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Biomedical subjects

G Schieferstein

Publications and source records attributed to G Schieferstein.

At least 19 recordsLinked to original sources
PubMed2000

Oxalate in human seminal plasma: possible significance and problems of oxalate determination.

OBJECTIVE: To determine the level of oxalate in seminal plasma and to correlate it with andrologic variables. DESIGN: Laboratory examination. SETTING: University-based department of andrology and department of clinical chemistry. PATIENT(S): One hundred male outpatients with infertility. INTERVENTION(S): Isolated seminal plasma was stored for 120 days at -20 degrees C. MAIN OUTCOME MEASURE(S): Levels of oxalic acid in seminal plasma. RESULT(S): Oxalate levels in seminal plasma depend on the degradation of ascorbic acid to oxalic acid during storage. There was a linear nonenzymatic increase of oxalate with storage time. After 120 days of storage, the values were 133 +/- 50 micromol/L (n = 100). Oxalic acid correlated significantly with the number of sperm head anomalies, the pH value, and the age of the donors and correlated negatively with the volume of ejaculate. CONCLUSION(S): Oxalic acid in seminal plasma may influence male fertility.

Adult↗
PubMed1996

Mercury in urine and ejaculate in husbands of barren couples.

Mercury concentrations in morning urine and ejaculate were detected in 80 husbands of women presenting for infertility treatment. Additionally, the number of their dental amalgam fillings was documented. A routine spermiogram was performed, from which a numerical "fertility index" was calculated. Urinary mercury concentrations were in the range of non-exposed populations, only minute Hg concentrations were determined in ejaculate, 75% of the semen sample concentrations were under the detection limit of 5 micrograms/l. In comparison, 7 proven fertile workers with occupational mercury exposure had elevated levels of mercury in their ejaculates (range 10-65 micrograms/l). No positive correlation could be established between subject mercury concentrations in urine or ejaculate and the quality of their semen, expressed as fertility index. Equally, no such correlation could be established between the fertility index and the number of their dental amalgam fillings. From these preliminary data no evidence can be derived for the alleged relation between the mercury burden from dental amalgam fillings and male fertility disorders.

Adult↗
PubMed1995

Chronic study of triprolidine for oncogenicity in mice.

Triprolidine hydrochloride was fed to groups of 60 B6C3F1 mice per sex at dietary levels of 0, 500, 2000, or 4000 ppm (as the free base) for up to 2 years. Up to 12 mice of each sex and dose group were terminated after 65 weeks for hematology and clinical chemistry. The control and high-dose groups were examined histologically. A complete histopathological examination was performed on the remaining 48 mice from each dose group when removed from study due to moribund condition, early death, or terminal euthanization at 105 weeks. Triprolidine did not significantly alter the survival of either sex. High-dose male and mid- and high-dose female body weights were significantly less than controls at the end of the study. Significant trends toward lower frequency with increasing dose were noted in females for fatty change in the liver and lymphomas (combination of lymphocytic, mixed, and histiocytic lymphomas). Similar negative trends in males were for lymphocytic cellular infiltration in multiple organs and lung alveolar/bronchiolar adenomas or the combination of alveolar/bronchiolar adenomas or carcinomas. Significant trends toward increased frequency with increasing dose were found in female mice for lymphocytic infiltration in multiple organs and cytoplasmic alterations of the acinar cells of the parotid gland. Similar positive trends were found in males for cytoplasmic alterations of the parotid gland and various hepatocellular changes (e.g., hypertrophy and altered foci). While there was a positive dose response trend for hepatocellular adenomas in males the combination of these and hepatocellular carcinomas eliminated the significant trend, and it was concluded that there was no evidence of a carcinogenic response to triprolidine in B6C3F1 mice.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1995

Triprolidine: 104-week feeding study in rats.

The antihistamine triprolidine hydrochloride, was fed at dietary concentrations of 0, 250, 1000, or 2000 ppm (as the free base) to groups of 60 Fischer 344 (F344) rats of each sex for up to 2 years to evaluate its potential carcinogenicity. Up to 12 per sex from each group were killed at 65 weeks, and hematology, clinical chemistry, and histopathology were evaluated. A complete histopathological evaluation was performed on all other animals; survivors were killed at 2 years. Survival was significantly extended in triprolidine-treated males and females, particularly at the high dose. At the close of the study high-dose males and females had gained significantly less body weight than controls. Among rats killed at 65 weeks females in the mid- and high-dose groups weighed significantly less than controls, but weights of control and dosed males were not significantly different. The incidences of numerous lesions tended to decrease with increasing triprolidine dose. In females, clitoral gland adenomas, thyroid c-cell hyperplasia and neoplasia, mammary gland hyperplasia and fibroadenomas, and uterine stromal polyps, and in males, anterior pituitary gland adenomas, preputial gland neoplasia, thyroid c-cell pancreatic islet neoplasia, mononuclear cell leukemia, and the combination of lymphocytic, histiocytic, and undifferentiated cell malignant lymphomas and mononuclear leukemia, all exhibited negative dose trends. Cytoplasmic alterations of the parotid gland and numerous liver lesions tended to be more frequent in treated than in control animals. Liver lesions that exhibited positive dose trends include chronic inflammation and centrilobular fatty change in both sexes, mixed cell foci, and the combination of mixed cell foci and eosinophilic foci in females, and in males, basophilic foci and eosinophilic foci. Triprolidine was not carcinogenic in F344 rats.

