Biomedical subjects

G Servillo

Publications and source records attributed to G Servillo.

39 records · Page 3Linked to original sources

PubMed1991

Variation of tyrosine aminotransferase expression during the day in rats of different ages.

The activity of the enzyme tyrosine aminotransferase and the synthesis of its specific mRNA were evaluated at different hours of the day in the liver of 3-, 12- and 24-month old BN rats. The enzyme activity has a circadian rhythm with a peak at midnight in 3- and 12-month old, which shifts to 03.00 hrs in 24-month old animals, in agreement with previous results. The expression of TATmRNA also changes during the day indicating circadian fluctuations which change with age. In 3-month old rats the TATmRNA peak is at 19.00 hrs, preceding that of the enzyme activity. In 12-month old rats the TATmRNA synthesis reaches a maximum at midnight and in 24-month old rats at 03.00 hrs. The results show that the circadian rhythm of tyrosine aminotransferase activity is due to a different gene expression throughout the day, which is influenced by age.

Aging↗

PubMedDate not supplied

The effects of hydroxyethyl starch solution in critically ill patients.

AIM: In the treatment of the critically ill patients an adequate fluid therapy appears to be essential to optimize hemodynamics and to get a suitable tissue perfusion. In this study we have evaluated the effects of volume replacement, carried out with 2 different solutions: hydroxyethyl starch 6% (HAES) and albumin 20% (HA). METHODS: Twenty patients suffering from sepsis were recruited and randomized into 2 groups. The first group was treated with hydroxyethyl starch 6% ( HAES treated group), and the second with albumin 20% (HA treated group). The volume of colloids was given to maintain pulmonary capillary wedge pressure (PCWP) between 15 and 18 mmHg. Daily, both hemodynamic parameters and blood gas analyses were monitored. RESULTS: Groups were homogeneous for age, sex and pathology. During the treatment we observed that cardiac index (CI), right ventricular ejection fraction (RVEF), oxygen consumption index (VO(2)I), oxygen delivery index (DO(2)I), and rate between arterial oxygen pressure and fraction of inspired oxygen (PaO(2)/FiO(2)) were increased significantly only in HAES treated group (P<0.05). APACHE II score decreased significantly only in HAES treated group (P<0.05), contrarily to the HA treated group, in which we observed a non significant increase. CONCLUSIONS: Since hydroxyethyl starch induced a hemodynamic and clinical improvement, these effects translated into an improvement of sensorium and a reduction of APACHE II score, without causing pulmonary edema, we can conclude that hydroxyethyl starch 6% ws 130,000 dalton ms 0.4 (Voluven) is an effective fluid for resuscitation of hypovolemic patients and represent an attractive alternative to albumin.

Adult↗

PubMedDate not supplied

[Monitoring cardiac output: esophageal doppler vs thermodilution].

OBJECTIVE: Evaluation of continuous cardiac output monitoring based on the esophageal Doppler in the critically ill. DESIGN: Prospective clinical investigation. SETTING: An intensive care unit of an University hospital. PATIENTS: Ten critically ill patients who needed pulmonary artery catheterization. MEASUREMENTS: Cardiac output was monitored continuously by a transesophageal Doppler device, consisting of an esophageal probe and a bedside microprocessor that calculated cardiac output using a new algorithm. Standard bolus thermodilution technique (10 ml of saline solution) was used to compare the continuous Doppler cardiac output measurement with the intermittent bolus measurement. MAIN RESULTS: A total of 50 pairs of intermittent (bolus) cardiac output and continuous (Doppler) cardiac output measurements were obtained from the 10 patients. The mean value of CO measured with TD was 5.81 +/- 0.83, while using esophageal Doppler was 5.84 +/- 0.81. The correlation coefficient of the two methods was r = 0.93. The Bland and Altman showed 95% of agreement limits as +0.52 and -0.64 L/min. CONCLUSIONS: Continuous monitoring of cardiac output using esophageal Doppler has proven to be safe, accurate, and precise when compared with the standard intermittent bolus thermodilution technique. The continuous monitoring technique improves our armamentarium for more intensive monitoring of the critically ill patients.

Adult↗