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G Stampacchia

Publications and source records attributed to G Stampacchia.

At least 37 records · Page 2Linked to original sources
PubMed1988

Inhibition of vestibulospinal reflexes following cholinergic activation of the dorsal pontine reticular formation.

1. The multiunit EMG activity of the forelimb extensor muscle triceps brachii was recorded in precollicular decerebrate cats, either at rest or during roll tilt of the animal at 0.15Hz, +/- 10 degrees leading to sinusoidal stimulation of labyrinth receptors. Both the spontaneous EMG activity as well as the labyrinthine-induced EMG responses were tested before and after pontine microinjection of a cholinergic agonist. 2. Local injection of the cholinergic agonist carbachol into the dorsal aspect of the pontine tegmentum (usually 0.25 microliter, 0.01-0.2 microgram/microliter) produced a state of postural atonia, and abolished both the spontaneous EMG activity as well as the EMG responses of the triceps brachii to sinusoidal stimulation of labyrinth receptors. This suppression was generally ipsilateral to the side of the injection and persisted throughout the episode of postural atonia, but sometimes it involved also the contralateral limbs. In these instances it could be accompanied by a spontaneous nystagmus, interspersed at regular intervals with bursts of rapid eye movements. 3. Similar effects were also obtained following injection of carbachol in the gigantocellular tegmental field (FTG) (0.25 microliter, 0.5-1.0 microgram/microliter). However, this structure was not critically responsible for the phenomena reported above, which persisted unaltered after kainic acid lesion of the FTG performed ipsilaterally to the side of the pontine injection. 4. Local infusion of the muscarinic blocker atropine sulphate reversed the effects of carbachol injection into the dorsal aspect of the pontine tegmentum, thus indicating that muscarinic receptors were involved. 5. It is postulated that the postural atonia as well as the tonic depression of vestibulospinal reflexes, which occur in the decerebrate cat after local injection of a cholinergic agonist depends, at least in part, on the activation of cholinoceptive neurons located in dorsal pontine reticular structures. These may in turn excite medullary reticulospinal neurons, which are finally responsible for the inhibition of extensor motoneurons.

Animals↗
PubMed1987

Effects of stimulation of vestibular and neck receptors on Deiters neurons projecting to the lumbosacral cord.

The activity of lateral vestibular nucleus (LVN) neurons, antidromically identified by stimulation of the spinal cord at T12 and L1, thus projecting to the lumbosacral segments of the spinal cord (IVS neurons), was recorded in precollicular decerebrate cats during rotation about the longitudinal axis either of the whole animal (labyrinth input) or of the body only while the head was kept stationary (neck input). Among the IVS neurons tested for vestibular stimulation, 76 of 129 units (i.e. 58.9%) responded to roll tilt of the animal at the standard parameters of 0.026 Hz, +/- 10 degrees. The gain and the sensitivity of the first harmonic responses corresponded on the average to 0.47 +/- 0.44, SD, impulses X s-1 X deg-1 and 3.24 +/- 3.15, SD, %/deg, respectively. As to the response patterns, 51 of 76 units (i.e. 67.1%) were excited during side-down and depressed during side-up tilt, whereas 15 (i.e. 19.7%) showed the opposite behavior. In both instances the peak of the responses occurred with an average phase lead of about +21.0 +/- 27.2, SD, deg with respect to the extreme side-down or side-up position of the animal. Moreover, the former group of units showed almost a twofold larger gain with respect to the latter group (t-test, p less than 0.05). Among the IVS neurons tested for neck stimulation, 75 of 109 units (68.8%) responded to neck rotation at the standard parameters. The gain and the sensitivity of the first harmonic responses corresponded on the average to 0.49 +/- 0.40, SD, impulses X s-1 X deg-1 and 3.30 +/- 3.42, SD, %/deg, respectively, thus being similar to the values obtained for the labyrinth responses. However, 59 of 75 units (i.e. 78.6%) were excited during side-up neck rotation and depressed during side-down neck rotation, while 8 of 75 units (i.e. 10.7%) showed the opposite pattern. In both instances the peak of the responses occurred with an average phase lead of +52.0 +/- 18.3, SD, deg for the extreme side-up or side-down neck displacements. Further, the former group of units showed a larger gain than the latter group. Histological controls indicated that 102 of 129 (i.e. 79.0%) IVS neurons tested for labyrinth stimulation and 86 of 109 (i.e. 78.9%) IVS neurons tested for neck stimulation were located in the dorsocaudal part of LVN, the remaining IVS neurons being located in the rostroventral part of LVN.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1987

