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Biomedical subjects

G Theodoropoulos

Publications and source records attributed to G Theodoropoulos.

At least 55 records · Page 3Linked to original sources
PubMed1983

HBeAg/anti-HBe antigenic system in liver diseases in Greece.

HBeAg and anti-HBe were sought by RIA in the serum of 320 HBsAg-positive and 27 HBsAg-negative Greek patients with acute and chronic liver diseases. The incidence of HBeAg and anti-HBe in the group of 60 patients with acute hepatitis B was 23% and 77%, respectively. Among the 35 patients with chronic active hepatitis 6 (17%) were HBeAg-positive and 24 (69%) anti-HBe positive; among the 25 patients with chronic persistent hepatitis 3 (12%) were HBeAg-positive and 22 (88%) anti-HBe positive. Similar results were found in the groups of patients with cirrhosis and hepatoma. These findings show that all cases of acute hepatitis B were HBeAg-positive at the onset of the disease and a seroconversion to anti-HBe appeared very early in most of the cases. The extremely high incidence in particular of anti-HBe in the Greek patients with chronic liver diseases is in disagreement with the results of other studies from other populations. These differences may express differences in the immune response of the host in the different populations, or in the nature of the infecting strain commonly present in each country. Most of the HBsAg-negative patients with cirrhosis were found to be HBeAg or anti-HBe-positive.

Carcinoma, Hepatocellular↗
PubMed1982

Demonstration of alpha 1-antitrypsin in paraffin sections of hepatoma and cirrhosis.

Alpha 1-antitrypsin has been examined in formalin-fixed, paraffin-embedded liver specimens from Greek patients with cirrhosis (35 cases) and hepatoma (55 cases) by peroxidase-antiperoxidase (PAP) method. Ring-like AAT globules were found in the non-neoplastic cells in 12% of the cases of hepatoma and in 11% of the cases of cirrhosis. Atypical globules were seen in neoplastic cells in 5.4% of the cases of hepatoma and in 17% of the cases of liver cirrhosis. A diffuse fine granular pattern of AAT distribution was present in 31% of the cases of hepatoma in the neoplastic cells and in 27% of those in the non-neoplastic cells. The relatively high incidence of ring-like AAT-globules, and of atypical globules in cases of hepatoma and cirrhosis is not in agreement with the extremely low gene frequency of Z allele in a Greek population of patients with cirrhosis and hepatoma. Thus, there is some doubt whether AAT-globules in the liver represent a histopathologic marker of genetically determined AAT deficiency. A relationship between AAT deposits and the degree of differentiation of hepatoma was noted in this series. AAT-positive cells were found in 55% of moderately differentiated, in 29% of highly differentiated and in 20% of poorly differentiated hepatomas.

Carcinoma, Hepatocellular↗
PubMed1980

Alphafetoprotein levels of liver cancer patients and controls in a European population.

Serum alphafetoprotein (AFP) levels were determined by radioimmunoassay for 80 patients with primary hepatocellular carcinoma (PHC), 40 with metastatic liver cancer (MLC), and 204 controls; all were Caucasians of Greek nationality. Among histologically confirmed PHC cases, 62% had more than 1000 International Units per millilitre (IU/ml) AFP. Only one case with MLC (3%) exceeded 1000 IU/ml AFP, but lower elevations were not uncommon (13%). Among controls, none exceeded 40 IU/ml. Hepatitis B surface antigen (HBsAg) was detected among 6% of 17 histologically confirmed PHC patients with AFP less than 100 IU/ml and 60% of 63 PHC patients with more than 100 IU/ml of AFP (p < 0.001). Control subjects positive for HBsAg had significantly higher AFP values compared to those negative for it (P < 0.01) and male controls had slightly higher AFP values than female controls.

Carcinoma↗
PubMed1980

Serum Gm system in liver cirrhosis and hepatoma.

Serum Gm polymorphism was studied in 69 patients with liver cirrhosis, in 64 with liver cirrhosis plus hepatoma, in 40 with hepatoma without evidence of cirrhosis, and in 256 controls. The distribution of Gm factors in liver patients differed from that in the control group, this difference apparently being due to the distribution in cirrhosis patients negative for HBsAg and anti-HBs. Furthermore, significantly more heterozygous Gm individuals were found in this group of patients than in the control group. Thus it appears that Gm heterozygous individuals are prone to develop cryptogenic cirrhosis under the influence of other, as yet undetermined factors.

Carcinoma, Hepatocellular↗
PubMed1979

Detection of hepatitis B surface antigen in fixed tissues of patients with cirrhosis and hepatoma.

