Biomedical subjects

G Wang

Publications and source records attributed to G Wang.

At least 289 records · Page 16Linked to original sources

PubMed2000

Activated protein C resistance in antiphospholipid thrombosis syndrome.

OBJECTIVE: To explore the correlation between antiphospholipid antibodies (APA), activated protein C resistance (APCR) and antiphospholipid thrombosis (APL-T) syndrome and further investigate the mechanism of thrombosis in APL-T syndrome. METHODS: ELISA, PTT-LA and APTT +/- APC methods were used to detect anticardiolipin antibodies (ACA), lupus anticoagulants (LA) and APC R in 20 APL-T syndrome patients. RESULTS: Twenty patients were diagnosed as APL-T. ACA-IgG, M and LA are strongly associated with APL-T. The incidence of APCR in APL-T (75%) was significantly higher than that of the normal group (5%). CONCLUSION: There was high prevalence of APCR in APL-T, which was strongly associated with LA. It is proposed that acquired APCR induced by APA is a key to understanding thrombosis in Chinese APL-T patients.

Activated Protein C Resistance↗

PubMed2000

Expression of CDKI p27Kip1 in trophoblastic neoplasm.

OBJECTIVE: To evaluate the role of p27Kip1 in tumorigenesis and the development of trophoblastic cell disease. METHODS: Using immunohistochemistry, the expression of p27-protein was investigated in 10 normal chorionic villi in the first trimester of pregnancy, 15 complete hydatidiform moles (HM), 7 invasive moles (IM) and 7 choriocarcinomas (CC). RESULTS: In all cases, immunohistochemical staining localized p27-protein in the plasma. Decreased expression of p27Kip1 was observed in malignant trophoblastic neoplasms with a positive rate of 21.43%, which is significantly less than that in normal chorionic villi (80%) and in complete HM (73.33%) (P < 0.05). The positive rate of p27Kip1 in those complete HM with large uterine size for gestational age was lower than that in those with normal or small uterus (42.86% vs 100%, P < 0.05). CONCLUSION: p27Kip1 may be involved in the tumorigenesis of gestational trophoblastic neoplasm as a negative regulator of the cell cycle. The expression level of p27Kip1 in trophoblastic cells may be a prognostic factor for complete HM.

Cell Cycle Proteins↗

PubMed2000

Transcatheter closure of secundum atrial septal defects using Amplatzer device.

OBJECTIVE: To evaluate the feasibility and short term results of transcatheter closure of secundum atrial septal defects (TCSASDs) using Amplatzer occluder device. A new self-centering device, the Amplatzer atrial septal occluder, has recently been evaluated in an animal model with excellent results. This paper reports our clinical research with this device. METHODS: 30 patients underwent an attempt at TCSASDs at an average age of 35.6 +/- 15.9 y (range, 5-62 y) and average weight of 63.6 +/- 14.5 kg (range, 17-78 kg) using the Amplatzer atrial septal occluder. The average ASD diameter measured by transesophageal echocardiography (TEE) was 23.6 +/- 4.0 mm (range, 16-31 mm), and the average atrial septal defects (ASD) balloon stretched diameter was 25.2 +/- 5.8 mm (range, 9-34 mm). All patients had right atrial and ventricular volume overload with a Qp/Qs of 3.4 +/- 1.2 (1.4-5.2). A F8-12 catheter was used for delivery of the device in all patients. RESULTS: The device was placed correctly in all patients. There was immediate and complete closure in 30 patients. The average device waist size used to close the defect was 25.6 +/- 5.9 mm (range, 9-34 mm). There was no episode of device embolization or any other complication. Follow-up was performed using transthoracic echocardiography (TTE) 1 day, 1 month, 3 months, 6 months and 1 year after the procedure. The first day after operation; there was complete closure of the ASD in all patients; there have been no episodes of endocarditis, thromboembolism, or wire fracture. CONCLUSION: TCSASDs using the Amplatzer occluder device was an efficient nonsurgical method which had a very high technical success rate and satisfactory short term and follow-up results.

Adult↗

PubMed2000

Antigenic variation and cytoadherence of PfEMP1 of Plasmodium falciparum-infected erythrocyte from malaria patients.

