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Biomedical subjects

G T Golden

Publications and source records attributed to G T Golden.

95 records · Page 6Linked to original sources
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Infant rats: hypothalamic unit activity.

Single-unit recordings were made from lateral hypothalamic area (LHA) neurons of infant (8 through 21 days of age) male and female albino rats. Basal (or spontaneous) firing rates of LHA neurons were recorded for 20 min from rats 8 through 21 days of age, and mean basal firing rates did not differ across ages. Basal firing rates of LHA neurons in infant rats ranged from less than 1 spike/sec up to 27 spikes/sec, with 56% of the basal rates less than 1 spike/sec. Infant rats also received hypertonic saline injections (and control injections) to determine osmosensitivity characteristics of LHA neurons. Across ages, 56%--88% of LHA neurons were osmosensitive, with the highest percentages of osmosensitive units found in rats 14--21 days of age. Infant rats showed LHA unit electrophysiological characteristics that were quite similar to those obtained from LHA neurons in adult rats with respect to basal firing rates and osmosensitivity, however, LHA neuron activity in infants was not modulated by sensory stimuli as is frequently reported for adults. The results are discussed in terms of functional development of the LHA.

Action Potentials↗
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Ontogeny of drinking behavior of preweanling rats with lateral preoptic damage.

Male (N = 9) and female (N = 7) infant albino rats sustained bilateral lateral preoptic area (LPO) destruction on the tenth day of life. During development and as adults, these brain-damaged rats were subjected to repeated testing of drinking behaviors in response to cellular and extracellular dehydration. Immediately following LPO destruction the pups ceased suckling and most rats required tube-feeding to maintain life; however, all pups were maintaining body weight through voluntary suckling and/or feeding-drinking by 20-21 days of age. Daily water intakes were elevated from 30-42 days of age, following which intakes decreased to control vonse to water deprivation, polyethylene glycol injection and food deprivation treatments. These results are consistent with the suggestion that the LPO contains osmoreceptors for drinking behavior, and the present results suggest that such osmoreceptors, may be functional by the tenth day of life for rats.

Animals↗
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Potentiation of kainic acid epileptogenicity and sparing from neuronal damage by an NMDA receptor antagonist.

The time course and severity of the excitotoxic syndrome induced in rats by s.c. injection of 10 mg/kg kainic acid (KA) was modified by pretreatment with MK801, a non-competitive inhibitor of the NMDA receptor, at doses of 0.1, 1 and 10 mg/kg. A dose-dependent increase in the severity of the KA-induced electrographic (EEG) manifestations of epilepsy was seen after MK801. This consisted of an earlier appearance and higher number of EEG seizures, longer time spent in seizures, and an earlier onset of status epilepticus. In contrast, behavioral seizures were increased only in the 0.1 mg/kg MK801 group, but abolished by higher doses. On the contrary, wet dog shakes were progressively reduced with increasing doses of MK801. Four of the 9 animals receiving KA-only group and 3 of the 10 animals in the 1 and 10 mg MK801 groups were sacrificed 5 days after KA. The brain of the KA-only rats presented diffuse gross and microscopic evidence of hemorrhagic necrosis and neuronal damage; the MK801 rats showed only minimal neuronal loss in the CA3 hippocampal sector. This study demonstrates that neuronal damage and epileptiform activity can be dissociated. Furthermore, it confirms the protective effect of MK801 against neuronal damage caused by multiple factors. Lastly, it emphasizes the need for EEG monitoring in order to accurately assess any epileptic/antiepileptic effect.

Animals↗
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Neuropharmacological analysis of caudate-induced cortical inhibition.

The effects of cortical superfusion of various chemical agents on the inhibition of the discharge of cortical neurons evoked by caudate nucleus stimulation were studied in urethane anesthetized rats. The post-stimulus inhibition (PSI) was unaffected by cortical superfusion with bicuculline, 6-amino-methyl 3-methyl-4H, 1, 2, 6-benzothiadiazine-1, 1-diazidehydrochloride (TAG) and atropine. The PSI was readily and reversibly blocked by cortical superfusion or i.v. injection of strychnine independently of both strychnine capability of eliciting epileptiform activity, and of the degree of excitation produced by the drug. Two animals with poor or absent PSI showed appearance of PSI after glycine topical application. These data suggest that in the rat neocortex, glycine may mediate the inhibitory effect resulting from caudate stimulation.

Animals↗