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G Veress

Publications and source records attributed to G Veress.

At least 19 recordsLinked to original sources
PubMed2000

Additional human papillomavirus types detected by the hybrid capture tube test among samples from women with cytological and colposcopical atypia.

The type specificity of the human papillomavirus (HPV) Hybrid Capture Tube (HCT) test was evaluated by using typing with PCR (MY09-MY11)-restriction fragment length polymorphism (RFLP) and sequencing. All samples HCT test positive for only low-risk HPV (n = 15) or only high-risk HPV (n = 102) were confirmed, whereas 9 of 12 HCT test double-positive samples contained only high-risk HPV types as determined by PCR-RFLP. Several high-risk HPV types (HPV-53, -58, -62, -66, -CP8304, and -MM4) not included in the HCT test were indeed detected, indicating a broader detection range with retained distinction between low-risk and high-risk HPV types.

Cervix Uteri↗
PubMed1999

Functional significance of sequence variation in the E2 gene and the long control region of human papillomavirus type 16.

The long control region (LCR) and the E2 protein of human papillomaviruses (HPV) are the most important viral factors regulating transcription of the viral oncogenes E6 and E7. Sequence variation within these genomic regions may have an impact on the oncogenic potential of the virus. Sequence variation in the LCR and in the E2 gene of human papillomavirus type 16 (HPV-16) isolates originating from cervical cancer patients from East Hungary was studied. In 30 samples, sequencing and/or single-strand conformation polymorphism analysis revealed variants belonging to the European variant lineage of HPV-16. These variants differed from the reference European clone only slightly in their E2 and LCR sequences. Three samples represented variants belonging to the Asian-American group. These differed from the published reference sequence at several positions in the LCR and E2 regions. Compared to the reference clone, the LCR clones of the European isolates showed very similar transcriptional activities, while that of an Asian-American isolate had approximately 1.7-fold increased activity. Most of the increased activity of the Asian-American isolate could be ascribed to nucleotide changes found at the 3' end of the LCR (nt 7660-7890). The transcriptional transactivation potentials of the HPV-16 E2 isolates differed only slightly from each other, and the differences seemed to be independent of the taxonomic position of the isolates.

Base Sequence↗
PubMed1998

Consistent polymerase chain reaction-single-strand conformation polymorphism pattern of human herpesvirus-8 in the course of classical Kaposi's sarcoma assumes its clonal origin.

There is emerging evidence that Kaposi's sarcoma-associated herpesvirus (KSHV or HHV-8) has a central role in the pathogenesis of Kaposi's sarcoma (KS). The occurrence of HHV-8 in classical KS biopsies is reported irrespective of its clinical stage (patch, plaque, nodular). HHV-8 was detected in 25 of 28 formalin-fixed paraffin-embedded classical KS samples by nested polymerase chain reaction. In addition, in six patients multiple tumors were available (n = 21). Single-strand conformation polymorphism (SSCP) analysis of the amplicons showed uniform SSCP pattern of samples belonging to the same patient regardless of whether the KS was multiplex or developed again years after the first excision. Most of the SSCP patterns were confirmed by further sequence analysis. The presence of the same sequence variant of HHV-8 in various samples of the same patient supports the clonal origin of classical Kaposi's sarcoma.

Herpesvirus 8, Human↗
PubMed1997

[Follow-up of high risk, human papillomavirus (HPV)-positive patients with cancer of the uterine cervix].

