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Biomedical subjects

G Tringali

Publications and source records attributed to G Tringali.

At least 55 records · Page 3Linked to original sources
PubMed1986

Isolation on "VERO" cells of a spotted fever group rickettsia "Mortilli strain" from Rhipicephalus sanguineus.

The isolation of a Spotted Fever Group (SFG) Rickettsia "Mortilli strain" from brown dog ticks Rhipicephalus sanguineus removed from dogs associated with a confirmed case of Boutonneuse Fever (BF) in a site of a Western Sicily is reported. The need is stressed for isolation and adaption on cell cultures of SFG rickettsial strains for analysis of antigenic structure employing modern methodology.

Animals↗
PubMed1986

Epidemiology of boutonneuse fever in western Sicily. Distribution and prevalence of spotted fever group rickettsial infection in dog ticks (Rhipicephalus sanguineus).

The distribution and prevalence of spotted fever group rickettsial infection in the ixodid dog tick Rhipicephalus sanguineus were found to occur at a rate of 19.7% with variation related to geographic and sociooccupational factors. A higher rate of infection was demonstrated in ticks removed from dogs associated with documented cases of boutonneuse fever. The results fit into available clinical and seroepidemiologic data on Rickettsia conorii infection in western Sicily.

Animals↗
PubMed1986

Frequent occurrence of hepatic lesions in boutonneuse fever.

Seven consecutive Sicilian patients with boutonneuse fever but without clinical symptoms of hepatic disease underwent hepatic biopsy and had similar hepatic lesions. Foci of hepatocellular necrosis were infiltrated with predominantly mononuclear leukocytes. No intact Rickettsia conorii were identified in the tissues by immunofluorescence. The apparent high frequency of viscerotropism in boutonneuse fever conforms to the recent observations of severe illness in what had often been described previously as a benign rickettsiosis.

Adult↗
PubMed1986

Atypical behaviour of specific antitreponemal IgM in active and treated syphilis.

Negative 19S (IgM) test in patients with active untreated syphilis and reactive 19S (IgM) test in a group of patients with treated syphilis are reported. The practical value of IgM test in routine serodiagnosis and monitoring of the treatment of syphilitic infection as well as the theoretical aspects of the IgM reactivity are discussed.

Adult↗
PubMed1985

Circulating immune complexes in Fièvre boutonneuse.

Circulating immune complexes (CIC) occurred in 36% of a group of 25 patients with Fièvre boutonneuse. CIC were present only in the first week of the disease and there was no evidence of other humoral immunological abnormalities or alterations of the coagulation factors studied. The presence of CIC was not associated with a more severe clinical manifestation or with symptoms or tissue injuries. It is considered that CIC do not play a major role in Fièvre boutonneuse.

Antibodies, Bacterial↗
PubMed1984

A sero-epidemiological survey of asymptomatic cases of Boutonneuse fever in western Sicily.

963 sera from contacts, persons from various localities, and blood donors were examined with a commercially produced kit for micro-immunofluorescence for the presence of antibodies to Rickettsia conorii. 10.6% of sera were serologically positive. The higher rates of positivity were observed in sera of contacts (19%) and persons from Mussomeli (20%) and Ustica (18.4%), the lower rates in blood donors from Palermo (3.5%). These results support the view that there is an occupational risk factor related to a rural environment.

Antibodies, Bacterial↗
PubMed1984

Demonstration of spotted fever group rickettsiae in the tache noire of a healthy person in Sicily.

A human case of rickettsial infection occurred in Sicily following tick bite. The patient did not have fever, the typical nodular rash, or other symptoms of illness other than development of a tache noire containing spotted fever group rickettsiae, which were demonstrated by immunofluorescence. A high titer of antibodies of the IgG class suggests that the patient may have had previous exposure to Rickettsia conorii or a related spotted fever group rickettsia. An anamnestic response may be hypothesized to have conferred partial immunity, with resulting containment of rickettsiae at the site of inoculation.

Antibodies, Bacterial↗
PubMed1983

[Seroimmunologic studies in boutonneuse fever. I. Evaluation of a commercial micro-immunofluorescence kit in the serodiagnosis of boutonneuse fever].

The diagnosis of Boutonneuse Fever usually depends on clinical evidence (summer occurrence, fever, tache noire at the site of the tick bite, in 30-70% of cases, erythemato -papular rash, prompt response to chloramphenicol or tetracycline treatment). Serological confirmation is difficult since the only diagnostic procedure currently feasible, the Weil-Felix test, is not specific. Other more specific diagnostic procedures (agglutination, complement fixation, ELISA, indirect immunofluorescence tests) are beyond the possibilities of most laboratories (antigens are not available from the market). In the present paper, results obtained with a new commercially produced kit for indirect immunofluorescence are reported. Sera from patients with infectious and non infectious diseases as well as Boutonneuse Fever (at various stages of illness, from 6 days to 12 months) were examined. Sera from blood donors were also included. Specificity and sensitivity were satisfactory as well as reproducibility of results. Some apparently false positivities must be related to the present epidemiological pattern in western Sicily, and namely to the incidence of asymptomatic cases of Boutonneuse Fever, as demonstrated by recent works.

Antibodies, Bacterial↗
PubMed1977

[Electroimmunodiffusion reaction in the diagnosis of syphilis (author's transl)].

A group of 800 human sera, obtained from syphilitic and nonsyphilitic individuals were tested in the counterimmunoelectrophoresis (CIE) technique with Reiter protein antigen. The sera were assayed for comparison in the following treponemal and nontreponemal tests: standard serologic tests for syphilis with cardiolipin; complement fixation (Kolmer 1/5 vol) with Reiter protein antigen (RPCF); T. pallidium immobilization (TPI) test; haemoagglutination test for T. pallidum antibodies (TPHA). The sensitivity and specificity of CIE test, its simplicity, the low cost, the possibility of practical application as a routine test for syphilis serology were discussed.

Antibodies↗