Biomedical subjects

G W Long

Publications and source records attributed to G W Long.

57 records · Page 4Linked to original sources

PubMedDate not supplied

QBC and Giemsa-stained thick blood films: diagnostic performance of laboratory technologists.

This study compared the proficiency of military medical technicians in the diagnosis of malaria using Giemsa-stained thick blood films (GSF) and the QBC system. Fourteen technicians, with no previous experience of the QBC and limited experience in interpreting GSF, were given training in the 2 techniques and then tested on their ability to make a malaria diagnosis when provided with previously prepared GSF and QBC specimens (9 positive and 10 negative). The students achieved a sensitivity of 75% and 84%, respectively, with the QBC and GSF; specificity was 84% and 76%. There was no difference in the concordance of the 2 techniques with the actual diagnosis (80% vs. 81%).

Adult↗

PubMedDate not supplied

Subchronic oral toxicity of tributoxyethyl phosphate in the Sprague-Dawley rat.

The effects of the widely used alkyl phosphate, tributoxyethyl phosphate (TBOP), were investigated in male and female Sprague-Dawley rats. This chemical was administered (low dose: 0.25 ml/kg X day; high dose: 0.50 ml/kg X day) by gavage once a day for 5 days/wk, over a period of 18 wk. Histopathological examination of tissues in treated male rats showed the presence of cardiac lesions, including myocardial necrosis with inflammatory cell infiltration. This study indicated that oral administration of TBOP may have caused or contributed to the early onset of a common background finding in this rat strain.

Administration, Oral↗

PubMedDate not supplied

Tissue eosinophilia in human coccidioidomycosis.

Eosinophilia in peripheral blood and tissue of patients with coccidioidomyocsis was reviewed in an examination of the importance of this cellular response to Coccidioides immitis. Peripheral blood eosinophilia (greater than 5%) was found in each of two patients with primary infection but was markedly more pronounced (27%-53%) in three of five patients with disseminated coccidioidomycosis. Unusually large numbers of tissue eosinophils and organized microabscesses containing eosinophils were found in six patients with progressive or disseminated disease. These six patients all demonstrated cutaneous anergy to coccidioidin. Eosinophilic microabscesses were found surrounding rupturing spherules and free endospores. Phagocytosis of free endospores was not demonstrated by the technique used. The bone marrow of two patients with disseminated disease showed a marked increase in eosinophilic myeloid precursors; this result suggested the presence of an eosinophilopoietin. Although peripheral blood eosinophilia has been explained as a hypersensitivity response to C. immitis that represents a favorable prognosis, the data reported herein suggest that peripheral blood eosinophilia of greater than 20% and the presence of eosinophilic microabscesses correlate with progressive or disseminated infection and thus are indicative of a poor prognosis.

Adolescent↗