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Biomedical subjects

G W Miller

Publications and source records attributed to G W Miller.

At least 19 recordsLinked to original sources
PubMed1995

Immunocytochemical detection of tumor cells in bone marrow and peripheral blood stem cell collections from patients with ovarian cancer.

High-dose chemotherapy (HDC) followed by autologous hematopoietic reconstitution is an experimental treatment option for patients with epithelial ovarian cancer. However, the incidence of occult ovarian tumor cell involvement in autologous bone marrow (BM) or peripheral blood stem cell (PBSC) autografts has not been widely investigated. We used a highly sensitive immunocytochemical (ICC) procedure that detects occult blood-borne tumor micrometastases. We analyzed 24 BM specimens (15 obtained during therapy and 9 harvest samples) and seven PBSC specimens from 22 patients with ovarian cancer. Overall, ICC analysis detected immunostained tumor cells in 10 of 23 evaluable BM specimens (43%) from 9 of 19 patients (47%). One of 9 (11%) harvest samples contained tumor cells. Only one of the 10 ICC-positive BM specimens had tumor cells detected by routine histopathological analysis. ICC-detectable tumor cells were cleared from the marrow of two patients during chemotherapy. None of the seven PBSC specimens contained tumor cells. We conclude that ovarian cancer micrometastases have the potential to contaminate BM, as is also the case in patients with other epithelial malignancies. In the limited number of specimens analyzed, PBSC harvests appeared to provide a less tumor-contaminated source of hematopoietic stem cells for autologous transplantation.

Antibodies, Monoclonal↗
PubMed1992

Evidence of a neurogenic component during IgE-mediated inflammation in mouse skin.

IgE-mediated inflammation was measured in mouse footpads that lacked sciatic innervation. Mice were passively sensitized with a monoclonal antibody, IgE anti-dinitrophenol, or were immunized for specific IgE production. Antigen-induced swelling in the denervated footpads was reduced 23-39% when compared to sham or untreated controls. Reduced IgE-mediated swelling responses were attributed to the loss of a mast cell-nerve interaction and not to blood vessel sensitivity to vasoamines. Furthermore, electrical stimulation of the distal segment of the sciatic nerve completely restored IgE-mediated inflammation. These data provide in vivo evidence that peripheral nerves participate in cutaneous IgE-mediated swelling reactions with the net effect of increasing inflammation.

Analysis of Variance↗
PubMed1992

Effects of phosphate loading on metabolic and myocardial responses to maximal and endurance exercise.

Six trained male cyclists and triathletes participated in a double blind study to determine the effects of phosphate loading on maximal and endurance exercise performance. Subjects ingested either 1 gm of tribasic sodium phosphate or a glucose placebo four times daily for 3 days prior to performing either an incremental maximal cycling test or a simulated 40-km time trial on a computerized race simulator. They continued the supplementation protocol for an additional day and then performed the remaining maximal or performance exercise test. Subjects observed a 17-day washout period between testing sessions and repeated the experiment with the alternate supplement regimen in identical fashion. Metabolic data were collected at 15-sec intervals while venous blood samples and 2D-echocardiographic data were collected during each stage of exercise during the maximal exercise test and at 8-km intervals during the 40-km time trial. Results indicate that phosphate loading attenuated anaerobic threshold, increased myocardial ejection fraction and fractional shortening, increased maximal oxidative capacity, and enhanced endurance performance in competitive cyclists and triathletes.

Adult↗
PubMed1990

Effects of phosphate loading on oxygen uptake, ventilatory anaerobic threshold, and run performance.