Effects of roll tilt of the animal and neck rotation on different size vestibulospinal neurons in decerebrate cats with the cerebellum intact.

In decerebrate cats with the cerebellum intact we recorded the activity of lateral vestibulospinal neurons projecting to lumbosacral segments of the spinal cord (IVS neurons) and related the resting discharge, as well as the response characteristics of these neurons to roll tilt of the animal and neck rotation, with the cell size inferred from the conduction velocity of the corresponding axons. A slight negative correlation was found between resting discharge rate and conduction velocity of the whole population of IVS neurons responsive and unresponsive to animal tilt and neck rotation, so that the faster the conduction velocity, the lower was the unit discharge at rest. This correlation, however, was found only for the dorsocaudal LVN neurons, which contributed to the majority of IVS units, but not for the rostroventral LVN neurons. Moreover, it affected the units unresponsive but not those responsive to vestibular stimulation; the opposite, however, occurred for the units tested to neck stimulation. These findings indicate that the static properties of the IVS neurons can only in part be related to cell size. If we consider the IVS neurons responsive to roll tilt of the animal (76 neurons) and neck rotation (75 neurons) at the standard parameters of 0.026 Hz, +/- 10 degrees, no significant correlation was found between gain (impulses X s-1 X deg-1) of the labyrinth or neck responses and conduction velocity of the axons. Thus, due to the presence of slight negative relation between resting discharge and conduction velocity of the axons, larger neurons exhibited a greater percentage modulation (sensitivity) to the labyrinth and neck inputs than smaller neurons; this correlation involved particularly the dcLVN neurons. These findings suggest that the afferent pathways driven during dynamic stimulation of labyrinth and neck receptors produce an higher number or density of synaptic contacts on IVS neurons of increasing size. No significant differences in the means of resting discharge, conduction velocity, gain and sensitivity were found between all the IVS units responding to labyrinth and neck inputs. These findings indicate that the effectiveness of the two inputs was almost comparable and did not vary in different units as a function of cell size. The IVS neurons were mainly excited during side-down animal tilt and side-up neck rotation. Although these neurons showed the same spectrum of conduction velocity as those displaying the opposite response patterns, the response gains of the predominant populations of units were on the average higher than those obtained from the remaining populations of units.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1987

Pontine reticular origin of cholinergic excitatory afferents to the locus coeruleus controlling the gain of vestibulospinal and cervicospinal reflexes in decerebrate cats.

