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Biomedical subjects

G Thomas

Publications and source records attributed to G Thomas.

At least 883 records · Page 49Linked to original sources
PubMed1970

An adhesive surface sampling technique for airborne viruses.

A mixture of sucrose, glycerol and bovine serum albumin produces a stable coating in a Petri dish which remains adhesive for up to an hour when exposed in a slit sampler. Virus aerosols can be collected on this surface followed by the direct addition of cell cultures to demonstrate the presence of viable virus. The technique is applicable to the Andersen sampler. A modified version of this sampler has been produced with the same particle collection efficiency as the standard Andersen sampler. The plaque counts obtained by the adhesive surface sampling technique are believed to give an indication of the number of particles collected bearing viable virus.

Air Microbiology↗
PubMed1970

Samplig rabbit pox aerosols of natural origin.

Natural aerosols of rabbit pox virus produced by groups of infected rabbits were sampled with a slit and Andersen sampler using an adhesive surface sampling technique (Thomas, 1970). The higher rate of successful recoveries of airborne virus compared with a previous report is believed to be due to the much larger volume of air sampled by this technique and to the processing of the whole of the sample collected by the direct addition of a cell suspension to demonstrate the presence of viable virus.

Air Microbiology↗
PubMed1969

Application of electron diffraction to biological electron microscopy.

Three methods by which electron diffraction may be applied to problems in electron microscopy are discussed from a fundamental point of view, and experimental applications with biological specimens are demonstrated for each case. It is shown that wide-angle electron diffraction provides valuable information for evaluating specimen damage that can occur either during specimen preparation or while in the electron beam. Dark-field electron microscopy can be used both to enhance the image contrast and to provide highly restricted and therefore highly specific information about the object. Low-angle electron diffraction provides quantitative information about the object structure in the range from 20 A to approximately 1000 A. Lowangle electron diffraction also demonstrates the important role of Fourier contrast with biological specimens, which are usually characterized by structural features with dimensions of 20 A or larger.

Electrons↗