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Biomedical subjects

G Tricot

Publications and source records attributed to G Tricot.

At least 181 records · Page 10Linked to original sources
PubMed1985

Lymphoid interstitial pneumonitis in acquired immunodeficiency syndrome-related complex.

Three patients had a typical clinical, functional, and histologic pattern of lymphoid interstitial pneumonitis (LIP). All 3 also had a marked follicular and interfollicular hyperplasia of peripheral lymph nodes. A benign lymphocytic infiltration was seen in the kidneys in 2 patients, in the liver of 1 of these, and in the nasopharynx and the bone marrow in another. This LIP, associated with other diffuse benign lymphocytic infiltrates, could be part of AIDS-related manifestations. The patients originated from central Africa (2 patients) and Haiti (1 patient) where AIDS is endemic. They had several immune abnormalities frequently observed in AIDS such as a polyclonal hyperimmunoglobulinemia and, in the 2 patients studied, a decreased number of T4 lymphocytes and an inverted T4/T8 blood lymphocyte ratio. All 3 patients had serum antibodies to human T-cell leukemia viruses.

Acquired Immunodeficiency Syndrome↗
PubMed1985

A case of histiocytosis X-central type. Histological, immunohistochemical and electronmicroscopical analysis.

We report a case of Histiocytosis X (HX) associated with a high bone marrow and peripheral blood monocytosis in a 70 yr-old man. The histiocytic clusters in the skin showed the characteristics of epidermal Langerhans cells (LC): positivity for OKT6, OKIa1, ATP-ase and presence of Birbeck granules. Histiocytes in all other locations showed positivity for OKM1, OKIa1, acid P-ase and absence of Birbeck granules. The circulating monocytes were also negative for OKT6 and ATP-ase and lacked Birbeck granules. From these data we assume that the proliferation of monocytes in the bone marrow was the primary event, resulting in the release of large numbers of circulating monocytes and histiocytic proliferation in various tissues. These histiocytes acquired differing characteristics according to the microenvironment in which they underwent their final maturation. In the skin they differentiated into cells presenting the morphological, enzyme-and immunohistochemical characteristics of LC.

Aged↗
PubMed1984

8;21 Translocation in acute myeloid leukemia. An ultrastructural study.

An ultrastructural study was performed in four patients with acute myeloid leukemia (AML) and 8;21 translocation. The most prominent pathologic features of the leukemic cells were: (1) a high frequency of nuclear blebs, which have been associated with an aneuploid karyotype; (2) nucleocytoplasmic asynchrony in early myeloid precursors, mature polymorphonuclear leucocytes (PMN) and in megakaryocytes; (3) abnormal granule formation with the presence of Auer rods, giant granules (pseudoChediak-Higashy) containing small vesicles, primary granules, and rod-like structures; absence of secondary granules in the more mature myeloid cells and the presence of hypogranular forms; (4) the presence of "labyrinths" consisting of myeloperoxidase (MPO) negative circular tubuli, interwoven with MPO positive endoplasmic reticulum; (5) the lack of hiatus leukemicus. Although none of these features separately is pathognomonic for 8;21 AML, we can conclude that the combination of these ultrastructural findings characterizes the leukemic myeloid cells of patients with 8;21 translocation in AML.

Adult↗
PubMed1984

A reliable method with good cell preservation for the demonstration of peroxidase activity in human platelets and megakaryocytes.

We have tried to improve existing methods for demonstration of platelet peroxidase (PPO) in human platelets and megakaryocytes by introducing a fixation of 0.1% glutaraldehyde prior to incubation in the DAB medium. This prefixation with low concentration of glutaraldehyde preserves excellent morphological detail and does not inhibit PPO activity. All 23 platelet-rich plasma samples show PPO reaction product in the dense tubular system after incubation in DAB medium with 0.003% H2O2. When 0.01% H2O2 is used in excessive DAB medium, PPO activity can also be demonstrated in platelets and megakaryocytes of bone-marrow cell suspensions. This method can be used for the identification of megakaryoblasts in acute non-lymphocytic leukemia, myelodysplastic syndromes and in blastic crisis of chronic myeloid leukemia. PPO cytochemistry can be combined with postfixation in a OsO4-ruthenium red mixture. This method reveals alpha-granules, dense bodies, microtubuli, glycogen, mitochondria, dense tubular system and invaginated membrane system in the same platelet and is useful for investigation of platelet ultrastructure.