1. Previous experiments had shown that the medullary inhibitory reticulospinal (mRS) neurons act 180 degrees out-of-phase with respect to the excitatory vestibulospinal (VS) neurons during the vestibular and the neck reflexes involving the limb extensor motoneurons. This finding suggested that the higher the firing rate of the medullary inhibitory RS neurons in the animal at rest, the greater the disinhibition which affects the limb extensor motoneurons during side-down roll tilt of the animal or side-up neck rotation, thus leading to an increased gain of response of limb extensors to sinusoidal stimulation of labyrinth and neck receptors. The gain of these postural reflexes would then represent a sensitive test to evaluate the background discharge of the inhibitory reticulospinal system of the medulla. 2. The discharge of the inhibitory mRS neurons is under the tonic excitatory control of cholinergic pontine reticular formation (pRF) neurons which are also self-excitatory, while these cholinergic pontine neurons are in turn inhibited by the norepinephrine (NE)-containing locus coeruleus (LC) neurons, which are also self-inhibitory due to mechanisms of recurrent and/or lateral inhibition. The present experiments were performed to find out whether cholinergic and cholinoceptive pontine reticular neurons, which are under the inhibitory control of the LC neurons, also send axons to the LC on which they may exert an excitatory influence. This excitatory effect would then counteract the self-inhibitory influence mediated by the NE, which acts on the alpha 2-adrenoceptors distributed on the somatodendritic membrane of the LC neurons. 3. In precollicular decerebrate cats, local injection into the dorsal aspect of the pontine tegmentum of 0.25 microliter of a solution of the muscarinic blocker atropine sulphate at the concentration of 6 micrograms/microliter of sterile saline did neither modify the postural activity in the ipsilateral limbs nor the response gain of the ipsilateral forelimb extensor triceps brachii to sinusoidal stimulation of labyrinth receptors (roll tilt of the animal at 0.15 Hz, +/- 10 degrees). These negative results were attributed to the fact that in these preparations the activity of the cholinergic and cholinoceptive pRF neurons and the related inhibitory mRS neurons is very low, due to the tonic discharge of the NE-containing LC neurons, which exert a prominent inhibitory influence on the underlying reticular structures.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1987

Convergence of neck and macular vestibular inputs on vestibulospinal neurons projecting to the lumbosacral segments of the spinal cord.

The activity of LVN neurons was recorded in decerebrate cats and analyzed during separate stimulation of macular vestibular and neck receptors elicited by sinusoidal rotation about the longitudinal axis at 0.026 Hz, 10 degrees peak amplitude. Of 119 LVN units examined, the great majority, i.e. 106, were vestibulospinal neurons antidromically identified following stimulation of the spinal cord at T12-L1, thus projecting to the lumbosacral segments of the spinal cord (IVS neurons); the remaining 13 units were nonantidromically activated. Among the 119 LVN neurons, 77 (64.7%) responded with a periodic modulation of their firing rate to roll tilt of the animal and 81 (68.1%) responded to neck rotation. Convergence of macular and neck inputs was found in 58/119 (48.7%) lateral vestibular neurons; in these units, the gain as well as the sensitivity of the first harmonic of responses corresponded on the average to 0.58 +/- 0.45, S.D. imp./sec/deg and 4.39 +/- 3.58, S.D.%/deg for the neck responses and 0.52 +/- 0.49, S.D. imp./sec/deg and 3.85 +/- 3.35, S.D.%/deg for the macular responses, respectively. In addition to these convergent units, 19/119 (16.0%) and 23/119 (19.3%) lateral vestibular units responded to selective stimulation either of macular receptors or of neck receptors only. These units, which showed on the average an higher firing rate and a lower conduction velocity of the corresponding vestibulospinal axons than the convergent units, displayed a significantly lower response gain and sensitivity to animal tilt and neck rotation with respect to those obtained from convergent units. Most of the convergent lateral vestibular units were maximally excited by the direction of stimulus orientation, the first harmonic of responses showing an average phase lead of +51.4 degrees with respect to neck position and +21.9 degrees with respect to animal position. Two populations of convergent neurons were observed. The first group of units (53/58, i.e. 91.4%) showed reciprocal ("out-of-phase") responses to the two inputs in that they were mainly excited during side-down animal tilt and side-up neck rotation. The remaining group of units (5/58, i.e. 8.6%) showed parallel ("in phase") responses to the two inputs and they were mainly excited by side-up neck rotation and animal tilt. Interestingly, the former group of units displayed an average gain and sensitivity to the labyrinth and neck inputs which were more than twice higher than the values obtained from the latter group of units.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1987

Effects of microinjection of a cholinergic agonist into the locus coeruleus on the gain of vestibulospinal reflexes in decerebrate cats.