The study population consisted of 30 cervical cancer patients stage I.a-II.b. (FIGO) stages operated on according to the Wertheim technique. A parallel histological evaluation and HPV status determination were carried out on biopsies from the primary tumors and on the regional lymph nodes. A general primer mediated polymerase chain reaction (PCR) was performed at first and the samples not amplified were examined by type-specific primers. All except one primary tumors contained DNA-sequences characteristic for high risk HPV-types. The lymph nodes of these HPV-positive patients proved to be also HPV-positive with a frequency of 25/30 (83%). The frequency of the HPV-positivity was higher (100%) in the group of patients with HPV-18 positive status, than in the HPV-16 positive group. Two thirds of the evaluated regional lymph nodes were HPV-positive in the HPV-16 group of patients. The same HPV-types were harboured by the primary tumors and by the regional lymph nodes both in the HPV-16 positive and HPV-18 positive groups of patients. In the HPV-16-positive group of patients metastatic lymph nodes occurred with a frequency of 3/16, while the frequency of HPV-16 positivity in the same nodes was 11/15. In the group of patients with HPV-18 positivity the difference was even greater, 1/12 v. 12/12. Early recurrences were detected in a relation of 3 to 1 in the group of patients with histologically tumor-free and metastatic-positive lymph node status. At the same time all of the lymph nodes in this group with early recurrency (4/4) contained DNA-sequences characteristic for the HPV-18 type. These findings raise the hypothesis that the HPV-specific nucleic acids detected in the lymph nodes can be taken as sensitive indicators of metastases. The follow-up results support these hypothesis as patients with HPV-18 positive lymph node status showed early recurrencies and short survival that is poor prognosis not corresponding to the early stage of cervical cancer with histologically negative lymph node status.

Adult↗
PubMed1996

Follow-up of human papillomavirus (HPV) DNA and local anti-HPV antibodies in cytologically normal pregnant women.

The high level of progesterone during pregnancy may enhance the transcription and replication of genital human papillomaviruses (HPV) through the glucocorticoid/progesterone response element found in the long control region of the viral genome. In this study, cytologically and colposcopically healthy pregnant women were subjected to a follow-up examination. Samples from the uterine cervix were collected during early pregnancy (n = 39), in the third trimester (n = 31), and a few weeks after birth (n = 30). The presence of HPV DNA was detected by polymerase chain reaction (PCR), while local secretory anti-viral IgA antibodies were demonstrated by enzyme-linked immunosorbent assay using synthetic peptide antigens. Follow-up examination by PCR revealed HPV DNA persistence in 5 women. In 5 other cases, HPV positivity changed from negative to positive during the follow-up. There was 1 case which changed from positive to negative and 1 in which the HPV type changed during the study. Altogether, 12 of 39 women (31%) were shown to harbor HPV DNA at some time during follow-up. HPV DNA positivity increased from 18% during early pregnancy to 27% after birth (difference not significant). On the other hand, there was a significant rise in the level of local antibodies against HPV antigens (E2, E7, and L2) between samples collected in early pregnancy and those collected after birth (P < 0.0001). This may indicate the reactivation of genital HPV infections during late pregnancy.

Adolescent↗
PubMed1995

Correlation of human papillomavirus 16 and 18 with prognostic factors in invasive cervical neoplasias.

Forty-seven patients with cervical carcinoma were examined in order to correlate human papillomavirus (HPV) types with prognostic factors in invasive cervical neoplasias. Age, clinical stage, histological type, and grade and parity were analysed with respect to HPV status as determined by a general primer mediated polymerase chain reaction (PCR) or a type specific PCR. All but one sample (98%) harboured HPV sequences: HPV 16 was found in 26 cases (55%), HPV 18 in 19 cases (40%), and HPV 31 in 1 case. The presence of HPV 18 DNA was significantly associated with cancers developed below 40 years of age (P = 0.029). HPV 18 detection was associated with poor differentiation malignancy (P = 0.045) and histological types of poor prognosis (adenocarcinoma or nondifferentiated carcinoma; P = 0.006). HPV 18 positivity was also correlated with advanced clinical stages (FIGO II and III; P = 0.032). Parity and HPV status proved to be independent of each other (P approximately 0.99). Eighty-seven percent (27/31) of pelvic lymph nodes from HPV positive patients contained HPV DNA. The virus types found in lymph nodes were identical with those of the primary tumours in all cases. Virological results were compared to those obtained by routine histological examination. Only 6 of 27 patients with HPV positive lymph nodes had any histological evidence of metastasis. Nevertheless, the lack of metastasis as detected by histology does not exclude the possibility of relapses. Follow-up of the clinical prognostic significance of PCR detection of HPV in the possible sites of early metastases.

