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Biomedical subjects

G Wagner

Publications and source records attributed to G Wagner.

At least 667 records · Page 37Linked to original sources
PubMed1977

[The prompt squatting in the ambulance of pediatric cardiology (author's transl)].

The result of prompt squatting is an increase of the venous return and of the arterial resistance. Consequences are increasing of the end-diastolic and systolic chamber pressure, stroke volume, average arterial blood pressure as well as decreasing of the frequency of the pulse controlled by baro-receptors. The regulatory mechanism both change the intensity of heart murmurs of various cardiopathies and of functional heart murmurs in children. Only the systolic murmur of muscular subvalvular aortic stenosis softens. The experiment is most valuable for the recognition of mild insufficiency of semilunar valves.

Adolescent↗
PubMed1977

[Antenatal diagnosis of a foetal heart block in the 33. week of pregnancy (author's transl)].

In a pregnant woman of 20 years is recorded a foetal bradycardia of 60 beats per minute in the 33. week of pregnancy. The woman too had an influenza. The cardiological examination shows a congenital complete heart block. After spontaneous delivery of a male infant at date the heart block persists for more than 7 months. There are are no malformations; a interdependence with influenza-infection of the mother is not to be proved. The diagnosis of the congenital heart block is rare. Of 40 cases pointed out in the literature only 3 cases has been diagnosed before the 35. week of pregnancy.

Adult↗
PubMed1976

Dynamics of the aromatic amino acid residues in the globular conformation of the basic pancreatic trypsin inhibitor (BPTI). I. 1H NMR studies.

The basic pancreatic trypsin inhibitor (BPTI) was investigated by high resolution 1H NMR techniques at 360 MHz. Observation of the amide proton resonances of the polypeptide backbone showed that the globular conformation of BPTI determined by X-ray studies in single crystals is maintained in aqueous solution over the temperature range from 4 degrees to 87 degrees. NMR studies over this temperature range of the aromatic amino acid residues of BPTI. i.e. 4 tyrosines and 4 phenylalanines, led to complete assignments of all the aromatic spin systems in the protein. From this, information was obtained on the rotational motions about the C beta--Cv bond axis of the aromatic rings in the globular form of PBTI. At 25 degrees, two tyrosine rings and one phenylalanine ring are rotating rapidly on the NMR time scale. For the other rings the transitions from slow to rapid rotational motions were investigated at variable temperatures and energy barriers for these intramolecular rate processes determined. The studies of the tyrosine resonances had been described in detail in a previous publication. The present paper describes the identification of the phenylalanine resonances and comments on some technical aspects which might be of quite general interest for the analysis of highly resolved 1H NMR spectra of proteins. Data for the tyrosines and the phenylalanines are compiled in three tables, i.e. the pK alpha-values for the tyrosines, the NMR parameters for all eight aromatics, and the parameters delta G not equal to, and, where available, delta H not equal to and delta S not equal to for the rotational motions of the rings.

Amino Acids↗
PubMed1976

Analysis of the structure of cancer literature based on SABIR-C. III. A survey of the distribution of the world oncological publications from 1969-1974.

The great number of disciplines involving oncology and the exponential growth in the number of publications make it increasingly more difficult to keep abreast of the important oncological literature appearing in a large number of periodicals. It is, therefore, essential for the oncologist to be up to date with the journals and to read the papers relevant to his specialty. The cancer literature information system SABIR-C developed by the French and German Cancer Research Centers was created for this purpose. The present paper analyses 54496 papers published in periodicals covered by the SABIR-C system in the period from January 1969 to October 1974. The tables included contain the titles of periodicals which are of particular relevance for cancer research. They permit a quick orientation with regard to the important sources of oncological publications. These tables also assist the librarian working in a cancer research institution in selecting the most important and quantitatively most productive periodicals. This is especially important since he usually has only limited financial resources at his disposal. Further tables give a survey of the countries of origin and the categories to which the latest oncological literature belongs.

Documentation↗
PubMed1976

A study of the lysyl residues in the basic pancreatic trypsin inhibitor using 1H nuclear magnetic resonance at 360 Mhz.

Fourier transform 1H nuclear magnetic resonance (NMR) experiments at 360 MHz using convolution difference techniques to improve the spectral resolution were employed to investigate the resonances of the lysyl residues in bovine pancreatic trypsin inhibitor. The observations in both native protein and in chemically modified protein containing Nepsilon-dimethyllsysine show that three of the four lysines extend predominantly freely into the solvent, whereas lysine-41 is involved in an intramolecular interaction with tyrosine-10. Since in the single crystal structure tyrosine-10 is involved in an intermolecular interaction with arginine-42 of the neighboring protein molecule, the NMR data thus reveal a local conformation difference for bovine pancreatic trypsin inhibitor in solution and in the crystalline form which appears to result primarily from intermolecular interaction in the crystal lattice.

Hydrogen-Ion Concentration↗
PubMed1976

Uronic acid dehydrogenase from Pseudomonas syringae. Purification and properties.

1. Uronic acid dehydrogenase was purified to homogeneity. After a 338-fold purification a yield of 16% was achieved with a specific activity of 81 mumol NADH formed min-1 mg protein-1. 2. The purity of the enzyme was controlled by disc electrophoresis, sodium dodecylsulfate electrophoresis and ultracentrifugation. 3. A molecular weight of 60 000 was determined by gel chromatography and by ultracentrifugation. 4. The native enzyme is composed of two subunits, their molecular weight being 30 000 as estimated by sodium dodecylsulfate electrophoresis. The subunits as such are inactive. 5. The absorption spectrum with a maximum at 278 nm shows no evidence for a prosthetic group. 6. For catalytic activity no SH groups and no metals seem to be necessary. 7. The Michaelis constants determined with the pure enzyme are for glucuronic acid Km = 0.37 mM, galacturonic acid Km = 54 muM and NAD+ (with glucuronic acid) Km = 80 muM. 8. A weak reverse reaction could be observed with glucaric acid lactones at acidic pH. 9. NADH is competitive with NAD+. The inhibitor constant is Ki = 60 muM. 10. The NAD+ binding site seems to be of lower specificity than the uronic acid binding site.

Aldehyde Oxidoreductases↗
PubMed1976

A new enzymatic method for the determination of free and conjugated glucuronic acid.

A new method is reported for the quantitative determination of glucuronic and galacturonic acid, which is based on spectrophotometric measurement of NADH. The NAD-linked oxidation of the uronic acids to the corresponding dicarboxylic acids is measured in the presence of uronic acid dehydrogenase. This enzyme was isolated from Pseudomonas syringae. The test is highly specific for glucuronic and galacturonic acid and permits the exact determination of free and conjugated glucuronic acid. This enzymatic determination of glucuronic is the most sensitive method available today.

Aldehyde Oxidoreductases↗