1. Experiments were performed in precollicular decerebrate cats to determine whether activation of locus coeruleus (LC) neurons elicited by local injection of the cholinergic agonist carbachol modifies the dynamic characteristics of responses of forelimb extensors to selective stimulation of labyrinth receptors resulting from roll tilt of the animal. 2. Injection of 0.1-0.4 microliter (usually 0.25 microliter) of carbachol at a concentration of 0.02-0.1 micrograms/microliter of sterile saline into the LC of one side, which slightly increased the tonic contraction of limb extensors ipsilateral to the side of the injection, greatly decreased the amplitude of the multiunit EMG response of the ipsilateral triceps brachii to animal tilt at 0.15 Hz, +/- 10 degrees. Correspondingly, the response gain of this forelimb extensor decreased. Moreover, a significant increase in phase lag of the responses was observed. These findings did not result from the increased postural activity, since they were still observed when the limb position was adjusted so that the spontaneous EMG activity remained constant throughout the experiments. 3. The changes in posture as well as in response characteristics of the forelimb extensor to labyrinth stimulation produced by carbachol injection appeared a few min after the injection and soon reached a plateau level which persisted for several hours before returning to the control levels. 4. The effects described above involved mainly, if not exclusively, the limbs ipsilateral to the side of the injection. However, the effects of local injection into the LC of one side could be reproduced on the contralateral side following injection into the LC of that side. 5. The increase in phase lag of the multiunit EMG responses of the triceps brachii to labyrinth stimulation appeared at a threshold lower than that required to decrease the response gain of this extensor muscle. These findings suggest that different neuronal populations within the LC complex, one projecting directly to the spinal cord, the other projecting indirectly through the pontine reticular formation, are involved in the control of phase angle and gain of the vestibulospinal reflexes, respectively. However, as soon as the threshold was reached the effects described above were dose-dependent. 6. Histological controls indicated that the structure responsible for the postural and reflex changes described above corresponded to the LC. In fact, postural and reflex changes opposite in sign to those described above were obtained when the same amount of carbachol was injected into the dorsal aspect of the pontine reticular formation (pRF) located immediately ventral to the LC.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1987

Effects of microinjection of cholinergic agonists into the pontine reticular formation on the gain of vestibulospinal reflexes in decerebrate cats.

1. The question of which pontine neuronal groups and related receptors can mediate the cholinergic induction of the increased gain of vestibulospinal reflexes elicited by sinusoidal stimulation of labyrinth receptors was investigated by injecting in precollicular decerebrate cats either carbachol, which is a mixed muscarinic-nicotinic agonist, or bethanechol, which is a pure muscarinic agonist, via a cannula stereotaxically oriented in different pontine tegmental structures. 2. Injection of 0.1-0.2 microliter of carbachol solution (0.01-0.2 microgram/microliter of sterile saline) into the dorsal aspect of the pontine reticular formation (pRF), which slightly decreased the tonic contraction of limb extensors ipsilateral to the side of the injection, greatly increased the amplitude of the multiunit EMG response of the ipsilateral triceps brachii to roll tilt of the animal at 0.15 Hz, +/- 10 degrees, leading to selective stimulation of labyrinth receptors. Correspondingly, the response gain of the forelimb extensor to labyrinth stimulation increased. Moreover, a slight decrease in phase lead of the responses was observed. These findings were not attributable to decreased postural activity, since they were still observed when postural EMG activity was reflexly maintained by an increased static stretch of the muscle. No changes in the dynamic characteristics of the responses were observed in the contralateral triceps brachii. 3. The changes in posture as well as in response gain produced by the carbachol injection appeared suddenly, but partially declined to reach a plateau level which persisted for several hours before returning to the control level. Moreover, the magnitude of the effects increased in relation to the dose of the cholinergic agonist. 4. Histological controls indicated that the structure responsible for these postural and reflex changes was located in the dorsal aspect of the pontine tegmentum immediately ventral to the principal locus coeruleus (LC); this area corresponds to the peri-LC region and the surrounding pRF including the dorsal aspect of the central tegmental field. The effects were still obtained after chronic kainic acid lesioning of the gigantocellular area of the medulla. 5. An increase in gain of the vestibulospinal reflex which was as potent, dose-dependent, and site-specific as that previously observed with carbachol, appeared after injection of the pure muscarinic agonist bethanechol.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1986

Effects of pressure stimulation of the body surface on posture and vestibulospinal reflexes.