Adenocarcinoma↗
PubMed1994

[Blocked atrial extrasystole simulating impaired anterograde conduction during atrial stimulation].

In a 72-years-old patient with intermittent bradycardia definitive atrial pacing was considered. Surface ECG was misleading because of demonstrating 2:1 anterograde block during atrial stimulation at a rate of 100 beats per minute. Intracardiac recordings disclosed the bigeminal occurrence of premature atrial contractions. Every second stimuli fall within the premature atrial contraction's refractory period simulating impaired AV-conduction. Our report emphasizes the importance of intracardiac recordings during atrial stimulation in order to evaluate the anterograde conduction before pacemaker implantation.

Aged↗
PubMed1994

[AV reciprocating tachycardia with intermittent bundle branch block in latent Wolff-Parkinson-White syndrome].

This report deals with a patient with concealed Wolff-Parkinson-White syndrome in whom both narrow and wide QRS tachycardias were observed. The simultaneous occurrence of various QRS morphology during supraventricular tachycardia results in a challenging diagnostic ECG problem. The cycle length during tachycardia with left bundle branch block was longer than the cycle length during narrow QRS supraventricular tachycardia and with functional right bundle-branch block. Electrophysiologic studies revealed fixed V-A conduction time and increased V-A conduction during tachycardia with left bundle branch block. These studies suggested the presence of a concealed left-sided anomalous pathway. Differentiation between intra- and extranodal re-entry and therapeutic modalities are also discussed.

Adrenergic beta-Antagonists↗
PubMed1994

Human papillomavirus DNA and anti-HPV secretory IgA antibodies in cytologically normal cervical specimens.

Cervical specimens collected from 163 cytologically healthy women were screened for the presence of human papillomavirus (HPV) DNA and anti-HPV secretory IgA antibodies. HPV DNA was detected by a general primer mediated polymerase chain reaction (PCR), which amplifies a conserved region from the L1 ORF of genital HPVs. The PCR products were typed by restriction enzyme digestion. A total of 35 samples (21.5%) were positive for HPV DNA (13 samples for HPV 6, 6 for HPV 16, 3 for HPV 18, and 13 for untypeable HPV X). HPV DNA positivity was significantly higher among women under 25 years of age (34.8%) than among the older patients (12.4%) (P < 0.001). An enzyme-linked immunosorbent assay (ELISA) using synthetic peptide antigens was carried out to detect local secretory IgA antibodies against the following HPV specific antigens: HPV 16 E2, HPV 16 E7, HPV 16 L1, HPV 16 L2, and HPV 11 L2. Thirty-four secretions (20.9%) were found to react with at least one of the oligopeptides. Anti-HPV IgA positivity was the highest among women aged 25-32 years, and it was significantly lower in both the younger and the older age groups (P < 0.05). Correlation between HPV DNA and anti-HPV IgA detection was rather weak: anti-peptide IgA positivity was 34.3% (12 of 35) among HPV DNA positive patients compared to 17.2% (22 of 128) among HPV DNA negative women (P < 0.05). The fluctuating course of latent HPV infections should be considered in evaluating the low level of correlation between HPV DNA and anti-HPV IgA positivity.

Adolescent↗
PubMed1994

Analysis of re-entry mechanism in a patient with concealed Wolff-Parkinson-White syndrome.

This report deals with a patient with concealed Wolff-Parkinson-White syndrome in whom both narrow and wide QRS tachycardias were observed. The simultaneous occurrence of various QRS morphology during supraventricular tachycardia results in a challenging diagnostic ECG problem. The cycle length during tachycardia with left bundle branch block was longer than the cycle length during narrow QRS supraventricular tachycardia and with functional right bundle-branch block. Electrophysiologic studies revealed an increased V-A conduction time during tachycardia with left bundle branch block. These studies suggested the presence of a concealed left-sided anomalous pathway. Differentiation between intra- and extranodal re-entry and therapeutic modalities are also discussed.

