[Non-A, non-B hepatitis transmitted by blood transfusion].
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Adult↗
Biomedical subjects
G T Espersen
Publications and source records attributed to G T Espersen.
Irradiated blood platelet concentrates stored for five days--evaluation by in vitro tests.
Platelet concentrates, irradiated with 15 Gy and stored for 5 days at room temperature under standardized conditions, were evaluated by in vitro tests and electron microscopy, in a paired study with nonirradiated platelets from the same concentrates, to investigate their usefulness for transfusion. The results showed no significant differences between the two groups in pH, pO2, pCO2, in vitro platelet aggregation, LDH, beta-thromboglobulin and thromboxane-B2. Examination by electron microscopy showed a higher degree of degranulation in the 5-day-old platelets but no certain difference between irradiated versus nonirradiated platelets. On the basis of satisfactory in vitro storage properties, platelet concentrates can be stored for 5 days in PL-1240 bags after irradiation. However, we recommend irradiation just before transfusion whenever possible.
Blood Platelets↗
Comparative studies on RF-IgA and RF-IgM ELISA--human or rabbit IgG as antigen?
In the literature it is seen that the antigens used in Elisa test for rheumatoid factors (RF) are either human IgG, rabbit IgG, or mouse IgG. We investigated RF-IgA activity in 55 serum samples using either human IgG or rabbit IgG as antigen. In other 76 serum samples we investigated RF-IgM activity using either human IgG or rabbit IgG as antigen. In RF-IgA the median AU/ml is found a factor 2-3 times higher using rabbit IgG as antigen. The discriminatory power of the RF-IgA Elisa test is better using rabbit IgG as antigen. In RF-IgM Elisa the results are different using human or rabbit IgG as antigen. Our investigation illustrates some of the problems in Elisa testing for RF's. We conclude that the variations are influenced by the antigen source, being rabbit or human IgG. This might affect the daily and scientific communication between different laboratories and/or clinical departments. Results obtained by one antigen cannot easily be compared with results obtained with the other antigen. This means that the user of the results has to be aware of the antigen used, and it seems most important to develop some kind of standardization in the Elisa tests used.
Animals↗
ELISA estimations of rheumatoid factor IgM, IgA, and IgG in sera from RA patients with high disease activity. DTT treatment studies.
Although Elisa assays detecting rheumatoid factor's (RF) show high sensitivity and specificity, difficulties with IgA- and especially IgG-RF testing in ELISA systems, due to interaction from 'contaminating' IgM-RF is still thought to be a problem. Sera from 15 Rheumatoid Arthritis patients with high disease activity and high IgM-RF values were Dithiothreitol (DTT) treated. IgM-RF values were reduced to approximately zero in all tested sera. IgA-RF activity declined as expected, but also showed a statistically significant correlation between % reduction after DTT treatment and the IgM-RF value from the same serum sample. IgG-RF also decreased after DTT treatment, most pronounced for high IgG-RF values. A correlation (not statistically significant) between the % reduction in IgG-RF after DTT treatment and the IgM-RF value from the same serum sample was observed. Pepsin and Diethylammonium ethyl (DEAE) reduced the IgG-RF activity even more than after DTT treatment of the sera. Fractionation by Gel filtration of 8 serum samples showed that all the RF activity were found according to the 'first top' of the gel filtration curve.
Arthritis, Rheumatoid↗
RF-classes (IgM, IgG, IgA) in a group of highly active RA-patients in relation to disease activity and treatment.
Rheumatoid factors IgM, IgG and IgA were measured in a group of highly active RA-patients before and after short time treatment with steroids. After 6 months treatment with DMARD measurements were carried out again, and RF-titres correlated to disease activity.
Arthritis, Rheumatoid↗
[NSAID (non-steroidal anti-inflammatory agent) and necrotizing fascitis].
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Adult↗
Passive tension of the ankle before and after stretching.
The passive tension resulting from dorsiflexion of the ankle was measured in relation to stretching in six handball players and six soccer players. Corresponding values of ankle angle and passive tension were measured by a strain gauge and a potentiometer connected to a pedal system. The passive tension versus ankle angle was measured before and 90 minutes after a single contract-relax stretching program of the plantar flexors. Stretching lowered the passive tension by up to 18%. Contract-relax stretching performed twice a day for 3 weeks lowered the passive tension in the plantar flexors by up to 36%. Before the last measurements, no stretching was performed for 20 hours or more. Stretching thus had both a short-term effect, matching the length of a training session, and a long-term effect, shown in a reduction of passive tension after 3 weeks. The relative decrease in passive tension after stretching exercises was constant from a neutral position of the ankle to maximal dorsiflexion. There was no correlation between 1) flexibility and the short-term effect of stretching, 2) flexibility and the long-term effect of stretching, or 3) the short-term and long-term effects of stretching. This indicates that passive tension was decreased in all subjects irrespective of their flexibility, and that subjects who had short-term effects after stretching did not necessarily demonstrate a long-term effect.
Adult↗