The effects of pressure stimulation of the body surface on postural activities as well as on the response gain of limb extensors to natural stimulation of labyrinth receptors were investigated in intact, as well as in decerebrate cats. In intact, unanesthetized cats, slight pressure applied symmetrically to the body surface at the chest level decreased the tonic activity of the axial (neck) and limb extensor musculature, as well as the proprioceptive reflexes induced by passive flexion of the limbs. The positive supporting reaction caused by pressure applied to the pad of the foot was also depressed. If the cats were suspended in the air by their nape, slight pressure applied to the upper part of the body greatly reduced the tonic contraction of the forelimb extensors to linear acceleration after downward movement of the animal, a response which can be attributed to stimulation of macular receptors located in the sacculus. Moreover, the prominent myotatic reflexes which occurred in all four limbs as soon as the animal touched the floor were greatly depressed, as shown by the fact that the forelimbs displayed only a slight tonic contraction of the extensor musculature during landing, while the hindlimbs collapsed under the weight of the body. In precollicular decerebrate cats there was a good postural activity in all four limbs. Moreover, the multiunit EMG activity of the medial head of the triceps brachii responded to roll tilt of the animal (at 0.15 Hz, +/- 10 degrees) leading to selective stimulation of labyrinth receptors. These responses, characterized by an increased EMG activity during side-down tilt and a decreased activity during side-up tilt, were related to animal position and not to velocity of animal displacement, and are thus attributable to stimulation of macular, utricular receptors. Slight pressure applied to the chest greatly decreased not only the postural activity of the limbs, but also the amplitude of EMG modulation and then the gain in the first harmonic component of the multiunit EMG responses of the triceps brachii to animal tilt. This reduced gain was due, in particular, to a reduced number of motor units being recruited during labyrinth stimulation, although a reduced modulation of firing rate of the active motor units should not be ruled out. However, no changes in the phase angle of the responses were observed.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1985

Effects of passive flexion of the forepaw on the response gain of limb extensors to sinusoidal stimulation of labyrinth receptors.

The main aim of the present study was to find out whether the dynamic characteristics of responses of limb extensor muscles to labyrinth stimulation were modified by the proprioceptive input elicited by appropriate displacements of the corresponding limb extremity. In cats decerebrated at precollicular or intercollicular level, the multiunit EMG activity of the medial head of the triceps brachii was recorded during roll tilt of the animal at the frequency of 0.15 Hz, +/- 10 degrees leading to selective stimulation of labyrinth receptors. This stimulation was then tested several times at regular intervals of 2 to 6 min for several hours while maintaining the ipsilateral forelimb in the horizontal extended position, i.e. with the plantar surface of the foot lying on the tilting table, or during passive flexion of the forepaw in plantar or dorsal direction. In all the experiments in which the forelimb was in the control position, the multiunit EMG responses of the triceps brachii were characterized by an increased activity during side-down tilt of the animal and a decreased activity during side up tilt. These responses were related to animal position and not to the velocity of animal displacement, thus being attributed to stimulation of macular, utricular receptors. Static displacement of limb extremities following plantar flexion of the forepaw greatly decreased the amplitude of the EMG modulation and thus the gain of the first harmonic component of the multiunit EMG responses of the ipsilateral triceps brachii to animal tilt. This reduced gain was due not only to a reduced number of motor units recruited during labyrinth stimulation, but also to a reduced modulation of firing rate of the active motor units, as shown by recording the activity of individual motor units. On the other hand, displacement of the same extremity in the opposite direction, i.e. following dorsiflexion of the forepaw, enhanced the amplitude of the EMG modulation and thus the gain of the multiunit EMG responses of the ipsilateral triceps brachii to animal tilt. This finding was mainly due to an increased recruitment of motor units during side-down tilt, although an increased modulation of the firing rate of individual motor units could not be excluded. In both instances, no changes in the phase angle to the responses were observed. The changes in response gain described above depended on the amount of passive displacement of the forepaw and persisted unmodified throughout the new maintained position.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1984

Responses of medullary reticulospinal neurons to sinusoidal rotation of neck in the decerebrate cat.