OBJECTIVE: To approve a theoretical basis for the molecular pathogenesis of human cerebral malaria and treatment with prevention. METHODS: The blood samples were collected from 24 patients with cerebral malaria, 143 with falciparum malaria, 34 with vivax malaria and 20 healthy controls from the endemic areas of Yunnan Province, China. Using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technique, we determined the molecular mass (Mr) of these Plasmodium falciparum (P. falciparum) erythrocyte membrane protein 1 (PfEMP1) molecules. RESULTS: Our findings indicate that higher molecular mass (260 kDa-320 kDa) forms of PfEMP1 were expressed on parasitized erythrocyte (PE) from human cerebral malaria patients. Compared with PfEMP1 expressed on PE from human cerebral malaria patients, the expression of PfEMP1 and Plasmodium vivax (P. vivax) erythrocyte membrane protein 1 (PvEMP1) on PE from falciparum malaria patients and vivax malaria patients did not have multiple bands of PfEMP1 of > or = 260 kDa, but had a PfEMP1 with molecular mass of 240 kDa and a PvEMP1 with molecular mass of 180 kDa band separately. Healthy controls expressed an EMP of molecular mass of 140 kDa. CONCLUSION: Results confirm the antigenic variation of higher molecular mass of PfEMP1 whose molecular mass is equal to or exceeds 260 kDa-320 kDa on PE of patients with cerebral malaria. Our results show that the binding of large antigenic variability PfEMP1 molecular mass of 260 kDa-320 kDa on PE from human cerebral malaria patients with diverse receptor molecules on the endothelial cell (EC) of the cerebral microvessels may be involved in the molecular pathogenesis of cerebral malaria.

Adolescent↗

PubMed2000

[Diagnosis and treatment of colorectal non-epithelial malignancies].

OBJECTIVE: To summarize the experience in the diagnosis and treatment of colorectal malignancies of non-epithelial origin. METHODS: Seventy five cases of colorectal non-epithelial malignancy who had undergone treatment in our hospital from 1975 to 1996 were retrospectively analysed with their clinicopathologic characteristics, treatment and prognosis. RESULTS: Patients usually had no specific clinical manifectation, such as bloody purulent stool or mucous stool. No apparent mucosa damage other than changes due to pressure from without was found on x-ray and colonoscopic examinations. Diagnosis depended on pathology and clinical manifestations. Metastasis occurred mainly by hematogenous spread and local invasion. In this series, 21 cases (6 liomyosarcomas; 15 lymphosarcoma) had local recurrence. The principle treatment was operation, which was performed two or several times in patients with local recurrences. Except lymphosarcoma, the disease was not sensitive to chemotherapy or radiotherapy. The survival rate was significantly lower than that of colorectal cancers. CONCLUSION: The disease is apt to be misdiagnosed or missed because it lacks specific symptoms. Local recurrence and hematogenous metastasis are common.

Adolescent↗

PubMed2000

[Studies on 5 kinds of methods combination for detection of smear and culture negative pulmonary tuberculosis].

OBJECTIVE: To evaluate the diagnostic value of 5 kinds of methods used jointly for smear and culture negative pulmonary tuberculosis. METHODS: The high specificity methods were PCR examination for sputum TB-DNA, immunological examination (ELISA method) for serum LAM IgG, PPD IgG, SCIC and PPD 0.1 U skin test. Smear and culture positive cases were 31, smear and culture negative 54, healthy control 53, other lung disease 30. Methods were used for every case synchronously, then the sensitivity and specificity of single method and the diagnostic value of single and joint examination for smear and culture negative patients were evaluated. RESULTS: In single examination sensitivity of PCR, LAM IgG, PPD IgG, SCIC, PPD 0.1 U was 96.8%, 69.2%, 62.1%, 32.0%, 53.8% respectively; specificity was 96.2%, 98.1%, 98.1%, 98.1% correspondingly. The positive detection rate of single examination for sputum TB negative patients was just 21.4%-44.4%. In combination examination the positive rates increased remarkblely by the number combined from 2 to 5, they were 66.9%, 70.5%, 80.4%, 85.7% respectively and they were more notable than that of TB positive group. The specificity of combination examination was slightly decreased by the number combined. It decreased just by 9% and the specificity of most examination methods kept on no less than 90%. CONCLUSIONS: For diagnosis of smear and culture negative pulmonary tuberculosis combination examination using the high specificity methods can increase the sensitivity obviously and the specificity just decrease slightly. It is valuable for widely use of combination method in detection of smear and culture negative patients.

Adolescent↗

PubMed2000

[Materials fabrication of tissue engineered peripheral nerve in vitro].

