Biomedical subjects

G Sezille

Publications and source records attributed to G Sezille.

At least 55 records · Page 3Linked to original sources

PubMed1975

[Automation of fluorometric enzymatic determination of free and total cholesterol. Application to the determination of the esterification rate].

A fluorimetric, authomatical method for determination of free and total cholesterol in serum is described. Cholesterol esters are splitted into free cholesterol and fatty acids by cholesterol esterase. In the presence of oxygen free cholesterol will be transformed by cholesterol oxydase in delta 4 cholestenone with formation of hydrogen peroxyde. Hydrogen peroxyde oxidizes in the presence of catalase inethanol to formaldehyde which gives with ammonium ions and acetylacetone 3-5 diacetyl 1-4 dihydrolutidine measured by fluorimetry. Withour cholesterol esterase, the reaction allows a measurement of free cholesterol in plasma. So the ratio of ester to total cholesterol is determined by the two steps. Accuracy and precision are very good. Results are well correlated with those obtained with reference methods.

Autoanalysis↗

PubMed1975

[Metabolic study of 3 lipid emulsions used parenteral feedings. I. Exploration in vivo in the minipig].

The metabolism of three fat emulsions (Lipiphysan, Trive 1000, Intralipid) was compared when administered by intravenous route as complete parenteral nutrition in minipigs during 14 days. With Lipiphysan, an important accumulation of glycerides appeared in liver, spleen and lung whereas the phospholipids content of those tissues was unchanged. The three fat emulsions determined an increase of linoleic acid (18:2) percentages in glycerides and phospholipids of several tissues: liver, heart, lung, spleen and kidney. Moreover, at the level of phosphatidyl-ethanolamine (PE) in liver tissue, the three fat emulsions induced a decrease of some polyunsaturated fatty acids (C20 and C22) percentages. The observed differences in the amount of liver triglycerides between the three fat emulsions seem to be related with the removal mechanisms which appear to be dependent on each fat emulsion. In fact, all tissue lipid analyses showed an increase of 18:2 percentages which agree with an uptake of the different fat emulsions used. The decrease of polyunsaturated fatty acids percentages in PE might be due to an inhibition of elongationdesaturation system or a degradation of those fatty acids.

Animals↗

PubMed1975

[Metabolic study of 3 fat emulsions for intravenous nutrition. II. Study by isolated rat liver perfusion].

With a model of isolated rat liver perfusion, the authors study the removal kinetic of three different fat emulsions. In experiments done with Lipiphysan, triglyceride uptake by liver is linear with a rate of 30 mg/h until the 60th minute; after blockade of the reticuloendothelial system (RES), triglyceride uptake is greatly reduced. In standard experiments done with Intralipid and Trive 1000, triglyceride content of the perfusate does not vary significantly; however, the study of free fatty acids removal done before and after liberation of hepatic triglyceride lipase by heparin seems to show that Intralipid triglycerides are slightly hydrolyzed by hepatic triglyceride lipase. The particles of Lipiphysan might be capted directly by RES cells (fast removal), whereas Intralipid might be slightly hydrolyzed (slow removal); Trive 1000 removal appears to be slow and similar to Intralipid. Those results give some explanations on data obtained in vivo in minipigs and agree with a better use of Intralipid and Trive 1000 triglycerides by extrahepatic sites.

Animals↗

PubMed1974