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Biomedical subjects

G Speranza

Publications and source records attributed to G Speranza.

26 records · Page 2Linked to original sources
PubMed1985

Biliverdin as an electron transfer catalyst for superoxide ion in aqueous medium.

Stopped flow experiments gave evidence of the formation of a biliverdin-superoxide complex and/or a biliverdin radical anion by reaction of aqueous O2- with biliverdin. Such transient species are likely intermediates both in the bleaching of biliverdin, during exposure to the aerobic xanthine oxidase reaction, and in the reduction of ferricytochrome c under the same conditions.

Bilirubin↗
PubMed1983

The binding of bilirubin to albumin. A study using spin-labelled bilirubin.

Binding between human serum albumin and a spin-labelled derivative of bilirubin was investigated by circular dichroism, fluorescence quenching, electron spin resonance and visible spectroscopy. The orders of magnitude of the binding constants obtained by flurorescence quenching and electron spin resonance spectroscopies were 10(7) and 10(3) 1 . mol-1, respectively. These data suggest that most spin-labelled bilirubin interacts with human serum albumin at the side not holding the spin-labelled side-arm. CD measurements showed the presence of at least two sites, associated with opposite Cotton effects. It is worthy of note that the Cotton sign of the first site is inverted with respect to the corresponding one of bilirubin. CD measurements on mixed systems (spin-labelled bilirubin/human serum albumin/bilirubin) were also performed. The decomposition of the ternary curves shows that the rotatory power of bilirubin bound to human serum albumin is higher in the ternary system than in the binary (bilirubin/human serum albumin). The corresponding CD measurements for the binding between spin-labelled bilirubin and bovine serum albumin are also reported and discussed.

Animals↗
PubMedDate not supplied

[Exposure to low doses of CS2 and cardiovascular risk factors].

The effects were studied on cardiovascular risk factors of occupational exposure to carbon disulfide (CS2) concentrations ranging from 0.05 to 1.60 mg/m3, 74 workers exposed (E) to CS2 and 79 non-exposed (NE) workers were examined. Information was obtained on age, working age and life habits (alcohol, smoke, etc.) through a questionnaire and height and weight were measured to assess body mass index (BMI). All the workers underwent blood collection for the assessment of total cholesterol, HDL cholesterol and triglyceride levels and electrocardiogram. No clinically evident heart disease were detected in the workers examined and the prevalences of obesity and hypertension resulted to be similar among workers of the two groups. Mean triglyceride levels and frequencies of hypertriglyceridemia and of HDL cholesterol values < 40 mg/dl were significantly higher in exposed workers than non-exposed. The triglyceride levels resulted to be significantly and directly influenced by BMI and exposure to CS2. Occupational exposure to low environmental CS2 concentrations seems to interacts with other factors to determine alterations of lipid metabolism.

Carbon Disulfide↗