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G Seltmann

Publications and source records attributed to G Seltmann.

At least 55 records · Page 3Linked to original sources
PubMed1977

[Effect of peptidoglycan preparations on phage inactivation: decrease in phage 187 binding using monosaccharides].

Monosaccharides were found to be capable to decreasing the binding of phage 187 with peptidoglycan. Galactosamine hydrochloride and glucosamine hydrochloride proved to be the most effective, followed in order of decreasing activity by N-acetylgalactosamine, N-acetylglucosamine, glucose and galactose; mannose produced a weak effect. Ca2+ ions increased the reduction of phage peptiglycan binding with N-acetylgalactosamine and N-acetylglucosamine. Both of these monosaccharides also reduced the adsorption of phage 187 by the Staphylococcus aureus cells. In the medium enriched with Ca2+ ions; in the absence of Ca2+, they were, however, incapable of releasing the phage irreversibly bound with the peptidoglycan.

Acetylgalactosamine↗
PubMed1977

[Study of the chemical and immunogenic properties of the capsular Staphylococcus aureus antigen. 2. Evaluation of the immunogenic properties of the capsular antigen in experiments in vitro].

Albino mice were immunized with a purified capsular antigen isolated from the S. aureus strain 1193/74. The presence of specific anticapsular antibodies in the sera of animals were determined by two methods: 1) by conversion of diffuse growth of a homologous strain into compact one, and 2) by determination of opsonic index in phagocytosis of homologous staphylococci by human neutrophils. It was revealed that antibodies converting the microbical growth were absent in the sera of normal mice and reached the highest level after the second antigen injection; opsonins were present in the sera of normal mice in widely varying quantities; their maximal level was noted after the 3rd immunization.

Animals↗
PubMed1977

[Chemical and immunogenic properties of the capsular antigen of Staphylococcus aureus. I. purification and chemical characteristics of the capsular antigen].

Crude capsular material isolated from S. aureus strain 1193/74 was characterized chemically and subjected to purification by 4 different methods. The best results were obtained by the method including deproteinization of the material with a mixture of chloroform and butanol, fractional precipitation with ethanol and separation on SE-Sephadex. Galactose, uronic acids, and a small amount of protein served as components characteristic of the purified capsular material. Galacturonic and glucuronic acids were identified amoung the uronic acids. Besides, there were revealed other acids with a carbohydrate base, whose nature is still obscure.

Antigens, Bacterial↗
PubMed1976

[Chemical composition of the capsule material of Staphylococcus aureus strain 1193/74].

The capsule material of Staphylococcus aureus strain 1193/74 could be separated by precipitation with trichloroacetic acid and ethanol as well as by chromatography on DEAE-cellulose into 13 fractions. All fractions contained saccharides and uronic acids as well as amino acids and appeared in their qualitative composition rather similar. However, in quantitative composition and in chromatographic behaviour a rather high degree of heterogeneity could be observed. No clear cut separation of protein and polysaccharide material could be achieved in any fraction. It is supposed, therefore, that the capsule material does not represent merely an acidic polysaccharide, but contains certain amounts of amino acids or peptides varying in a rather wide range.

Amino Acids↗
PubMed1976

[The anodically moving thermolabile antigen (ATA) of gram-negative bacteria: its molecular heterogeneity and its chemical composition].

The anodically-moving thermolabile antigen (ATA) of gram-negative bacteria represents a glycolipoprote in. In aqueous solution ATA exhibits heterogeneity of the molecular size, the range varying between 2.10(4) and 8.10(5) Daltons. In the molecular range between 5.10(4) and 8.10(5) all the molecular states give precipitation lines with the corresponding antisera. It could be shown that all the molecular states of ATA are formed by different aggregation of the same subunit with a molecular weight of approximatively 22000.

Amino Acids↗
PubMed1975

[The anodic-moving thermolabile antigen (ATA) of gram-negative bacteria: its occurrence in rough mutants of shigella flexneri and Escherichia coli(author's transl)].

The thermolabile antigen (ATA) moving to the anode, discovered by us (9) and also described by LARSSON et al. (8), could be detected in Ra-, Rb-, Re- and Rd-mutants of Sh. flexneri and, in smaller amounts, in Rd- and Re- mutants of E. coli O8:K27:H. So it proves to be different from KUNIN'S common antigen (7). Our results lead to the conclusion that ATA is located in a more inner part of the bacterial cell wall and is fixed by noncovalent linkages.

Antigens, Bacterial↗
PubMed1975

[Dependence of various antigen formation of Proteus mirabilis strain 1095/67 growing on minimal nutrient media].

Proteus mirabilis strain 1095/67 has been cultivated in surface culture on different minimal media, which were composed either of single amino acids or combinations of several amino acids without or with stepwise addition of other C-sources up to the complete medium. We found a direct dependence of the formation of the various antigens (moving to the cathode, to the anode, or not moving) on the composition of the medium. Certain amino acids (Ala, Glu, Asp. Ser) elicit only parts of the antigenic spectrum formed in complete medium, but enrich special antigens. With only 2 amino acids (Ala and Glu) and the vitamins thiamine, pyridoxal, and niacine in the anorganic basic medium the complete antigenic spectrum can be formed. We have shown that the composition of the cell wall varies with the composition of the culture medium. In shaked cultures we found serine to be essential for the production of the antigen moving to the cathode and the thermolabile antigene moving to the anode (ATA), niacine to be of fundamental importance in the biosynthesis of all antigens. Already under very limited supply of nutrients ATA, common to all gram-negative bacteria, is formed and, therefore, it seems to be a very basic cell wall component.

Amino Acids↗