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Biomedical subjects

G V Hillyer

Publications and source records attributed to G V Hillyer.

At least 127 records · Page 7Linked to original sources
PubMed1979

Solubilization of antigens of Fasciola hepatica which react with antibodies to Schistosoma mansoni.

Extracts of Fasciola hepatica adult worms contain antigens reactive with antisera prepared against Schistosoma mansoni adult worms. These antigens are poorly solubilized when homogenized in a phosphate buffered saline (PBS) solution and pellet readily when subjected to high speed centrifugation. Solubilization is improved greatly by the addition of sodium dodecyl sulfate (0.03%) to the PBS. When this is done, one obtains approximately the same total amount of crude Lowry reactive material as with PBS extraction followed by high speed centrifugation but antigenic reactivity to an anti-S. mansoni antiserum increases enormously. The antigens liberated from F. hepatica SDS extraction are largely materials under 200,000 MW and over 60,000 MW.

Animals↗
PubMed1978

Laurell crossed immunoelectrophoresis and affinity chromatography for the purification of a parasite antigen.

Laurell crossed immunoelectrophoresis (two-dimensional electroimmunodiffusion) was used to prepare minute amounts of purified parasite antigens complexed with their precipitating antibodies obtained from rabbits. These complexes, emuslified in Freund's complete adjuvant, were then used to prime rabbits for selective production of precipitins to the complexed antigens when the animals were later boosted with whole parasite extract. The IgG antibody from the monospecific antiserum recovered was then utilized in affinity chromatography to isolate from the crude parasite antigen large amounts of specific antigen in one step. Thus the combination of preparatory crossed immunoelectrophoresis for immunization using complexed antigen and affinity chromatography with monospecific antibody offers a powerful procedure for the rapid isolation of specific antigens.

Animals↗
PubMed1978

Demonstration of a common antigen between Schistosoma mansoni and Fasciola hepatica.

The presence of a common antigen between Schistosoma mansoni eggs and Fasciola hepatica adult worms was demonstrated by utilizing in an anti-S. mansoni adult worm antiserum. Although not one of the three major serologic S. mansoni egg antigens, its complete cross-reactivity suggests that serologic tests done with these crude antigenic extracts will result in many false-positive cases in areas where both parasites are endemic.

Antigens↗
PubMed1977

Partial purification of fasciola hepatica antigen for the immunodiagnosis of fascioliasis in rats.

When F. hepatica adult worm antigen is fractionated with Sephadex G-200, four major peaks are obtained. The first peak, when used as antigen in counterelectrophoresis, reacts with the serum of normal rats as well as from rats infected with F. hepatica for 5 weeks or longer. The crest and descending portion of peak II and the third peak are nonreactive with normal rat serum, but highly reactive with the serum of rats with F. hepatica. In addition, F. hepatica antigens cross-reacting with S. mansoni adult worm antisera are absent in these fractions. It is suggested, then, that these fractions of F. hepatica adult worm antigen be utilized for the immunodiagnosis of fascioliasis by CEP. Optimum antigen concentration for this test has been determined to be approximately 1.80 mg/ml protein.

Animals↗
PubMed1976

Use of immunologic techniques to detect chemotherapeutic success in infections with Fasciola hepatica. I. Rabbit infections.

Rabbits infected with Fasciola hepatica develop precipitins to adult worm heomogenates, as observed by Ouchterlony double immunodiffusion (ID) and counterelectrophoresis (CEP). When they are successfully treated with a fasciolicidal drug such as rafoxanide at 5, 6, or 11 weeks of infection their precipitins drop dramatically by 2 weeks post-treatment, they are virtually negative by 4 weeks, and have no detectable precipitins by 5 or 6 weeks post-treatment. The results suggest that ID or CEP can be utilized to show chemotherapeutic success in rabbits infected with F. hepatica and warrant further studies as to their possible application in human fascioliasis.

Animals↗
PubMed1976

Schistosome antigens in the circulation of chimpanzees infected with Schistosoma japonicum.

Chimpanzees infected with Schistosoma japonicum develop circulating schistosome antigens in their circulation between 6 and 9 weeks post-exposure. The minimum number of circulating antigens ranges from one to three. These antigens also cross-react with an antiserum against S. mansoni adult worms. Clearance of these antigens from the circulation several weeks later typically occurs. Persistence of these antigens could result in the observed renal damage.

Animals↗