Blood Platelets↗
PubMed1984

Bone marrow transplantation performed as first-time treatment in two cases of secondary acute myeloid leukemia.

Two young patients with secondary acute myeloid leukemia were treated with allogeneic bone marrow transplantation as first-line treatment for their disease. High dose cytosine arabinoside was added to the conventional preparative regiment of cyclophosphamide and total body irradiation. No major problems were encountered to the immediate post-transplantation period. Complete remission of the acute myeloid leukemia was noted in both patients, with no evidence of recurrent disease at 15+ and 13 months. One patient developed severe pancytopenia 10 months after grafting with the presence of antibodies against platelets and granulocytes from the bone marrow donor. She died 3 months later from a generalised Aspergillus septicemia, probably precipitated by therapy with high doses of methylprednisolone. The other patient is alive in excellent clinical condition and in hematologic remission. Bone marrow transplantation must be taken into consideration as first-line therapy in any young patient who is suffering from a refractory type of leukemia and who has a suitable donor.

Benzene↗
PubMed1984

Interstitial 9q- deletions in hematologic malignancies.

Sixteen patients with interstitial deletions of the long arm of chromosome #9 (9q-). were studied. From our observations and the findings of ten other cases in the literature, it can be deduced that the anomaly is almost exclusively found in myeloproliferative disorders and that it rarely occurs as the sole anomaly; however, in more than one-third of the cases, it was associated with a t(8;21) and occurred as a secondary event. The deletion appears to be interstitial, the breakpoints are somewhat variable, and the region carrying the abl oncogene was never involved.

Acute Disease↗
PubMed1984

Distribution of lymphocyte subsets in a case of angiofollicular lymph node hyperplasia.

A series of monoclonal and polyclonal antibodies was used to determine the distribution of lymphocyte subsets in frozen tissue sections of a case of angiofollicular lymph node hyperplasia (ALNH), intermediate type. Primary follicles and mantle zones of secondary follicles consisted of BA1+OKIa1+-C3RTo5+sIgM+sIgD+ lymphocytes. Poorly developed follicular centers reacted with OKIa1, C3RTo5, DRC-1 and anti-IgM but contained no OKT4+Leu-3a+ or Leu-7+ cells. Mantle zones and primary follicles contained more OKT4+Leu-3a+ and less OKT8+ cells than normally observed. The interfollicular area consisted mainly of OKT4+Leu-3a+OKIa1- helper/inducer T-cells, admixed with some OKT8+ suppressor/cytotoxic T-cells and OKIa1+ interdigitating reticulum cells. Polyclonal plasma cells were found both in follicular centers and interfollicular areas. Based on the analogy to the peripheral lymphoid tissue in nude mice and in children with variable thymic dysfunction, it is suggested that the abnormal distribution of immunoregulatory T-cells may be of pathogenetic significance and may account for the morphology of the lymphoid follicles in ALNH.

Adolescent↗
PubMed1984

Bone marrow histology in myelodysplastic syndromes. I. Histological findings in myelodysplastic syndromes and comparison with bone marrow smears.

Forty bone-marrow trephine biopsies of patients with myelodysplastic syndromes (MDS) were studied and compared with bone marrow smears. It proved possible to make the diagnosis of MDS on the basis of bone-marrow biopsies. Features of abnormal erythropoiesis and myelopoiesis, although different from those observed in smears, were easily detectable. Moreover, obvious advantages of bone-marrow biopsy over bone-marrow smears were found. Firstly, the biopsy in MDS gives an exact assessment of the cellularity; secondly, the presence of reticulin fibres, increased in the majority of the cases, can be evaluated; thirdly, dysmegakaryopoiesis is more easily detected in trephine biopsies than in smears; finally, abnormal behaviour of myeloblasts, clustering centrally in the bone marrow, is frequently observed before the smears show an excess of myeloblasts. The bone-marrow biopsy also has a role in difficult cases, where the diagnosis of MDS can not be established on just bone-marrow smears. The five types of MDS, as described by the FAB group, do not represent recognizable histological entities.