The electrical activity of 132 neurons located in the inhibitory area of the medullary reticular formation, namely, in the medial aspects of the nucleus reticularis gigantocellularis, magnocellularis and ventralis has been recorded in precollicular decerebrate cats during sinusoidal displacement of the neck. This was achieved by rotation of the body about the longitudinal axis of the animal, while maintaining the head stationary. In particular, 85 neurons were activated antidromically by stimulation of the spinal cord at T12 and L1, the remaining 47 units were not activated antidromically. Among these reticular neurons tested, 66 out of 85 (i.e. 77.6%) of the neurons that were, and 31 out of 47 (i.e. 66.0%) of the neurons that were not antidromically activated responded to slow neck rotation at the frequency of 0.026 Hz and at the peak amplitude of displacement of 10 degrees. The units influenced by neck rotation showed a periodic modulation of the firing rate in response to sinusoidal stimulation of neck receptors. In particular, 70 of 97 units (i.e. 72.2%) were excited during side-down neck rotation and depressed during side-up rotation, while 19 of 97 units (i.e. 19.6%) showed the opposite pattern. In both instances, the peak of the responses occurred with an average phase lead of +41 degrees for the extreme side-up or side-down neck displacement. The remaining 8 units (i.e. 8.2%) showed a prominent phase shift of the peak of their response relative to neck position. The proportion of units excited during side-down neck rotation were almost equally distributed throughout the whole rostro-caudal extent of the reticular structures explored. Responses to neck rotation were detectable at 0.25 degrees of peak displacement. The gain (imp./s/deg.) and the sensitivity (%/deg., i.e. percentage change of the mean firing rate per degree of displacement) in responses of reticulospinal neurons decreased by increasing the peak amplitude of neck rotation from 1 to 10 degrees at a frequency of 0.026 Hz. Therefore, the system did not behave linearly with respect to amplitude of stimulation. By increasing the frequency of stimulation from 0.008 to 0.32 Hz at the fixed amplitude of 10 degrees, the gain, sensitivity and phase lead of responses increased for frequencies of neck rotation above 0.051 Hz. Reticulospinal neurons may thus monitor changes in neck position as well as in velocity of neck rotation. Responses of reticulospinal neurons to neck rotation are discussed in relation to the responses to the same stimulus recently described of vestibulospinal neurons originating from the lateral vestibular nucleus.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1984

Convergence and interaction of neck and macular vestibular inputs on reticulospinal neurons.