HBsAg has been sought by light microscopy in liver specimens from patients with cirrhosis (79 cases) and hepatoma (99 cases). The study was carried out on fixed material using orcein staining, immunoperoxidase technique and indirect immunofluorescence. HBsAg was detected in the serum by radio-immunoassay (RIA) using Ausria II-125 in 38 patients with cirrhosis and in 36 with hepatoma. In the 38 seropositive cases of cirrhosis HBsAg-positive cells were observed in 31 (81.6%) by the orcein staining and in 32 (84.2%) by the peroxidase and immunofluorescence staining. Among the 36 seropositive patients with hepatoma, HBsAg was detected in the surrounding non-neoplastic part of the liver, cirrhotic or not, in 30 (83.3%) by orcein staining and in 34 (94.4%) by the immunoperoxidase method and immunofluorescence. Positive solitary-cells were seen occasionally in the tumor tissue in 16 cases using orcein, in 9 using peroxidase and in 7 by fluorescence, out of the 36 seropositive patients with hepatoma. The results of this study do not support the hypothesis of a direct oncogenic effect of HBsAg on the liver cells, since this antigen was detected mainly in the non-neoplastic part of the liver tissue and only occasionally in the tumor cells. Of the 63 cases of seronegative hepatoma, 3 showed some round orcein-positive inclusion bodies in the cytoplasm of the neoplastic and the non-neoplastic cells; these bodies were not stained by the two immunological methods.

Carcinoma, Hepatocellular↗
PubMed1979

Serum trypsin inhibitory capacity and alpha 1-antitrypsin levels in liver cirrhosis and hepatoma.

alpha 1-Antitrypsin levels were measured in sera of 134 patients with cirrhosis and in 64 with cirrhosis and hepatoma. S-TIC was determined in 105 patients with cirrhosis and in 54 with cirrhosis and hepatoma. The mean alpha 1-at and S-TIC values in patients with cirrhosis were 369.59 +/- 14.072 mg% and 1,808 +/- 0.05 mg/ml respectively. In patients with cirrhosis and hepatoma the mean alpha 1-at level was 406.595 +/- 17.834 mg% and the S-TIC mean values was 2.064 +/- 0.82 mg/ml. Although these values are higher than those found in the healthy controls, the differences are not statistically significant.

Carcinoma, Hepatocellular↗
PubMed1979

Serum gastrin concentrations in healthy males and females of various ages.

We measured, by the RIA method, the concentrations of serum gastrin in a fasting state in 80 healthy volunteers of both sexes (M = 43, F = 37) aged 15-81 years. We did not find significant differences in the mean values of gastrin among them and the correlation of gastrin with the ages was insignificant. Further study of various age groups (15-29, 30-59, greater than 60 years) in both sexes and of two more age groups (18-45, 46-71) in the females, did not disclose any significant differences either. People of ages greater than 60 years had the greatest concentration of gastrin. Furthermore, we studied the concentrations of gastrin in the sera of 39 of these 80 people (M = 23, F = 16), aged 15-47 years, 10 min. and 40 min. after a test meal. No differences were found between the two sexes. However, although the mean levels of gastrin were significantly increased 10 min and 40 min after the meal in comparison with the fasting levels in both sexes, only in the men was the increase after 40 min. significantly greater than that after 10 min.

Adolescent↗
PubMed1978

Serum gastrin concentrations in healthy people of the various ABO blood groups.

The concentrations of gastrin were measured by the RIA method in the sera of 121 healthy Greek volunteers of both sexes and of different ABO blood groups, aged 20--70 years. The determinations took place in a fasting state in all the individuals and 10 min and 40 min after a test meal in 42 of them. No significant differences were found in the mean concentrations of gastrin in the fasting state and after the meal between the various ABO groups. There was an increase in the mean concentrations of gastrin in all the groups after the meal. Nevertheless, this was significant 40 min after the meal in the groups A and B whilst in the group O and in the groups A, B and AB considered together this significant increase had appeared already 10 min after the meal.

ABO Blood-Group System↗
PubMed1977

Serum haptoglobin phenotypes and duodenal ulcer.

Serum haptoglobin phenotypes were studied in 100 Greek patients suffering from duodenal ulcer by vertical starch gel electrophoresis. A sample of 2026 healthy subjects served as control. No statistically significant differences were found in Hp phenotypes and gene frequencies between patients and healthy controls.

Duodenal Ulcer↗
PubMed1976

Alpha 1-antitrypsin phenotypes in cirrhosis and hepatoma.

The phenotypic distribution of alpha-1-antitrypsin variants has been studied in 101 patients with cirrhosis and in 50 with cirrhosis plus hepatoma. 504 healthy Greeks served as controls. The ZZ and MZ phenotypes were found only once in the group of cirrhosis. The very low PiZ gene frequency suggests that the association of PiZ gene with cirrhosis is fortuitous. The FM phenotype has been observed in 14% of patients with hepatoma arised on cirrhosis and this incidence differed significantly between the two groups of patients and the controls. It is possible that cirrhotic patients phenotypically FM develop for as yet unknown reasons hepatoma in high percentage.

Carcinoma, Hepatocellular↗
PubMed1975

The Gm and Inv factors in rheumatoid arthritis.

The Gm(1), Gm(2), Gm(4), Gm(12), and Inv (1) factors were studied in the sera of 56 patients suffering from rheumatiod arthritis and 26 from various rheumatic diseases, by the hemagglutination inhibition test, using optimally reacting mixtures of Ragg and Nagg sera. The distribution of these factors was found to agree with that of healthy Greeks. No correlation was found between hypergammaglobulinemia and the discovery of the Gm(1) and Inv (1) factors. The presence of the rheumatoid factor was independent of the Gm and Inv phenotypes.

Arthritis, Rheumatoid↗