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Biomedical subjects

G Trautwein

Publications and source records attributed to G Trautwein.

At least 55 records · Page 3Linked to original sources
PubMed1990

Detection of canine distemper viral antigen in formalin-fixed and paraffin-embedded tissue of a fitch (Mustela putorius), using an immunoperoxidase technique.

The present study describes how a naturally infected fitch (Mustela putorius) caused an outbreak of canine distemper in a colony of insufficiently vaccinated dogs. The detection of canine distemper virus (CDV) on paraffin sections of different organs of the fitch and one of the dead dogs was achieved using a monoclonal antibody against the nucleocapsid protein (NP) of CDV and the avidin-biotin-peroxidase complex (ABC) technique.

Animals↗
PubMed1990

Detection of circulating immune complexes in MRL mice with different forms of glomerulonephritis.

Serum levels of circulating immune complexes (CIC) in MRL mice were determined using the 125I-C1q binding test and a conglutinin enzyme assay. The serum 125I-C1q and conglutinin binding capacity were correlated to the development of different forms of glomerulonephritis (GN) detected by light microscopy. The study was carried out with a total of 132 MRL/MpJ-lpr/lpr and MRL/MpJ(-)+/+ mice of different age groups. From the age of 3 months on an enormous elevation of C1q- and conglutinin-binding CIC occurred in MRL/MpJ-lpr/lpr mice, while in MRL/MpJ(-)+/+ mice the mean values of CIC remained low in all age groups studied. The results corresponded to histopathological glomerular findings. In lpr/lpr-mice, from the age of 3 months on, extremely severe proliferative forms of GN occurred. In most cases of +/+(-)mice examined comparatively mild glomerular lesions were found. In serum samples of Han:NMRI and C3H/He mice an increase of conglutinin-binding material was found in certain age groups, while the levels of 125I-C1q binding activity remained low in mice of all age groups studied.

Age Factors↗
PubMed1989

Immunoglobulin lambda-light-chain-derived amyloidosis (A lambda) in two horses.

Tumorous amyloid deposits in the nasal mucosa of two horses differed from generalized AA-amyloidosis with respect to clinical features, organ distribution, and resistance to KMnO4 treatment. Using a panel of antibodies directed against different human amyloid fibril proteins and employing the peroxidase-anti-peroxidase (PAP) technique, we showed the described equine amyloid to be A lambda-type, as demonstrated by immunohistochemical cross-reactivity. Consequently, we identified a second amyloid class in horses and showed that immunoglobulin light-chain-derived amyloid may also be present in animals.

Amyloid↗
PubMed1989

Viral antigen distribution in the central nervous system of cattle persistently infected with bovine viral diarrhea virus.

Distribution of viral antigens in the central nervous system of 25 cattle with a persistent bovine viral diarrhea virus (BVDV) infection was studied. Using a polyclonal antiserum produced in pigs and the direct immunofluorescence and immunoperoxidase technique, BVDV antigen was located exclusively in neurons. Predilection sites for viral persistence were cerebral cortex and hippocampus; in other areas of brain and spinal cord, viral antigens were in single neurons or small groups of neurons. There was no morphological evidence of cellular alteration due to viral persistence. Perivascular lymphocytic infiltrations were in affected nervous tissue. It is concluded that the central nervous system is an important location for persistence of BVDV.

Animals↗
PubMed1989

[The pathogenesis of glomerulonephritis in MRL mice--murine lupus erythematosus].

In a sequential study the spontaneously occurring glomerular lesions in MRL mice were analysed by light and immunofluorescence microscopy. The study was carried out with a total of 265 MRL mice of both substrains (MRL/Mp-lpr/lpr and -+/+) and of different age-groups. The glomerular lesions were classified in five forms of glomerulonephritis (GN): focal GN, diffuse GN, intra- and extracapillary-proliferative (crescentic) GN, membranoproliferative GN, and membranous GN. Crescentic GN was exclusively seen in mice of substrain lpr/lpr. Marked substrain-related differences concerning frequency, age of onset and severity of glomerular lesions were found. The occurrence of severe glomerulopathies in mice of substrain lpr/lpr at the age of 3 months was associated with a highly significant increase of circulating immune complexes.

Animals↗
PubMed1987

Sequential study of vasculitis in MRL mice.

The frequency, age of onset and organ distribution of spontaneously occurring vasculitis was examined in a sequential study with 170 MRL mice of both substrains. Necrotizing vasculitis was seen in 55.8% of MRL/Mp-lpr/lpr mice studied, beginning at the age of 3 months. The kidney and urinary bladder were most frequently involved. In MRL/Mp- +/+ mice necrotizing vasculitis was much less frequently present (7.6%), beginning at the age of 18 months, and was seen only in the kidney, stomach and testes. In both substrains mononuclear infiltration of pulmonary vessel walls preceded the occurrence of necrotizing arteritis in other organs. The immunofluorescence study revealed the presence of immune complex components (immunoglobulin G, C3, murine leukaemia virus antigen gp71) in the vessel walls of the renal arteries of six out of 36 lpr/lpr mice with necrotizing arteritis.

Animals↗
PubMed1986

The role of cartilage canals in the pathogenesis of experimentally induced polyarthritis.

Porcine articular cartilage from cases of experimentally induced Erysipelas polyarthritis, a comparative model of rheumatoid arthritis (RA) in man, was examined with different histological and immunohistochemical techniques. The preexisting canals in articular cartilage played a crucial role during the flooding and deposition of arthritogenic microorganisms deep into the cartilage matrix. Subsequently this vascularized tissue mediated the same inflammatory reactions in hyaline cartilage of young animals as seen in other connective tissues. However, these stereotypical responses to injury were modulated by the unique composition and structure of articular cartilage.

Animals↗