Adult↗
PubMed1993

Infra-His blocked premature atrial contractions simulating 2:1 sinoatrial block in a patient with an atrio-His bypass tract.

Blocked atrial premature contractions simulated 2:1 sinoatrial block because those were superimposed on the T wave of the preceding sinus beats and were not visible on the surface electrocardiogram of our patient. Additionally, His bundle recordings and premature atrial stimulation demonstrated the presence of an atrio-His anomalous pathway. The premature atrial contractions traveled anterograde via the anomalous pathway and were blocked distally to the His bundle. The term, pseudosinoatrial block, is used to describe the arrhythmia because there was no evidence of an intrinsic abnormality of sinus node function and sinoatrial conduction.

Bradycardia↗
PubMed1993

Genital human papillomavirus (HPV) infection in Hungarian women.

The prevalence of genital human papillomavirus (HPV) infection in Hungarian female populations is not essentially different from that found in other countries of Europe and North-America. Using filter in situ hybridization (FISH), we found that, in a group of cytologically normal women some low risk HPV types (such as HPV 6 and 11) and the most important high risk HPV types (HPV 16 and 18) were present in 23% and 8%, respectively. Eighty-eight percent of condyloma acuminatum patients harboured HPV 6 or HPV 11 in their tumours. On the other hand, in precancerous lesions (cervical intraepithelial neoplasia, CIN) HPV 16 was the predominant type, being present in 29-48% of patients, depending on the detection method used (Southern blot hybridization vs. polymerase chain reaction). The detection rate of high risk HPV types was found to rise with the increasing severity of cervical neoplasia. Finally, 48% of invasive cervical carcinoma specimens were positive for HPV 16 DNA in a type-specific polymerase chain reaction. For patients with HPV 16 positive primary tumours, all but one lymph node metastases and about 30% of histologically normal lymph nodes proved positive for HPV 16 DNA. Our results--in accordance with the numerous data found in literature--seem to confirm the hypothesis that certain HPV types are greatly involved in the development of cervical cancer.

Carcinoma↗
PubMed1992

Oral contraceptive use and human papillomavirus infection in women without abnormal cytological results.

Both experimental and epidemiological data support the idea that oral contraceptive (OC) use may have a stimulating effect to a certain point on cervical carcinogenesis. The current investigation tries to answer the question whether OC use might have an influence on early human papillomavirus (HPV) infections. A total of 425 women without abnormal cytological results were examined colposcopically, and filter in situ hybridisation (FISH) was used to determine the presence of human papillomavirus (HPV) types 6, 11, 16 and 18. Eighty-one cervical specimens (19.1%) were found to be positive for one or more of the HPV types in FISH. HPV positivity was found to correlate with age and parity, being the highest among women under 25 and with less than two births. The use of OCs was inversely correlated with the presence of ectopy or dysplasia in this group of women. On the other hand, HPV positivity was not significantly higher among OC users than among non-users in any colposcopic group. Neither the type of pill used, nor the duration of use had any significant effect on HPV positivity. Further investigations are needed to evaluate the effects of OC use on more severe HPV-induced cervical lesions.

Age Factors↗
PubMed1992

Amplification of human papillomavirus type 16 transforming genes from cervical cancer biopsies and lymph nodes of Hungarian patients.

We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical surgery on 35 women were also analyzed. Lymph nodes originating from 19 patients whose cervical biopsy specimens were negative for HPV 16 seemed to lack HPV 16 sequences. For 16 women with positive PCR results for cervical biopsy specimens, 9 of 10 lymph node metastases were positive in the PCR, while 11 of their 36 histologically negative lymph nodes were also shown to contain HPV 16 DNA.

Adult↗