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Biomedical subjects

G T Sukhikh

Publications and source records attributed to G T Sukhikh.

At least 19 recordsLinked to original sources
PubMed1999

[Analysis of peptides from animal and plant tissues].

A new technique was developed for the analysis of peptide compositions of extracts from various animal and plant tissues. It involves the acidic extraction of a peptide fraction from the starting material and its precipitation with acetone, fractionation of peptides by ion-exchange chromatography by using a stepwise elution of fractions and detection by means of ninhydrin color reaction, and computer processing of the results. For the presentation of the results of analysis, chromatographic profiles and peptidograms were proposed. The results of analysis can be stored in a database and used for the creation of "generalized peptide portraits" and "differential peptide portraits" of the subjects investigated, which allow the identification of peptides characteristic of the subjects. The amount of peptide undergoing analysis ranged from 1 to 10 nmol.

Animals↗
PubMed1999

[Lipoprotein oxidation in the blood of women with pregnancy complications].

The level of lipoprotein oxidation in blood sera of pregnant women with obstetrical abnormalities is studied. Lipid peroxides were measured by a modified thiobarbituric acid test. The level of lipoprotein oxidation was increased, which may be one of the pathogenetic mechanisms of vascular disease in fetoplacental insufficiency and gestosis.

Adult↗
PubMed1998

Evaluation of stimulus-induced acrosome reaction by two-colour flow cytometric analysis.

Acrosome status in human spermatozoa from 20 normozoospermic men was evaluated by flow cytometry following the induction of the acrosome reaction with the ionophore A23187. Dual fluorescence staining of methanol fixed spermatozoa incubated with and without (control) the ionophore A23187 was performed with probes which targeted the outer acrosomal membrane (OAM) (rhodamine-labelled Arachis hypogaea agglutinin) or constituents of the acrosomal vesicle (fluorescein-labelled Pisum sativum agglutinin). Flow cytometry analysis revealed two major subpopulations of cells: acrosome-intact and acrosome-reacted spermatozoa after induction of the acrosome reaction. The intensity of green and red fluorescence in acrosome-reacted spermatozoa was significantly lower than that of the acrosome-intact control spermatozoa (P < 0.0001). The intensity of green fluorescence in the acrosome-intact subpopulation of spermatozoa was significantly higher than that of the control population (P < 0.002). Exposure of spermatozoa to the ionophore A23187 resulted in reliable enhancement of the number of spermatozoa with very high intensity of green and/or red fluorescence compared with the control (P < 0.03). An inverse correlation between the number of acrosome-reacted spermatozoa and spermatozoa with a very high intensity of green and/or red fluorescence was demonstrated (r = -0.631, P < 0.01). This method provides an objective and efficient procedure for quantitative estimation of the acrosomal status of human spermatozoa.

Acrosome↗
PubMed1998

[THe use of a biotin-labelled DNA probe for detecting the causative agents of chronic urogenital infections].

DNA hybridization for detecting HSV, CMV, C. trachomatis, and U. urealyticum by biotin-labeled DNA probe was used for investigating clinical specimens from patients with infertility and chronic urogenital inflammations. High sensitivity and specificity of the method was confirmed by the results of PCR, ELISA, and immunofluorescent methods in 80-90% cases. DNA hybridization technique is a simple method requiring no sophisticated equipment, which recommends it for the diagnosis of sexually-transmitted diseases at clinical laboratories.

Biotin↗
PubMed1997

Flow cytometry study on the effect of serum and peritoneal fluid of women on sperm-binding activity of immunoglobulin G antisperm antibodies.

OBJECTIVE: To investigate the influence of sera and peritoneal fluids (PFs) from fertile and infertile women on the binding of antisperm antibodies to the surface of spermatozoa. DESIGN: The immunoglobulin (Ig) G antisperm antibodies binding to the surface of liver spermatozoa was evaluated after their incubation in antisperm antibodies-positive serum from an infertile male in the presence and absence of female sera or PFs. SETTING: Russian Scientific Center for Obstetrics, Gynaecology, and Perinatology. PATIENT(S): Serum and PF samples from fertile and infertile women; antisperm antibodies-positive serum from infertile men; high-quality fresh semen from healthy donors. INTERVENTION(S): Serum samples were obtained from fertile and infertile women and from infertile men. Peritoneal fluids were collected during routine laparoscopy. MAIN OUTCOME MEASURE(S): The proportion of spermatozoa positive for IgG antibodies and the quantity of antisperm antibodies on the sperm surface measured by flow cytometry (FCM). RESULT(S): The addition of sera from fertile or infertile women with endometriosis or pelvic adhesion disease to an IgG antisperm antibodies-positive male serum resulted in significant inhibition of the antisperm antibodies binding to the sperm surface. CONCLUSION(S): Sera of fertile as well as infertile woman contain factors that block IgG antisperm antibodies binding to the surface of live spermatozoa.

Ascitic Fluid↗
PubMed1997

[Immunoenzyme analysis in the determination of antibodies to main classes of cell phospholipids in human blood].

A modified ELISA is proposed for detecting antiphospholipid antibodies of the IgM and IgG isotypes, including antibodies to cardiolipin, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, and sphingomyelin in human blood serum. The degree of phospholipid saturation, its phase (lamellar or hexagonal), purity, oxidation index of the phospholipid preparation, and conditions of solid phase treatment are important factors providing a sufficiently high sensitivity and reliability of measurements. The clinical value of the method was demonstrated in examinations of 70 patients with habitual abortions. The method can be used in studies of the spectrum of antiphospholipid antibodies in different diseases involving an increase of their blood serum level.

Adult↗