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Biomedical subjects

G Sierra

Publications and source records attributed to G Sierra.

At least 55 records · Page 3Linked to original sources

[Use of boned fish meat in the preparation of dried products].

Minced fish flesh, both from Catfish (Arius sp) and from a mixture of various fish species of shrimp by-catch, were mixed with several starchy ingredients and salt. Drying at 70 degrees C was done until 4-10% moisture levels were reached. Physiochemical and microbiological tests (moisture, ash, protein, fat, pH, total volatile nitrogen, thiobarbituric tests, sodium chloride, color, total pour plate counts and yeast and mould counts) were conducted before and after drying. In addition, rehydration and sensory evaluation tests were performed in the dried products. Findings revealed that sodium chloride at 1.5% works as preservative and does not affect rehydration. Starchy ingredients favor fish particles binding and enhance sensory attributes such as color and appearance. Nevertheless, more studies in this area are recommended to reach acceptable quality products.

Animals↗

[A case of juxta-cortical chondroma (author's transl)].

A further case of juxta-cortical chondroma in the first finger-ray of a 61-year-old woman. Periosteal chondroma is an extremely rare benign tumor. Only 34 cases can be found in the literature. The tumor is cured with surgical removal.

Bone Neoplasms↗

Ultrasonic synergistic effects in liquid-phase chemical sterilization.

New methods of sterilization employing a chemical with moderate heat and ultrasonic energy have been devised. Inactivation of high-density bacterial spore suspensions is achieved by treatment with low concentration aqueous acid glutaraldehyde solutions at temperatures above or about 54 C. Low (20 kHz) or high (250 kHz) frequency ultrasonic energy is synergistic with glutaraldehyde. Rapid inactivation may also be achieved by using ultrasonic energy and aqueous alkalinized glutaraldehyde solutions at low (25 C) or moderate (55 C) temperatures. If compared to present room temperature techniques, "surface sterilization" time for contaminated objects can be reduced from hours to minutes.

Aldehydes↗

Mechanism and factors influencing the veronal inhibition of bacterial spore germination.

The inhibitory effect of sodium 5,5-diethyl barbiturate (Veronal) on the L-alanine-induced initiation of germination of Bacillus subtilis spores was examined. Veronal reversibly inhibited the initiation of germination by a noncompetitive mechanism. The inhibition was time-independent and it took place whether L-alanine was or was not allowed to permeate the spore before the addition of the inhibitor. The concentration of the inhibitor and the pH of the initiation system were important factors determining the effectiveness of Veronal as an inhibitor. The magnitude of the inhibition increased linearly with decreasing pH at constant concentration and with increasing concentration at constant pH. These results suggest that the inhibition involves a permeability phenomenon related to the access of drug to the active sites in the spore and that the entry of Veronal into the spores is regulated by the concentration of undissociated molecule. At the physiologically important pH of 7.4, initiation with alanine in phosphate buffer at high spore densities (about 10(9) spores per ml) was 50% inhibited by 4 mM Veronal, and 8mM Veronal inhibited initiation completely. L-Alanine initiation in tris(hydroxymethyl)amino-methane-hydrochloride buffer was completely inhibited by 5 mM Veronal. The inhibition could be partially reversed by the combined addition of D-fructose, D-glucose, and K(+). Possible reasons for the failure of otherwise inhibitory concentrations of Veronal to inhibit completely the L-alanine-induced initiation when a combination of fructose, glucose, and K(+) was present and a suggested relationship to two functional roles of L-alanine in the initiation of germination are discussed.

Alanine↗