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Biomedical subjects

G Wang

Publications and source records attributed to G Wang.

At least 847 records · Page 47Linked to original sources
PubMed1990

[Prevention and treatment of emphysema in the guinea pig with ligustrazine].

This study demonstrated that ligustrazine possesses inhibitory effect obviously on elastic enzyme in vitro, and it can be used to prevent and treat emphysema instead of serum. Using light microscope and electron microscope, the authors observed morphological indexes and analyzed the indexes with stereology and statistics in lung tissues of guinea pigs. The results showed that there was no obvious difference between the ligustrazine administration group and the saline control group as pathological changes were not found in the ligustrazine administration group under microscope. The shapes of elastic proteins were the same under electron microscope observation. Aerosol inhalational method induced emphysema model of elastic enzyme in guinea pig could be improved with ligustrazine treatment.

Animals↗
PubMed1989

Progressively restricted expression of a homeo box gene within the aboral ectoderm of developing sea urchin embryos.

A homeo box-containing gene, Hbox1 is expressed in an unusual and highly conserved spatial pattern in embryos of two different species of sea urchin, Tripneustes gratilla and Strongylocentrotus purpuratus. Hybridization in situ shows that this mRNA accumulates initially throughout the aboral ectoderm; however, between blastula and pluteus stages, the region containing Hbox1 mRNA retracts gradually until only a small area around the vertex is labeled in pluteus larvae. Aboral ectoderm appears cytologically uniform and also accumulates uniform levels of other tissue-specific mRNAs. Therefore, the Hbox1 pattern reveals a previously unsuspected heterogeneity of aboral ectoderm cells and a polarity within this tissue. In S. purpuratus, the Hbox1 gene product probably is not involved in initial specification of cell fate, as this message does not achieve a significant fraction of its peak abundance until almost hatching blastula stage, well after the time aboral ectoderm cells have initiated a tissue-specific program of gene expression. RNA blot and RNase protection analyses revealed low levels of Hbox1 mRNA in all adult tissues examined. However, this message was not detectable in mature eggs, suggesting that the Hbox1 gene does not have a maternal function. In addition to highly conserved spatial and temporal patterns of expression, the homeo box genes of these two urchin species also are conserved highly in sequences outside the homeo domain, despite the divergence of these two species (30-45 my). Two notable features of the protein shared with several vertebrate homeo proteins are a short conserved sequence encoded by an exon upstream of that encoding the homeo domain and a large region of high serine and proline content.

Amino Acid Sequence↗
PubMed1989

Surgical treatment of 100 patients with W-P-W syndrome.

100 patients with W-P-W syndrome who had repeated attacks of supraventricular tachycardia (200-300 beats per minute) associated with syncope and hypotension were treated surgically. Most of them were refractory to medical treatment. Electrophysiological studies were done before operation and the anomalous conduction pathways (ACPs) were confirmed and localized by epicardial mapping during the operation. Among the 100 patients, 44 were of type B, 29 type A and 27 type A + B. The right ACPs were excised and the left ACPs were injected with alcohol. Four patients died after operation. 96 patients were followed up for an average of 24 months; 38 (90.5%) of 42 type B patients, 23 (82.1%) of 28 type A patients, and 17 (65.4%) of 26 type A + B patients were cured.

Adolescent↗
PubMed1989

Hydrocortisone promotes survival and proliferation of granulocyte-macrophage progenitors via monocytes/macrophages.