Adult↗
PubMed1984

Bone marrow histology in myelodysplastic syndromes. II. Prognostic value of abnormal localization of immature precursors in MDS.

A study has been performed of 40 bone marrow trephine biopsies from patients with myelodysplastic syndromes (MDS). Histology, more specifically the abnormal localization of immature precursors (ALIP), cytology and karyotype analysis were correlated with survival time and the prognostic value of these factors was determined. The presence or absence of ALIP proved to be highly significant in predicting the duration of survival (P = 0.007): the presence of ALIP predisposes patients to early death with high probability of developing acute myeloid leukaemia (AML), whereas the absence of ALIP carries a better prognosis with more long-term survivors and low probability of transformation to AML. The presence or absence of ALIP was not correlated with increased mortality from infection and/or bleeding. In contrast with ALIP, the main histological parameter, we were not able to obtain statistically significant prognostic information for cytology (FAB classification) or karyotype analysis (normal or abnormal chromosomes). We conclude that bone marrow biopsy provides useful information for the prognosis of MDS and that the presence of ALIP has greater predictive value than the more commonly used parameters deduced from cytology and karyotype.

Adult↗
PubMed1984

Low dose cytosine arabinoside (Ara C) in myelodysplastic syndromes.

Myelodysplastic syndromes (MDS) are acquired bone-marrow disorders, characterized by a decreased ability of the haemopoietic cell to differentiate, resulting in peripheral cytopenias. The majority of patients will die either from acute myeloid leukaemia or from infection and/or haemorrhage. Thirty-eight courses of low dose Ara C were administered in 26 MDS patients. Nineteen courses (50%) were associated with good (12) or partial (7) response. Three complete remissions were observed. The median duration of response overall was 19.5 weeks, 26 weeks for the good and 10 weeks for the partial responders. A high incidence of treatment failure was seen in patients treated after transition to AML. Major complications were observed during 14 courses and mortality was directly related to therapy in five patients. Platelet transfusions were required during 26 courses. Aggravation of peripheral-blood cytopenia during the first weeks and hypocellularity of bone-marrow aspirates at the end of therapy suggest that low-dose Ara C exerts its main activity by suppression of leukaemic growth, rather than by induction of differentiation in malignant cells. Our results in MDS patients demonstrate that low-dose Ara C can be of value in severe cytopenia and can decrease the proportion of leukaemic cells in the bone marrow, but the danger of treatment should not be underestimated.

Adult↗
PubMed1984

[Therapeutic indications in digestive localizations of non-Hodgkin's lymphoma in adults].

One third of all extranodal sites of non-Hodgkin's lymphomas are localized to the digestive tract. Forty-six cases with digestive tract localization were studied, of which 15 were localized forms. Histologically, 70 p. cent were diffuse lymphomas with poor prognosis. The present survival rate for localized forms following chemotherapy is 77 p. cent, which plateaux at two years, as compared to 34 p. cent for disseminated forms. Treatment of digestive tract localizations should be similar to that of nodal forms with the same histology. With the development of intensive chemotherapeutic regimens over the last few years, the cure rate is hoped to attain 65 p. cent in disseminated forms, and 87 p. cent in localized forms.

Adolescent↗
PubMed1983

Hypercalcemia and diffuse osteolytic lesions in the acute phase of chronic myelogenous leukemia. A possible relation between lymphoid transformation and hypercalcemia.

A patient with blastic crisis of chronic myelogenous leukemia (CML) is presented. The acute phase was localized in the lymph nodes and bones, causing severe osteolytic lesions and hypercalcemia. The blast cells were undifferentiated in light microscopy and by histochemical stains. As viewed under electron microscopy, a considerable proportion of the blast cells were of myeloid origin, while immunologic markers and cytogenetics indicated a lymphoid origin. It seems plausible that the patient had a mixed myeloid-lymphoid blast crisis, but that the lymphoid blasts were responsible for the severe osteolytic lesions and the hypercalcemia.

Acute Disease↗