Seven male competitive runners (VO2max 73.9 +/- 6.3 ml.kg-1.min-1) participated in a two-session, placebo, double-blind study to determine the effects of phosphate loading on oxygen uptake, ventilatory anaerobic threshold, and 5-mile run performance. Subjects ingested 1000 mg of tribasic sodium phosphate or a placebo four times daily for 6 d. A maximal running stress test or a 5-mile performance run was performed randomly on either the 3rd or the 6th d. Test sessions were separated by a 2-wk washout period and repeated with alternating phosphate and placebo regimens. Venous blood samples were collected prior to and following each max and run session. Results revealed that placebo resting serum phosphate levels were mildly elevated and that phosphate loading significantly increased resting and post-exercise serum phosphate values. Resting and post-exercise 2,3-diphosphoglycerate values were decreased while hemoglobin values were elevated with phosphate ingestion. Phosphate loading significantly increased maximal oxygen uptake from 4.77 +/- 0.29 to 5.18 +/- 0.25 l.min-1 and ventilatory anaerobic threshold from 3.74 +/- 0.28 to 4.18 +/- 0.14 l.min-1. Five-mile run times were nonsignificantly different between placebo and phosphate sessions. However, mean performance run oxygen uptake was significantly lower (3.87 +/- 0.3 to 3.80 +/- 0.3 l.min-1) with phosphate ingestion. Data demonstrate that maximal and run performance were influenced by elevations in serum phosphate eliciting an increased maximal oxygen uptake, ventilatory anaerobic threshold, and variable effects on 5-mile run performance. These adaptations occurred without observable increases in red cell 2,3-diphosphoglycerate.

Anaerobic Threshold↗
PubMed1988

Pneumolabyrinth as a late sequela of temporal bone fracture.

Pneumolabyrinth, air in the labyrinthine system, is an uncommon entity. We report one case, adding to previous reports of two such cases. The diagnosis was made radiologically, which has become possible only recently with the advent of high-resolution computed tomography (CT) scanners; we suspect it will be used more often in the future. To our knowledge, this is the first case in which pneumolabyrinth has been identified as a delayed sequela of a temporal bone fracture. Furthermore, this is the first description of a pneumolabyrinth occurring in a transverse temporal bone fracture.

Humans↗
PubMed1987

Cochlear implant: audiometric results with a single-channel device.

With the FDA approval of the first cochlear implant device in November 1984, the possibility of the perception of sound became a reality for the profoundly deaf individual. The cochlear implant group of Cincinnati was involved in the coinvestigator study before the approval of the device. This paper presents data from seven patients with cochlear implants and the resulting improvement in their hearing perception, as well as a discussion about which patients can benefit from the cochlear implant.

Adult↗
PubMed1986

Denominator definition by the utilization correction factor method.

Most clinical research in the United States has been carried out in atypical populations. This study, done in a network of primary care populations in Virginia, calculates the denominator by the utilization correction factor method and compares the demography with that of the population of the state. The demographic characteristics of the patients in the network are very similar to those of the underlying populations and on the same order of state-of-the-art sampling methods in current use.

Adolescent↗
PubMed1986

Rumen microbial changes in cattle fed diets with or without salinomycin.

Four rumen-fistulated steers, randomly assigned to two groups (control and salinomycin fed) were used to monitor the changes in rumen microbial populations and volatile fatty acids (VFA) concentrations associated with feeding salinomycin (0.22 mg X kg-1 X day-1). Steers were adapted to an alfalfa hay and grain (80:20) diet before supplementing the diet with salinomycin, and then the diet was changed to 50:50 and 20:80 ratios of alfalfa hay to grain at 2-week intervals. Rumen samples for total and selective enumeration of anaerobic bacteria. VFA analysis, and enumeration of protozoa were collected during the 80:20 alfalfa hay-to-grain diet before salinomycin feeding, and during the 80:20, 50:50, and 20:80 hay-to-grain diets with salinomycin. At each sampling period, rumen samples were collected at 3 h after feeding on three consecutive days. Salinomycin feeding had no effect on rumen pH and total VFA concentration. The acetate-to-propionate ratio was significantly lower in salinomycin-fed steers than in the control. The molar proportion of butyrate increased in both control and salinomycin-fed steers. Total anaerobic bacterial counts were lower in salinomycin-fed steers than in the control steers after 8 weeks of salinomycin feeding. Salinomycin-resistant bacteria increased from 7.6 to 15.6% in salinomycin-fed steers but remained unchanged in control steers. Salinomycin had no effect on cellulolytic and lactate-utilizing bacteria, but the proportion of amylolytic bacteria was higher in salinomycin-fed steers than in control steers. The total number of protozoa decreased initially in salinomycin-fed steers. The initial reduction was due to reduced numbers of Entodinium species. Holotrichs were unaffected by salinomycin feeding.