Extracellular recordings were obtained in decerebrate cats from neurons located in the inhibitory area of the medullary reticular formation, namely in the medial aspects of the nucleus reticularis gigantocellularis, magnocellularis and ventralis. Of 127 medullary reticular units examined, 77 were reticulospinal neurons antidromically identified following stimulation of the spinal cord at T12-L1; the remaining 50 neurons were not activated antidromically. Unit firing rate was analyzed under separate stimulation of macular vestibular, neck, or combined receptors by using sinusoidal rotations about the longitudinal axis at 0.026 Hz, 10 peak amplitude. Among the 127 reticular units, 84 (66.1%) responded with a periodic modulation of their firing rate to roll tilt of the animal and 93 (73.2%) responded to neck rotation. Convergence of macular and neck inputs was found in 71/127 (55.9%) reticular neurons; in these units, the gain as well as the sensitivity of the first harmonic of response corresponded on the average to 0.49 +/- 0.41, SD imp/s/deg and 5.10 +/- 4.27, SD %/deg for the neck responses and to 0.40 +/- 0.39, SD imp/s/deg and 3.90 +/- 3.80, SD %/deg for the macular responses, respectively. Most of the convergent reticular units were maximally excited by the direction of stimulus orientation, the first hormonic or responses showing an average phase lead of about +42.7 with respect to neck position and +24.9 with respect to animal position. Two populations of convergent neurons were observed. The first group of units (58/71, i.e. 81.7%) showed reciprocal ("out of phase") responses to the two inputs in that they were mainly excited during side-down neck rotation, but inhibited during side-down animal tilt. The remaining group of units (13/71, i.e. 18.3%) showed parallel ("in phase") responses to the two inputs and they were mainly excited by side-down neck rotation and animal tilt. The response characteristics of medullary reticular neurons to the combined neck and macular inputs, elicited during head rotation, closely corresponded to those predicted by a vectorial summation of the individual neck and macular responses. In particular, "out of phase" units displayed small amplitudes and large phase leads of the responses with respect to head position, when both types of receptors were costimulated. In contrast, "in phase" units displayed large amplitude and small phase leads during head rotation.(ABSTRACT TRUNCATED AT 400 WORDS)

Afferent Pathways↗
PubMed1983

Relation between cell size and response characteristics of medullary reticulospinal neurons to labyrinth and neck inputs.

The activity of presumably inhibitory reticulospinal neurons with cell bodies located in the medial aspects of the medullary reticular formation and axons projecting to lumbosacral cord has been recorded in decerebrate cats and their response characteristics to sinusoidal stimulation of labyrinth receptors (134 neurons) and neck receptors (110 neurons) have been related to cell size inferred from the conduction velocity of the corresponding axons. No significant correlation was found between resting discharge and conduction velocity of the axons. Among the recorded reticulospinal neurons, 64/134 (i.e. 47.8%) units responded to roll tilt, while 66/110 (i.e. 60.0%) units responded to neck rotation (0.026 Hz, +/- 10 degrees). A positive correlation was found between gain (imp./s/deg) of the labyrinth and neck responses and conduction velocity of the axons. Thus, due to absence of correlation between resting discharge and conduction velocity of the axons, larger neurons exhibited a greater percentage modulation (sensitivity) to the labyrinth and the neck input than smaller neurons. These findings are attributed to an overall increase in density or efficacy of the synaptic contacts made by the vestibular and neck afferent pathways on reticulospinal neurons of increasing size. Units receiving neck-macular vestibular convergence showed on the average an higher gain of the neck (GN) response with respect to the labyrinth (GL) response (GN/GL: 1.95 +/- 1.49, S.D.; n = 43); however, due to a parallel increase in gain of the reticulospinal neurons to both neck and labyrinth inputs, the relative effectiveness of the two inputs did not vary in different units as a function of cell size. The reticulospinal neurons were mainly excited by the direction of animal orientation and/or neck displacement. In particular, most of these positional sensitive units were excited by side-up animal tilt (37/58, i.e. 63.8%) and by side-down neck rotation (47/60, i.e. 78.3%). These predominant response patterns were particularly found between large size neurons, whereas small size neurons tended to show also other response patterns. The evidence indicates that in addition to intrinsic neuronal properties related to cell size, the quantitative and qualitative organization of synaptic inputs represents the critical factor controlling the responsiveness of reticulospinal neurons to vestibular and neck stimulation.

Afferent Pathways↗
PubMed1983

Responses of medullary reticulospinal neurons to sinusoidal stimulation of labyrinth receptors in decerebrate cat.

