death due to aspiration pneumonia, cardiac arrest and subsequent cerebral anoxia occurred late in the course of the illness.

Adult↗