Animal Feed↗
PubMed1986

Use of the neodymium/yttrium aluminum garnet laser in middle ear surgery: a preliminary report.

Clinical usefulness of the neodymium/yttrium aluminum garnet (Nd:YAG) laser in surgery of the middle ear is unproven. An animal study was designed to examine the influence of Nd:YAG laser irradiation on cochlear potentials following vaporization of the stapes. Electrocochleagraphic changes were observed in two of the five animals studied, and caution is advised before using this instrument in humans.

Animals↗
PubMed1985

Perilymph fistula.

Perilymph fistula occurs when endolymph and perilymph mix, or when perilymph leaks into the middle ear space. Sensorineural hearing loss and/or vertigo may result. This paper reviews the pertinent anatomy and physiology of the inner ear, clinical presentations, diagnosis, treatment, and prognosis as a guide for the clinician.

Adult↗
PubMed1985

The cocaine habit.

Although cocaine is considered "safe" by many drug abusers, serious psychosocial problems and medical complications, including death, can occur as the direct result of addiction to this drug. The mainstays of therapy include motivation, meticulous physical and laboratory evaluation, abstinence, and support during withdrawal. Diazepam and propranolol drugs are useful during this difficult period. Several commonly used psychotropic drugs are contraindicated. Supportive care and counseling are helpful, but a relapse is probable.

Adult↗
PubMed1984

Malignant external otitis.

Malignant external otitis (MEO) is a potentially life-threatening Pseudomonas infection of the external auditory canal in which rapid diagnosis is the key to proper treatment. We present an anatomic description of the ear and skull base, with a review of the literature on MEO, in the hope that it will enable primary care physicians to work more closely with otolaryngologists in dealing with this illness.

Aged↗
PubMed1984

Principles of alcohol detoxification.

The family physician serves as the physician of first contact for the alcoholic entering an inpatient detoxification facility. Abstinence is essential for recovery. The patient must be observed for evidence of the withdrawal syndrome. This is treated with supportive care, mild sedation, and mineral and vitamin replacement. The postwithdrawal period involves educating the patient about his illness, counseling him and his family, and planning his discharge.

Alcoholism↗
PubMed1983

Intratemporal schwannoma of the facial nerve.

Two patients with schwannomas that originated from the region of the geniculate ganglion of the facial nerve are reported. They presented with progressive facial nerve paralysis and variable disturbance of hearing. Computed tomograms demonstrated a mass lesion eroding the temporal bone (petrous portion) and extending into the middle fossa. The treatment consisted of complete excision of the tumor, and a hypoglossal-facial nerve anastomosis. Computed tomography should be performed on all patients with facial palsy of a progressive nature. Early diagnosis and surgical treatment of facial schwannoma should permit preservation of function of the facial and the acoustic nerves.

Adult↗
PubMed1983

Surgical management of vestibular Meniere's disease with endolymphatic mastoid shunt.

The early stages of Meniere's disease may consist only of vestibular symptoms. These patients with vertigo alone may be incapacitated by this condition. A great many of these patients are unresponsive to medical therapy and tend to progress to cochleovestibular disease. Early surgery on such individuals may decrease vertigo and give the added benefit of preserving hearing. This paper reports the results of endolymphatic mastoid shunts performed on 24 patients with normal hearing who had medically uncontrolled vestibular Meniere's disease. The patients underwent surgery between 1978 and 1981, and each has been followed for at least 1 year. Vertigo was decreased or abolished in 21 of 24 patients (87%). Hearing remained unchanged in 19 of the 24 patients (79%) and was mildly impaired postoperatively in 4(16.7%). Only 1 patient had moderate postoperative hearing loss. Of the 5 patients with reduced hearing, 4 had improvement of vertigo. There were no cases of surgically-induced deafness.

Adult↗