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Biomedical subjects

G Spoto

Publications and source records attributed to G Spoto.

47 records · Page 3Linked to original sources
PubMed1976

A study of the EEG sleep patterns and the sleep and dream experience of a group of schizophrenic patients treated with sulpiride.

The modifications of spontaneous sleep in schizophrenic patients following N-ethyl-2(2-methoxy-5-sulfamido-benzamidomethyl)-pyrrolidine (sulpiride, Dobren) administration are reported in this study. No significant modifications in the quantitative distribution of the various sleep phases (LST, SWS, REM) were observed, while a significant percent increase of SWS was noted, together with a significant decrease in the number of awakenings during the night and an increase of the sleep onset time. The patients' own difficulty to fall asleep, however, decreased significantly, and also a significant increase of dream recall could be observed.

Adult↗
PubMedDate not supplied

cAMP phosphodiesterase activity in diabetic rats eyes in normoxic, hypoxic and hyperoxic conditions.

Phosphodiesterase activity was tested on homogenized eyes of pre-diabetic rats kept in normoxic, hypoxic and hyperoxic conditions, with the aim of correlating any difference in PDE activity with variations in atmospheric oxygen contents. The activity of the cAMP phosphodiesterase (cAMP-PDE) was tested. Phosphodiesterases seem to be particularly susceptible to variations in oxygen tension, suggesting an important role of cyclic nucleotides in adaptive processes of the cell. cAMP-PDE activity increases slightly in normoxic, hypoxic and hyperoxic conditions in pre-diabetic rats. The second messenger, cAMP, plays important roles in mediating the biological effects of a wide variety of first messengers. The intracellular levels of cyclic nucleotides depend upon rates of synthesis and degradation, of cyclases and phosphodiesterase (PDEs), respectively. Therefore, PDEs seem to play an important role in a wide variety of physiological processes.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMedDate not supplied

Cyclic Amp phosphodiesterase activity in normal and inflamed human dental pulp.

Cyclic AMP phosphodiesterase (cAMP PDE) seems to be important in pulp tissues. High levels of cAMP PDE have been demonstrated to be in dental pulp cells. In the present study cAMP PDE activity was analyzed in normal healthy human dental pulps, in reversible pulpitis and in irreversible pulpitis. Enzymatic cAMP PDE control values for normal healthy pulps were 12.14 +/- 3.74 nmols/mg of proteins. In reversible pulpitis the cAMP PDE activity increased almost 2.5 times. In irreversible pulpitis specimens the values increased 4.5 times compared with normal healthy pulps activity. The differences between the groups (control vs. reversible pulpitis and vs. irreversible pulpitis) were statistically significant. These results could point to a role of cAMP PDE in the initial pulp response after injury.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMedDate not supplied

cGMP phosphodiesterase activity in diabetic rats eyes in normoxic, hypoxic and hyperoxic conditions.

Phosphodiesterase activity was tested on homogenized eyes of pre-diabetic rats kept in normoxic, hypoxic and hyperoxic conditions, with the aim of correlating any difference in PDE activity with variations in atmospheric oxygen contents. The activity of the cGMP phosphodiesterase (cGMP-PDE) was tested. Phosphodiesterases seem to be particularly susceptible to variations in oxygen tension, suggesting an important role of cyclic nucleotides in cellular adaptive processes. cGMP-PDE activity increases lightly in normoxic, hypoxic and hyperoxic conditions in pre-diabetic rats. The second messenger, cGMP, plays important roles in mediating the biological effects of a wide variety of first messengers. In particular, cGMP in the eye is an important mediator for the mechanism of sight. The intracellular levels of cyclic nucleotides depend upon the rates of synthesis and degradation, actuated, respectively, by cyclases and phosphodiesterases (PDEs). Therefore, PDEs seem to play an important role in a wide variety of physiological processes.

3',5'-Cyclic-GMP Phosphodiesterases↗
PubMedDate not supplied

Cyclic GMP phosphodiesterase activity role in normal and inflamed human dental pulp.

Cyclic GMP phosphodiesterase (cGMP PDE) plays an important role in pulp tissues. High levels of cGMP PDE are found in dental pulp cells. In the present study cGMP PDE activity was analyzed in normal healthy human dental pulps, in reversible pulpitis and in irreversible pulpitis. Enzymatic cGMP PDE control values for normal healthy pulps were 4.74+/-0.32 nmol/mg of proteins. In reversible pulpitis the cGMP PDE activity increased almost 3 times. In irreversible pulpitis specimens the values increased 4.5 times compared with the normal healthy pulps activity. The differences between the groups (control vs. reversible pulpitis and vs. irreversible pulpitis) were statistically significant. These results point to a role of cGMP PDE in the initial pulp response after injury.

3',5'-Cyclic-GMP Phosphodiesterases↗
PubMedDate not supplied

cGMP phosphodiesterase activity evaluation in human carcinoma of salivary glands.

The aim of this study was to evaluate differences of cGMP-PDE activity in salivary glands, between a control group and different benign tumour groups and, where present, with malign tumour groups. Endogen cGMP was evaluated too. The enzymatic reaction used the method of Spoto et al., with minor variations. The samples were organized in six groups: A (Adenolymphoma and Warthins tumour); B (Pleomorphic Adenoma); C (Basaloid Adenoma); D (Myoepitelioma). The control group was represented by healthy patients. In A and B groups, we have analyzed malign pathologies (Adenocarcinoma and Parotid Lymphoma) The benign tumours have more activity than controls, especially in Myoepitelioma (D) but with a decrement in the C group, which presents lower values of cGMP than the other three groups, where the concentration is similar. Between A and B groups, the activity values of malign tumours are similar, higher than controls and than the other benign pathologies, but not higher than in myoepitelioma. The cyclic concentration is similar for malign pathologies, with concentrations lower than controls, similar to Basaloid Adenoma (C).

3',5'-Cyclic-GMP Phosphodiesterases↗
PubMedDate not supplied

Glycoprotein distribution in non-histone chromatin proteins from pig liver.

Carbohydrate content of non-histone proteins from pig liver chromatin has been measured in different groups of chromatin fractions and does not seem to be related to the affinity of the proteins for DNA. Glycoproteins are preferentially located in the nuclease-sensitive fractions of chromatin. A 59,000 dalton glycoprotein has been identified as a characteristic components of a chromatin fraction solubilized by DNAase II.

Animals↗