The electrical activity of 168 individual neurons located in the medullary reticular formation, namely, in the medial aspects of the nucleus reticularis gigantocellularis, magnocellularis, and ventralis, has been recorded in precollicular decerebrate cats during sinusoidal tilt about the longitudinal axis of the whole animal, leading to stimulation of labyrinth receptors. In particular, 93 neurons were activated antidromically by stimulation of the spinal cord at T12 and L1 (1RS neurons); the remaining 75 neurons were not activated antidromically (RF neurons). Among these medial reticular neurons tested, 64 of 93 (i.e., 69%) 1RS neurons and 49 of 75 (i.e., 65%) RF neurons responded to slow rotation of the animal at the standard frequency of 0.026 Hz and at the peak amplitude of displacement of 10 degrees. A periodic modulation of firing rate of the units was observed during the sinusoidal stimulus. In particular, 71 of 113 units (i.e., 63%) were excited during side-up and depressed during side-down tilt, whereas 24 of 113 units (i.e., 21%) showed the opposite behavior. In both instances, the peak of the responses occurred with an average phase lead of about +25 degrees with respect to the extreme side-up or side-down position of the animal. The remaining 18 units (i.e., 16%) showed a prominent phase shift of the peak of their response with respect to animal position. Within the explored region of the medulla, the proportion of units excited during side-up tilt was higher at caudal levels, whereas that of the units excited during side-down tilt was higher at rostral levels. Units displaying intermediate phase angle of the responses predominated at intermediate levels of the medulla. Responses to animal tilt were detectable at 1 degree of peak displacement. The gain (impulses x s-1 x deg-1) of the responses of reticulospinal neurons did not change by increasing the peak amplitude of tilt from 5 to 20 degrees at the fixed frequency of 0.026 Hz. This finding indicates that the system was relatively linear with respect to the amplitude of displacement. By varying the frequency of stimulation from 0.008 to 0.32 Hz at the fixed amplitude of 10 degrees, two populations of reticulospinal neurons were observed. In the first, the gain and the phase angle of response remained relatively unmodified against changes in frequencies: these positional responses were attributed to stimulation of macular receptors.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1983

Inhibition of vestibular and neck reflexes in forelimb extensor muscles during the episodes of postural atonia induced by an anticholinesterase in decerebrate cat.

In precollicular decerebrate cats, the multiunit EMG activity of forelimb extensor muscles (e.g. the triceps brachii) was recorded during sinusoidal stimulation of labyrinth and neck receptors at the frequencies of 0.026-0.15 Hz, +/- 10 degrees and the resulting responses were tested during tonic activation of a cholinergic mechanism. In agreement with previous findings, the first harmonic component of the EMG responses to roll tilt of the animal leading to selective stimulation of labyrinth receptors was characterized by an increased activity during side-down tilt and a decreased activity during side-up tilt (labyrinth responses); on the other hand just the opposite changes were elicited for the same directions of neck rotation (neck responses). For the parameters of stimulation reported above, the responses were always related to position and not to velocity of displacement. Intravenous injection of an anticholinesterase (eserine sulphate, 0.10-0.15 mg/kg) which produced a state of postural atonia, associated with bursts of rapid eye movements (REM), similar to that occurring spontaneously in unrestrained cats during desynchronized sleep or REM sleep, also decreased the tonic activity of the triceps brachii and abolished the EMG responses of this muscle to sinusoidal stimulation of labyrinth and neck receptors. This suppression persisted throughout the episode of postural atonia associated with REM bursts. The abolition of the labyrinth and neck reflexes acting on forelimb muscles was not only dependent on the dose of anticholinesterase, but also on the state of the animal. In fact, somatosensory or acoustic stimuli applied during the REM episodes abolished the rhythmic oculomotor activity and determined the prompt recovery of both the decerebrate rigidity and the EMG responses of the triceps brachii to labyrinth and neck stimulation. The postural atonia as well as the tonic depression of the vestibular and neck reflexes acting on forelimb extensor muscles can in part at least be attributed to cholinergic activation of medullary reticulospinal neurons exerting a postsynaptic inhibitory influence on extensor motoneurons. However, since these inhibitory reticulospinal neurons collaborate with excitatory vestibulospinal neurons to the motoneuronal responses during stimulation of labyrinth and neck receptors, we cannot exclude that the suppression of the vestibular and neck reflexes may also depend on occlusion of the unit responses at reticular level.

Animals↗