OBJECTIVE: To investigate the methods to fabricate repair materials of tissue engineered peripheral nerve with bioactivity of Schwann cells (SC). METHODS: 1. The materials were made by dry-wet spinning process to fabricate PLA hollow fiber canal with external diameter of 2.3 mm, internal diameter of 1.9 mm, thickness of 0.4 mm, pore size of 20 to 40 microns, pore ratio of 70% and non-spinning fiber net with pore size of 100 to 200 microns, pore ratio of 85%. 2. SC were implanted into excellular matrix (ECM) gel to observe the growth of SC. 3. SC/ECM complex were implanted into non-spinning PLA fiber net to observe the growth of SC. 4. SC, SC/ECM and SC/ECM/PLA were implanted into PLA hollow fiber canal to bridge 10 mm defect of rat sciatic nerve. RESULTS: 1. SC were recovered bipolar shape at 1 day after implantation, and could be survived 14 days in ECM gel. 2. After SC/ECM complex was implanted into PLA net, most of SC were retained in the pore of PLA net with the formation of ECM gel. SC could be adhered and grown on PLA fiber. 3. Most of SC in ECM gel could be survived to 21 days after transplantation. Survival cell numbers of SC/ECM and SC/ECM/PLA groups were obviously higher than SC suspension group. CONCLUSION: Non-spinning PLA porous biodegradable materials with ECM is benefit for SC to be adhered and grown.

Acrylic Resins↗

PubMed2000

[The role of platelet activation in the pathogenesis of asthma].

OBJECTIVE: To investigate whether platelets are activated during an asthmatic attack and to detect the correlation between platelet activation and severity of bronchial asthma. METHODS: Plasma concentration of thromboxane B2(TXB2) and 11-dehydro-TXB2(11-DH-TXB2) and P-selectin (CD 62P) on platelet surface were measured in 44 patients with asthma and in 18 normal individuals using ELISA and FCP. The serum ECP level and pulmonary function were also analysed. RESULTS: The plasma level of 11-DH-TXB2 and TXB2 and the positive percentage of CD 62P on platelets were significantly increased in patients with acute asthma (n = 28) as compared with the control (n = 18, P < 0.01). The plasma concentration of 11-DH-TXB2 and TXB2 were decreased significanty after the control of asthma. The level of platelet activation was correlated with ECP (r = 0.785, P < 0.05), FEV1% (r = -0.867, P < 0.01) and PEF(r = -0.745, P < 0.05). CONCLUSIONS: These results suggest that there is an abnormal platelet activation in asthmatics, which can reflect the severity of bronchial asthma to a certain extent, although its exact, mechanism is unknown. The platelets secret a wide range of biologically active substances capable of inducing or augmenting the airway inflammatory responses in asthma.

Adolescent↗

PubMed2000

[Classification of secondary nasal deformities associated with unilateral cleft lip].

OBJECTIVE: To classify secondary deformities of cleft lip noses. METHODS: 56 patients with secondary nasal deformities of cleft lip were chosen at random. Cartilages of those noses were dissected and measured when correcting nasal deformity in order to find the relationship between anatomical anomaly of nasal cartilages and nasal deformity. RESULTS: Three grades of anatomical anomalies of the nasal framework were found. Grade I, the morphological anomaly occurs at lower third of the diseased side only. Grade II, the lower third of the another side is involved in addition. Grade III, the dorsum nasi is also deviated. CONCLUSIONS: This classification may be employed as a practical guide for surgical planning.

Adolescent↗

PubMed2000

[Study of congenital velopharyngeal insufficiency and hypernasality].

OBJECTIVE: To evaluate 27 congenital veloparyngeal insufficiency(CVPI) subjects and 6 normal articulation subjects with airflow pressure techniques. METHODS: A compare analysis was performed in two groups (CVPI subjects and normal articulation subjects) during speech production of /hemper/ and /papa/. RESULTS: There wasn't statistical significance between two groups in oral air pressure and velopharyngeal orifice areas, however, CVPI group differed significant in nasal airflow on the articulation of /hemper/ and /papa/. CONCLUSIONS: When the degree of CVPI subject's VP opening was small, hypernasality appeared to be related to duration of the opening-closing movements.

Adolescent↗

PubMed2000

[An experimental study on F-heparin surface modified IOLs implanted into the Rhesus monkeys' eyes].

OBJECTIVE: To evaluate the biocompatibility of F-heparin surface modified IOLs. METHODS: 13 monkeys were divided into 3 groups, and implanted with different surface modified and non-modified IOLs into their eyes. All of the eyes were examined by slitlamp microscope and Schiötz tonometer at postoperative 15, 30, 60, 90 and 180 days. Postoperatively, at different periods the aqueous was aspirated from the anterior chamber to calculate cells. RESULTS: F-heparin surface modified IOLs induced milder inflammatory reaction and less dense posterior capsula opacification (PCO) than non-modified IOLs. CONCLUSION: F-heparin surface modified IOL has a better biocompatibility.

