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Biomedical subjects

G Siebert

Publications and source records attributed to G Siebert.

At least 55 records · Page 3Linked to original sources
PubMed1983

A note on the ADP-ribose-protein linkages in rat-liver nuclei: a possible approach to assessing megavitamin therapy with niacin.

Nuclei isolated from rat liver were incubated with NAD whose two ribose moieties were respectively labeled with 3H or 14C. By enzymatic (phosphodiesterase) and/or chemical (hydroxylamine) attack on doubly labeled ADP-ribosylated nuclear residues, AMP was found after hydroxylaminolysis as well as iso-ADP-ribose after phosphodiesterase plus hydroxylamine, in the absence of detectable amounts of ribose-5-phosphate. This is taken to indicate the existence of additional ribose-protein binding sites in in vitro ADP-ribosylated nuclear proteins: Besides C-1" (Hayaishi et al., Stocken et al.) C-2' and/or C-3' (purine-near) as well as C-2" and/or C-3" (pyrimidine-near), not only at the end but also within the chain of oligo-ADPR.

Adenosine Diphosphate Ribose↗
PubMed1982

Enzyme activity in human mononuclear blood cells.

Two different kinds of mononuclear blood cell samples (mixed blood cell population consisting of lymphocytes, monocytes, and platelets; pure lymphocyte population) were tested for enzyme activity of mitochondrial malic enzyme, peptidase A, and alpha-fucosidase. None of the three enzymes was demonstrable in the pure lymphocyte preparation; the mixed cell population, however, exhibited a distinct enzyme activity. Positive reactivity is thus obviously dependent upon monocytes and/or platelets.

Blood Platelets↗
PubMed1982

Biochemical characterization of the layers of subcutaneous adipose tissue in the pig body.

During the formation of two layers of adipose tissue in the pig's body, starting from the 80th day after birth, samples were obtained by biopsy and analyzed for gross constituents and enzymes concerned with fatty-acid biosynthesis. These two layers differ in total lipid and water content and demonstrate more subtle differences amongst DNA, protein, collagen and sodium concentrations when comparisons are made in regard to age, sex, and breeding selection for low-fat animals. Acetyl-CoA carboxylase, malic enzyme and glucose-6-phosphate dehydrogenase are more active in the inner layer, while 6-phosphogluconate and isocitrate dehydrogenases are distinguishable in the two layers of adipose tissue as well if age, sex, and breeding line are taken into consideration. The data form the basis for a more detailed study of lipogenic potentials in adipose tissue (next paper).

Acetyl-CoA Carboxylase↗