We have determined the mechanism by which hydrocortisone (Hc) promotes the survival and proliferation of normal human granulocyte-macrophage progenitors (granulocyte-macrophage colony-forming units, CFU-GM). Peripheral blood mononuclear cells were cultured in a liquid system with 0-10.0 microM Hc over 3 weeks. At 7-day intervals 50% of the culture media along with the cells (suspension cells) present in the media were removed and replaced with fresh media. No CFU-GM or very small numbers of CFU-GM were contained in the suspension cells of the 14- and 21-day-old liquid cultures without Hc; CFU-GM were present and increased with increasing concentrations of Hc. The CFU-GM content in suspension cells of 14- and 21-day-old liquid cultures with 1.0 microM Hc was at least threefold higher compared to liquid cultures without Hc. In a double-layer CFU-GM agar culture system, the suspension cells from liquid cultures with 1.0 microM Hc, but not from liquid cultures without Hc, supported CFU-GM proliferation from normal human bone marrow cells. The CFU-GM proliferation-inducing ability was confined to the monocytes/macrophages (Mo). CFU-GM colony inhibitory and stimulatory activities were detected in cell-free media recovered from liquid cultures without Hc, but only colony stimulatory activity was detected in the media from cultures with 1.0 microM Hc. These results indicate that greater than or equal to physiological concentrations of Hc (0.1-1.0 microM) are required for the persistence and proliferation of CFU-GM, and the effect of Hc is mediated through the Mo, probably by inhibiting the production of one or more of the CFU-GM colony inhibitory molecules.

Adult↗
PubMed1989

Enrichment of the spindle fiber attachments from mouse liver cell nuclei.

Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouse livers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and high speed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured by radioimmunoassay. The basic method offers opportunities for further increases of yield and for the enrichment of SFA of other vertebrates.

Animals↗
PubMed1988

Stage- and tissue-specific expression of two homeo box genes in sea urchin embryos and adults.

We report the isolation of two different homeo box genes, HB3 and HB4, from the Hawaiian sea urchin Tripneustes gratilla. DNA sequencing revealed a definitive Antennapedia (Antp) class homeo box in each gene. Southern transfer hybridizations showed the genes to be single-copy. A 5.7-kb transcript of the HB3 gene was found in ovary, testis, small intestine and gastrula poly(A)+ RNA. The HB4 gene produces three transcripts. A 3.7-kb and a 4.4-kb transcript are expressed during embryogenesis. A 3.5-kb transcript appears in each of the adult tissues studied. The HB4 gene appears to be the sea urchin cognate of the Drosophila infrabdominal-7 (iab-7) gene, the mouse Hox 1.7 and Hox 3.2 genes and the Xenopus X1Hbox 6 gene. An examination of Antp class homeo box genes in deuterostomes indicates that a chromosomal duplication has taken place in the evolutionary line leading to the vertebrates after the divergence of the echinoderms. Thus, the sea urchin represents the primitive condition.

Amino Acid Sequence↗
PubMed1988

Successful replantation in ten-digit complete amputations.

The authors report a case of successful replantation of all fingers in a ten-finger complete amputation. Among the factors responsible for the successful outcome were an adequate number of microsurgically-trained resident surgeons and staff, good organization and supervision by senior staff, emphasis on precision and perseverance in the operative procedures, and postoperative management. Follow-up is 15 months, and the patient now has a pair of functionally and cosmetically good hands.

Adult↗
PubMed1987

Anatomical analysis of the cause of skin necrosis of the great toe after transplantation of the great toe nail flap.

Necrosis of skin on the medial side of the great toe has been a significant complication of the great toe nail flap. To investigate the reason for this, a study of the blood supply of the medial side of the great toe was carried out on 55 feet with the injection of red latex into the arteries and on 13 vascular cast specimens. It was found that, after the blood vessels from the plantar and dorsal sides of the great toe are severed during the operation, the skin of the medial side of the great toe is mainly supplied by the medial vascular network at the head of the first metatarsal bone. It is clear that the blood supply from this network may sometimes be insufficient to nourish the skin. It is suggested that the operative incision should be changed so as to increase the blood supply to the medial vascular network which may decrease the incidence of skin necrosis.

Humans↗
PubMed1987

Nonoperative treatment of Hirschsprung's disease: a new approach.

The approach of the combined traditional Chinese and modern medicinal treatment of Hirschsprung's disease has been applied in 90 cases. Long-term follow-up showed satisfactory results. Of these, 55 cases with a typical history, plus a positive ACHE and x-ray were selected for analysis and discussion, making an over all rate of 78.18%. This treatment is particularly useful for those babies under 1 year of age with a short narrow segment of the rectum. In addition, it would be a good trial procedure for those babies over 1 year old with mild symptoms and the narrow segment just beyond the sigmoidorectal junction.

Acupuncture Therapy↗