Animals↗

PubMed2000

[Spectrum of mycotic keratitis in China].

OBJECTIVE: To investigate the spectrum of mycotic keratitis in China. METHODS: We scraped and evaluated 615 mycotic keratitis in microbiology laboratories of Zhengzhou (central China), Shijiazhuang (north China) and Guangzhou (south China) in the twenty-two years between Jan. 1, 1975 and Jun. 30, 1997. RESULTS: Organisms of 18 genuses and 615 species isolated from corneal ulcers were found. Fusarium species were isolated the most frequently (65.0% in Zhengzhou, 33.3% in Shijiazhuang and 39.2% in Guangzhou), followed by Aspergillus (20.5% in Zhengzhou, 21.7% in Shijiazhuang, 30.7% in Guangzhou), Penicillium (3.6% in Zhengzhou, 11.6% in Shijiazhuang) and Curvularis (13.1% in Guangzhou). Between Jan. 1, 1975 and Dec. 31, 1986, Aspergillus species were most commonly isolated in Guangzhou (48.5%), followed by Fusarium (27.7%) and Penicillium (14.27%). CONCLUSIONS: Just as different regions of the world are characterized by specific endemic infections, the predominating organisms in corneal fungal infections also vary throughout the world. These regional differences in causative organisms in mycotic keratitis are clinically important because they influence the initial and more definite therapy. This study shows that Fusarium species are the most common organisms of mycotic keratitis in China.

Aspergillus↗

PubMed2000

[Homogeneity study on the Streptococcus suis isolated from human and swine].

OBJECTIVE: To identify S. suis and to evaluate the homogeneity of isolates of S. suis from human and swine. METHODS: Culture, morphology, API biochemical tests and serum coagulate tests were used. All the 7 strains of streptococcus were confirmed to be S. suis serotype 2. Two strains and 1 strain were isolated from blood and cerebrospinal fluid (CSF) of patients, respectively, while the other 4 strains were obtained from the infected swine or their corpses. Seven strains of S. suis serotype 2 and reference strain SS2 were analyzed by randomly amplified polymorphic DNA analysis with six primers and Rep PCR with four primers and thalli fatty acid profile analysis. Cluster and principal compound analysis of results were performed with RAPD, Phylip and Treeview software. RESULTS: By analysis on RAPD patterns, a close relationship among 7 strains of S. suis serotype 2 and reference strain SS2 was discovered. Human-born strains and swine-born strains exhibit similar RAPD patterns. There was a same clonal relationship between strains originated from the blood and CFS of the patients. These results were confirmed by thalli fatty acid profile to have genotypic and phenotypic identity. CONCLUSION: Homogeneity exists among the 7 strains and reference strain SS2.

Animals↗

PubMed2000

[The effect of fluoride-arsenic exposure on the lipid peroxidation and antioxidation of the offspring of rats].

OBJECTIVE: To provide information on the effects of the offspring of rats exposed to fluoride-arsenic. METHODS: The levels of lipid peroxidation and the abilities of antioxidation were determined in the blood of the rats and their offsprings under two generations-one nest reproductive test. RESULTS: The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the blood decreased with the increase of exposure dose. For example, the activity of SOD, was 14.56, 13.74, 11.89 and 11.21 micromol x min(-1) x mg Hb(-1) in different dose groups of F(2), respectively. In contrast, the concentration of lipid peroxides (LPO) increased. Eight weeks after exposure, the activities of SOD and GSH-Px increased, the activity of SOD was 13.97, 13.55, 13.47 and 12.76 micromol x min(-1) x mg Hb(-1), respectively, and the concentration of LPO returned to normal level. CONCLUSION: Fluoride-arsenic exposure can cause oxidative damage of the rat's offspring.

Animals↗

PubMed2000

[Studies on human streptococcal infectious syndrome caused by infected pigs].

OBJECTIVES: To demonstrate the clinical manifestations of S. suis infectious syndrome; to study the characteristics of causative organism and its source. METHODS: 25 cases of unknown causes of food poisoning were identified in the central area of Jiangsu province, China in July 1998. Biological specimens was collected in some patients, and the causative organism was isolated using a number of different culture medium. Isolated organisms were identified by serum antibody tests, API biochemical tests, drug sensitivity tests and animal model experiments. The human born strains and the pig born strains were compared by RAPD technique. Epidemiological methods were applied to trace the source of causative organisms. RESULTS: Cases were clinically categorized into two types: Streptococcal toxic shock syndrome (STSS) and streptococcal meningitis syndrome (SMS). Three strains of streptococci were isolated respectively from the blood and cerebrospinal fluid of 6 cases. All these 6 strains were confirmed to be S. suis type 2 by culture characteristics, morphology, biochemical characteristics, serum antibody tests and fatty acid profile analysis. Animal model experiment showed that these strains were sensitive to rabbits and pigs, but not to rats. RAPD fingerprint test revealed an identity between human born and pig born strains. CONCLUSIONS: The aetiological agent of these human cases was S. suis type 2. Human cases were contracted by direct contact with the infected pigs or the corpse of infected pigs.

