Biomedical subjects
Publications and source records attributed to G Smolin.
Locally administered antilymphocyte serum was irritating to the eye and failed to alter the production of antibody-forming cells in the homolateral lymph nodes, corneas, and uveal tracts of rabbits that had received intracorneal injections of bovine gamma globulin.
After the intracorneal injection of rabbit eyes with bovine gamma globulin, a systemically administered corticosteroid resulted in the following changes: (1) a reduction in the number of antibody-forming cells in the homolateral uveal tract and cornea; (2) a reduction in the ocular inflammatory response; and (3) a reduction in the number of antibody-forming cells in the draining lymph nodes, if high doses of corticosteroid were given.
Vaccinial epithelial keratitis was produced in rabbits. When the therapeutic effect of vidarabine on the experimentally-induced disease was evaluated and compared to the effect of idoxuridine, vidarabine was found to be highly effective, substantially more effective than idoxuridine, and nontoxic to the eye.
To study the effect of treating rabbits with a locally administered noncorticosteroid anti-inflammatory drug, we gave 26 rabbits unilateral subconjunctival injections of 15 mg of indomethacin suspension daily for 16 days, starting one day before the intracorneal injection of the same eye with bovine gamma globulin. We graded the eyes clinically and killed groups of rabbits on postimmunization days 6, 9, 12, and 15. We determined the number of antibody-forming cells in the homolateral cervical lymph nodes, corneas, and uveal tracts and tested the sera, aqueous humor, and vitreous humor for antibodies. Indomethacin-treated eyes showed significantly less inflammatory response, and an insignificantly greater number of antibody-forming cells, than the controls.
Of 34 species of Proteus isolated from human eyes, 29 (85%) were P. mirabilis and five (15%) were P. morganii. In vitro antibiotic sensitivity studies showed that gentamicin best controlled both P. mirabilis and P. morganii of all the antibiotics tested. In vivo tests on experimental Proteus infections of rabbit coreas, treated with gentamicin and tobramycin, yielded comparable clinical results, but gentamicin was more effective in eliminating the organism from the experimental lesions.
The local and systemic administration of human transfer factor to guinea pigs with herpetic keratitis or Candida albicans keratitis failed to alter the clinical or microbial course of the animals' disease.
When the ocular toxicity and the in vivo and in vitro effects of gentamicin, Nebcin, and saline solution were compared in experimentally induced Pseudomonas keratitis in rabbits, both antibiotics showed the same toxicity for the rabbits' conjunctival tissues. But Nebcin showed better in vitro and in vivo results than gentamicin, and the clinical effect was confirmed by culture study: significant numbers of organisms were recovered from the corneas of the gentamicin-treated rabbits but none from the corneas of the Nebcin-treated rabbits.
Significant numbers of antibody-forming cells (AFC) have been found in the cornea, uveal tract, and draining lymph nodes after the intracorneal injection of bovine gamma-globulin (BGG). To study the effect of locally administered corticosteroids on these antibody-forming tissues, we made unilateral intracorneal injections of rabbit eyes with BGG. These we followed immediately with subconjunctival injections of 10 mg. of triamcinolone suspension, and then with a second round of 10 mg. injections seven days later. A control group of animals received the BGG injections followed by two subconjunctival saline injections. We killed the animals on postinjection days 6, 9, 12, 15, and 21, and tested the draining lymph nodes, homolateral uveal tissue, and homolateral cornea for AFC by a modification of the Jerne placque technique. The local steroids had no effect on the number of AFC produced in the draining lymph nodes or on the circulating antibody response, but they reduced the number of AFC in the homolateral uveal tracts and corneas. Clinically there was less inflammatory response in the steroid-treated eyes than in the control eyes. The possible mechanisms by which corticosteroids achieve their anti-immunologic and anti-inflammatory benefits are discussed.
In vitro studies by workers in basic fields of biology have shown that halogenated DNA-base analogs are capable of sensitizing the DNA of various infectious agents in such a way that exposure to fluorescent light results in lethal damage to the DNA. In the present studies we treated experimental herpetic keratitis in rabbits with fluorescent light after using topical iododeoxyuridine (IDU) as a photosensitizing agent. So far as it clinical course was concerned, there was no significant difference between the disease treated with both IDU and light, and the disease treated with IDU alone. This was true in both the standard experimental disease and in the disease made more severe by prelimiary subconjunctival injection of corticosteroid.
Mycobacterium bovis (BCG, Pasteur strain) was injected intraperitoneally into guinea pigs and rabbits on the 19th and 5th days prior to the intracorneal inoculation of Herpesvirus hominis. During a three-day and nine-day observation period, respectively, the BCG had no significant effect on the clinical course of the herpetic keratitis and no effect on the titer of virus present in the infected corneas.
To study the effect of pretreatment of rabbits with locally administered steroids, unilateral subconjunctival injections of 10 mg. triamcinolone suspension were given 10 and 3 days before intracorneal injection of the same eye with bovine gamma globulin. We killed the rabbits 6, 9, 12, 15, and 21 days after the BGG injection, and determined the number of antibody-producing cells in the homolateral lymph nodes, corneas, and uveal tracts. Corticosteroids did not significantly affect the PFC response in the draining lymph nodes, but the number of plaque-forming cells in the corneas and uveal tracts was reduced. Steroid-treated eyes were less inflammed than control eyes. The possible mechanisms for the effect of steroids on the ocular immune response are discussed.
Several immunologic tests were used to compare the soluble antigens of cultured and normal bovine corneas. Results of fluorescent-antibody tests on normal corneas confirmed our earlier observation that the epithelium contained little serum protein, and that stroma and epithelium shared an antigen not found in bovine serum. Immunoelectrophoresis, immunodiffusion, and fluorescent-antibody tests preformed on extracts prepared from normal and cultured corneas indicated that corneas absorbed serum proteins during the incubation period. We also noted that very little intact epithelium remained on corneas cultured in the presence of calf serum. The extract prepared from cultured corneas did not contain the strongly antigenic protein present in extracts of normal cornea or epithelium. The implications of these findings are discussed.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.