Animals↗
PubMed1992

[Toxic epidermal necrolysis (Lyell's syndrome) after rubella].

Rubella was confirmed clinically and serologically in a 14-year-old boy who had been admitted with mild general symptoms and an exanthematous rash presenting with medium size maculae. Within only three days the exanthema had faded. Five days later the boy again became ill with high fever (40 degrees C) and renewed erythema with at first irregular then rosette-like and widely confluent spots. On admission several nonspecific inflammatory signs were markedly elevated (erythrocyte sedimentation rate, C-reactive protein, alpha-globulin). There were mild abnormalities of left-ventricular repolarization at the electrocardiography. The skin changes were progressive and at first interpreted as erythema exudativum multiforme. Fever persisted, despite antibiotic treatment, and signs of epidermolysis appeared. After the diagnosis of non-staphylogenic Lyell's disease was made high-dosage prednisolone administration was begun (initially 3 mg/kg in 6 hours, then 2.5 mg/kg daily for 8 days). The patient's condition and the electrocardiographic findings returned to normal within 2 days. The epidermolysis was arrested within a few hours and the blisters dried out within 24 hours. There were no complications. Since no drugs were given before the second day of epidermolysis being noted, it is highly likely that rubella precipitated Lyell's disease.

Adolescent↗
PubMed1990

Monoclonal antibodies against antigens expressed by human sperm.

The production and characterization of 21 mouse monoclonal antibodies (TüS1-TüS21) with specificity predominantly for human spermatozoa antigens is described. Reactivity of cells from human ejaculates, peripheral blood and several organs was determined using the alkaline phosphatase-anti-alkaline phosphatase (APAAP)-technique as well as the indirect immunofluorescence test. 15 of the monoclonal antibodies reacted with various regions of human sperm and often also with their precursor cells in the testis. Cross-reactivity with animal spermatozoa was frequently observed.

Acrosome↗
PubMed1987

[Value of detecting pathogens in fertility disorders].

The present study was carried out on 1092 asymptomatic male patients suffering from unexplained infertility without any history or clinical signs of inflammatory diseases of the genital tract. Semen samples were cultured for mycoplasma. In 214 of the patients, we additionally performed bacteriological investigations on seminal fluid, smears from the glans penis or prepuce, and urine specimens. Semen analysis included the common ejaculate parameters. We found significantly elevated counts of mycoplasma in 13% of the semen samples examined; pathogenic isolation rates of bacteria were observed in 36%. Increased counts of various bacterial species associated with high numbers of mycoplasma seem to be one of the causative factors in male infertility. There was not observed any close relationship between the recovery of microorganism and poor semen quality, except for fructose and citrate. When follow-up investigations were carried out in untreated patients, the concentrations of mycoplasma and bacteria in the semen fluid revealed a spontaneous variability of considerable extent, indicating frequent contamination of the semen. Therefore, we recommend to perform a culture control test of the semen prior to specific antibiotic treatment.

Adult↗
PubMed1986

Absence of HLA class I and class II antigens as well as beta 2-microglobulin from normal and pathological human spermatozoa.

Human spermatozoa from 87 donors with normal or pathologic semen specimen were examined for the expression of HLA-class I and class II antigens as well as beta 2-microglobulin (beta 2m) using a panel of monoclonal antibodies in an indirect immunofluorescence test. The results make it very unlikely that HLA-class I and class II molecules as well as beta 2m are expressed on human sperm cells.

Antibodies, Monoclonal↗
PubMed1985

[Education of the dermatologist in medical mycology].

Principal considerations on graduate medical education in mycology revealed that it should be more effective. For this purpose, an intensive training program has been performed at our department. Here we report on our experiences with this conception as well as our conclusions drawn from it.

Curriculum↗
PubMed1984

Immotile cilia syndrome: reduced chemotaxis and reduced number of intramembranous particles in granulocytes.

The neutrophil granulocytes of four patients with immotile cilia syndrome were investigated by means of freeze-fracture technique. Whereas most granulocytic functions (adherence, phagocytosis, killing of micro-organisms, reduction of NBT, and chemoluminescence) were in the normal range, chemotaxis of the neutrophils was clearly reduced; their plasma membrane revealed a profound reduction in the density of intramembrane particles. An interrelationship between reduced particle density and defective chemotaxis in neutrophils is assumed, but not yet proven.

Adult↗
PubMed1982

[Alkali neutralization and alkali resistance in persons with healthy skin and in eczema patients].

Alkali resistance (AR) and alkali neutralisation (AN) time, according to Tronnier and Bussius, were measured at three month intervals on 77 people with healthy skin and compared with test results from 205 patients with occupational dermatitis for whom expert opinion had been given once or several times. It was established that AN and AR react independently of one another. No significant difference was noted in either of the groups with reference to sex, age, occupation and skin type, nor in the ill patients with reference to the duration of the dermatitis, reduction in earning capacity, sensitivity or state of illness at the time opinion was given. Both AN and AR changed usually in the course of occupational dermatitis, however not parallel to one another, nor in relation to the respective skin condition. An and AR results from the dermatitis patients are significantly worse than those from persons with healthy skin tested as comparison. 35% of the healthy persons had pathological AR. In spite of considerable variations in AN in individuals, a satisfactory reproduction of the test results was obtained both for the AN and AR in repeated investigations.

Adult↗