Search PubMed⌕ Search

Biomedical subjects

G Warren

Publications and source records attributed to G Warren.

180 records · Page 10Linked to original sources
PubMed1977

Complementation of transfer deficient ColE1 mutants.

The transfer defect of some ColE1 mutants is complemented by ColE1 or ColK, but not by ColE2. This implies that at least one ColE1-specified protein or RNA is normally needed for ColE1 conjugal transfer. The gene(s) postulated for this function lies within a region whose length is at most 50% of the genome.

Ampicillin↗
PubMed1971

Plaster casts.

Explore the source record for details and available documents.

Calcium Sulfate↗
PubMedDate not supplied

Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus.

In the eukaryotic cell, both secreted and plasma membrane proteins are synthesized at the endoplasmic reticulum, then transported, via the Golgi complex, to the cell surface. Each of the compartments of this transport pathway carries out particular metabolic functions, and therefore presumably contains a distinct complement of membrane proteins. Thus, mechanisms must exist for localizing such proteins to their respective destinations. However, a major obstacle to the study of such mechanisms is that the isolation and detailed analysis of such internal membrane proteins pose formidable technical problems. We have therefore used the E1 glycoprotein from coronavirus MHV-A59 as a viral model for this class of protein. Here we present the primary structure of the protein, determined by analysis of cDNA clones prepared from viral mRNA. In combination with a previous study of its assembly into the endoplasmic reticulum membrane, the sequence reveals several unusual features of the protein which may be related to its intracellular localization.

Amino Acid Sequence↗