Biomedical subjects
G Wagenaar
Publications and source records attributed to G Wagenaar.
High expression of the HMG box factor sox-13 in arterial walls during embryonic development.
Members of the Sox gene family of transcription factors are defined by the presence of an 80 amino acid homology domain, the High Mobility Group (HMG) box. Here we report the cloning and initial analysis of murine Sox-13 . The 984 amino acids Sox-13 protein contains a single HMG box, a leucine zipper motif and a glutamine-rich stretch. These characteristics are shared with another member of the Sox gene family, Sox-6. High level embryonic expression of Sox-13 occurs uniquely in the arterial walls of 13.5 days post coitum (dpc) mice and later. Low level expression was observed in the inner ear of 13.5 dpc mice and in a limited number of cells in the thymus of 16.5 dpc mice, from which Sox-13 was originally cloned. At 18.5 dpc, Sox-13 is expressed in the tracheal epithelium below the vocal cord and in the hair follicles. The Sox-13 protein binds to the consensus HMG box motif, AACAAAG, but does not transactivate transcription through a concatamer of this motif. Sox-13, like other members of the Sox family likely plays an important role in development.
Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
Tcf transcription factors interact with beta-catenin and Armadillo to mediate Wnt/Wingless signaling. We now report the characterization of genes encoding two murine members of the Tcf family, mTcf-3 and mTcf-4. mTcf-3 mRNA is ubiquitously present in embryonic day 6.5 (E6.5) mouse embryos but gradually disappears over the next 3 to 4 days. mTcf-4 expression occurs first at E10.5 and is restricted to di- and mesencephalon and the intestinal epithelium during embryogenesis. The mTcf-3 and mTcf-4 proteins bind a canonical Tcf DNA motif and can complex with the transcriptional coactivator beta-catenin. Overexpression of Wnt-1 in a mammary epithelial cell line leads to the formation of a nuclear complex between beta-catenin and Tcf proteins and to Tcf reporter gene transcription. These data demonstrate a direct link between Wnt stimulation and beta-catenin/Tcf transcriptional activation and imply a role for mTcf-3 and -4 in early Wnt-driven developmental decisions in the mouse embryo.
[The veterinarian versus intensive livestock husbandry. An interview with Professor G. Wagenaar].
Explore the source record for details and available documents.
[The future of the veterinarian, with particular reference to the number of veterinary surgeons (author's transl)].
Two reports on the prospects for the future of the veterinarian served as the basis for an estimate of requirements up to 2000. Much more veterinarians have so far been placed than was believed to be possible. However, one of the reasons for which this could be done, was the considerable increase of the livestock population. In the present conditions, it would appear warranted not to overestimate the prospects up to the year 2000.
[Genetics and latent defects in animals. Microphthalmia in sheep (author's transl)].
A sheep farmer purchased ten Texel ewes from a sheep breeder. He bought a ram from another sheep breeder. The ewes gave birth to twenty lambs. Seven lambs born of six ewes showed the features of microphthalmia. This is a simple recessive heritable defect. The buyer demanded annulment of the purchase by virtue of Section 1540 of the Civil Code. The court in Middelburg gave judgement for the plaintiff regarding the six ewes which had given birth to the lambs, showing the defect. On appeal, however, the court of justice in The Hague decided the cases in favour of the vendor, experts having defined the term 'breeding ewe' and a number of others having answered questions.
[Incidence of Clostridium botulinum in the rumen contents and faeces of cattle fed brewers' grains naturally contaminated with Clostridium botulinum (author's transl)].
The number of Clostridium botulinum type B organisms excreted by cattle fed brewers' grains in which these organisms were found to be present and the period for which they were excreted, were studied. Large numbers (10(5) - 10(7) per gramme) of these organisms were detected in the rumen contents and faeces of the animals. When feeding brewers' grains was discontinued, Cl. botulinum type B was still detectable in the faeces for a considerable period (greater than eight weeks). There was evidence to suggest that the number of Cl. botulinum organisms multiplies in the gastrointestinal tract of cattle.
[The purchaser's veterinarian and latent defects (author's transl)].
When the purchaser of an animal is dissatisfied with the animal bought, the question may arise whether the animal has any latent defect. The purchaser's veterinarian should see to it that he retains the initiative in this matter so that he can calmly and carefully decide whether a certificate of latent defect can or cannot be issued. The various possibilities are reviewed.
[Food poisoning in cattle caused by ingestion of brewers' grains contaminated with Clostridium botulinum type B (author's transl)].
In 1977, outbreaks of disease associated with serious losses occurred on twenty cattle farms. This disease was due to the fact that the animals had been fed brewers' grains. The clinical picture was marked by anorexia, profuse salivation, regurgitation and dehydration. The course of the disease varied with the severity of the attack. Toxicological tests were negative. Cl. botulinum type B as well as toxin type B were found to be present in the brewers' grains fairly often. As a rule, Cl. botulinum was also detected in the rumen contents and faeces. The treatment of the animals is described; this resulted in recovery in a number of cases. When experimental animals were fed brewers' grains from farms on which outbreaks had occurred, this induced similar clinical pictures. The results of studies on the aetiology and pathogenesis are discussed. It is concluded that the clinical picture was an atypical form of botulism caused by Cl. botulinum type B. Possible causes of this abnormal clinical picture are discussed in greater detail.
[The seller's veterinary surgeon and the hidden faults in animals (author's transl)].
Explore the source record for details and available documents.
[Iron Dextran Administered to Horses (author's transl)].
A description is given of three cases in which horses died very shortly after being given an intramuscular injection of iron dextran. The use of iron dextran in the case of horses is inadvisable.
[The number of veterinary surgeons today and in 1980 (author's transl)].
Explore the source record for details and available documents.
Isolation of a non-haemadsorbing strain of African swine fever (ASF) virus from a natural outbreak of the disease.
Explore the source record for details and available documents.
[Haematuria! (author's transl)].
Explore the source record for details and available documents.