Adult↗

PubMed2000

[Relationship between blood lipid level and apolipoprotein E gene polymorphism in offspring from longevity and non-longevity families].

OBJECTIVE: To study relationship between genetic factor and level of blood lipid. METHODS: Levels of blood lipid and difference in apolipoprotein E (apo-E) allele frequencies were compared in 86 offspring in seven Uigur longevity families and 62 offspring in nine Uigur non-longevity families and their relationship was explored. RESULTS: After adjustment for age and occupation, blood indicators related to atherosclerosis were all lower in the offspring of longevity families than in non-longevity families, and those correlated inversely with it were higher in the former. Blood levels of triglyceride and apo-B were significant lower (P < 0.05) and the ratio of HDL-ch to LDL-ch was significantly higher (P < 0.05) in male offspring of longevity families than of non-longevity families. The ratio of apo-A to apo-B was significantly higher in female offspring of longevity families than of non-longevity families. Frequency of epsilon4 allele also was significantly fewer in the offspring of longevity families than of non-longevity ones (P < 0.01). Also, analysis showed that individuals with epsilon4 allele had higher levels of serum total cholesterol, lower density lipoprotein cholesterol and apo-B than those with other alleles. CONCLUSION: Average level of blood lipid was better in the offspring of longevity families than that of non-longevity families. apo-E allele polymorphism can in part explain its difference.

Adolescent↗

PubMed2000

[Myocardial apoptosis induced by delayed fluid resuscitation in a burned rat model].

OBJECTIVE: To explore the possibility and the mechanism of myocardial apoptosis induced by delayed fluid resuscitation in a burned rat model and its relationship with nitric oxide (NO) and oxygen-derived free radicals. METHODS: In a rat model with 30% III degree burn, the genomic DNA of the myocardial tissue was detected with ApoAlert(TMLM)-PCR Ladder Assay Kit and visualized with agarose gel electrophoresis. Meanwhile, the NO and the content of unsaturated fatty acids were measured. RESULTS: In rats receiving delayed fluid resuscitation following burn, the myocardial genomic DNA exhibited DNA ladder-index of apoptosis, and the contents of myocardial NO and unsaturated fatty acid were much lower than those in rats receiving immediate resuscitation (P < 0.05). CONCLUSION: The myocardial tissue undergoes apoptosis in burned rats receiving delayed fluid resuscitation, and the decreased NO and the production of oxygen-derived free radicals are also observed in this process.

Animals↗

PubMed2000

[Antiphospholipid thrombosis syndrome and acquired activated protein C resistance].

OBJECTIVE: To explore the correlation between antiphospholipid antibodies (APA) activated protein C resistance (APCR) and antiphospholipid thrombosis (APL-T) syndrome and investigate the mechanism of thrombosis in APL-T syndrome. METHODS: ELISA, APTT-LA and APTT+/-APC methods were used to detect anticardiolipin antibodies (ACA), lupus anticoagulants (LA) and APCR in 20 APL-T syndrome patients respectively. RESULTS: Twenty patients were diagnosed as APL-T. The patients were classified in three ways. According to etiology, 14 of 100 systemic lupus erythematosus (SLE) patients were diagnosed as "secondary" APL-T, and 6 of 16 "unexplained" thrombosis and spontaneous abortion as "Primary" APL-T syndrome. According to antibodies, 12 of 20 patients with positive LA were diagnosed as lupus anticoagulant thrombosis syndrome (LA-T), 12 of 20 patients with positive ACA as anticardiolipin antibodies thrombosis syndrome (ACA-T). According to clinical presentation: 7 of 20 patients with deep vein thrombosis were classified as type I APL-T, 3 of 20 patients with cerebrovascular thrombosis as type II, 1 of 20 patients with vein cerebrovascular thrombosis and spontaneous abortion as type IV (mixing), and 9 of 20 patients with spontaneous abortion as type V (fetal wastage type). The ACAs and LA are strongly associated with APL-T. There is close relationship between APCR and APL-T. CONCLUSION: The acquired APCR might be an important reason for thrombosis in APL-T caused by APA and LA.

Activated Protein C Resistance↗