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Biomedical subjects

G Sato

Publications and source records attributed to G Sato.

At least 91 records · Page 5Linked to original sources
PubMed1982

Identification of citrate utilization transposon Tn3411 from a naturally occurring citrate utilization plasmid.

We have isolated a new transposon, Tn3411, encoding citrate-utilizing ability, from a naturally occurring citrate utilization (Cit) plasmid, pOH3001. Citrate transposon Tn3411 was transposed from pOH3001 to lambda b519 b515 cI857 S7 (abbreviated lambda bb) phage, and further from the resulting lambda bb:Tn3411 to a vector plasmid, pBR322, in recA-deficient strains. The Cit+ plasmids (pOH2 and pOH3) constructed by the integration of Tn3411 into pBR322 were examined by restriction endonuclease and heteroduplex analysis. The results obtained were as follows: (i) Tn3411 was 7.4 kilobases long and flanked by small inverted repeats, and it contained one more pair of inverted repeats at the opposite orientation in the internal region, thus making alternate repeats; and (ii) the Cit+ structure gene was located on the fragment (5.5 kilobases) between two SalI cleavage sites on Tn3411.

Bacteriophage lambda↗
PubMed1982

Comparison of DNA sequences required for the function of citrate utilization among different citrate utilization plasmids.

The relatedness of DNA sequences encoding citrate utilization was examined by hybridization with a cloned DNA fragment from a citrate utilization (Cit) plasmid, pOH30221, and DNA of other Cit plasmids. This revealed that there are at least two groups of Cit plasmids: the Inc W Cit plasmids, which show homology with the probe, and the Inc H1 plasmids, which do not.

Base Sequence↗
PubMed1982

[Pre- and intra-operative chemotherapy using FT-207 suppositories--with special reference to lung cancer].

This study was conducted to determine efficacy of FT-207 to prevent hematogenous peri-operative metastasis of lung cancer. A total of 16 patients consisting of 10 with squamous cell cancer, 6 with adenocarcinoma were evaluated in this study. The protocol of this study consisted of the pre-and peri-operative chemotherapy, giving 1000 mg of FT-207 suppository daily for 10 days. 5-FU plasma concentration in the systemic circulation during surgery was 0.01 mcg/ml. 5-FU tissue concentration of the normal lung was 0.07 mcg/g, and cancerous tissue (squamous cell ca 0.11 mcg/g, adenocarcinoma 0.12 mcg/g), and lymph nodes 0.15 mcg/g. It was found in this study that the pre-and peri-operative adjuvant chemotherapy with FT-207 was effective to prevent operation-induced hematogenous metastasis of lung cancer.

Adenocarcinoma↗
PubMed1981

Alterations in growth requirements of kidney epithelial cells in defined medium associated with malignant transformation.

The possibility has been investigated that 1) the supplements required for the growth of the Madin Darby Canine Kidney (MDCK) cell line in serum-free Medium K-1 are indeed requirements for the growth of normal kidney cells in vitro, and 2) that alterations in these growth requirements are associated with malignant transformation. Consistent with the hypothesis that MDCK cells resemble normal kidney cells in culture, primary cultures of baby mouse kidney epithelial cells grow in Medium K-1 and respond to the 5 components in the medium. The growth properties of Moloney sarcoma virus (MSV)-transformed MDCK cells in defined media have been examined. Unlike MDCK cells, MSV-transformed MDCK cells form tumors in adult nude mice. Although they still respond to the 5 factors in Medium K1, the optimal dosage for insulin is lower for the MSV transformants than for MDCK cells. The MSV transformants also have an additional requirement for growth in Medium K-1-fibronectin. Variants of MDCK cells have been isolated that have lost the PGE1 requirement for growth in defined medium. These variant cells have acquired 1) the ability to form tumors in adult nude mice and 2) an alteration affecting cAMP metabolism, in addition to PGE1 independence.

Animals↗
PubMed1981

Change of drug resistance patterns and genetic properties of R plasmids in Salmonella typhimurium of bovine origin isolated from 1970 to 1979 in northern Japan.

A total of 321 Salmonella typhimurium strains of bovine origin obtained in northern Japan during the period 1970-1979 were tested for drug resistance and detection of conjugative R plasmids. Three hundred and eighteen (99.1%) of these strains were resistant to one or more drugs. The isolation frequently of multiply drug-resistant strains tended to increase year by year. Two hundred and thirty-seven (74.5%) of these resistant strains carried conjugative R plasmids. A total of 308 R plasmids including 174 (56.5%) thermosensitive (ts) R plasmids were derived from the 237 drug-resistant strains, indicating that 71 (30.0%) strains have two different conjugative R plasmids in a single host cell. Of the 308 R plasmids examined for fertility inhibition (fi), 167 ts and 131 non-ts R plasmids were fi-. Of the 60 ts r plasmids examined for incompatibility, 50 were classified into H1 group and 10 into H2 group. Of the 52 non-ts R plasmids examined, 35 were classified into the I alpha-group and the remaining plasmids were untypable in our tests. Mercury resistance marker was found in about 20% of H1 R plasmids coding for multiresistance, and all of H2 R plasmids coded for resistance to tellurite. The clonal distribution of an S. typhimurium strain which carried an H1 R plasmid coding for resistance to six drugs and mercury was recognized in 1978 and 1979.

Animals↗
PubMed1981

Incompatibility of citrate utilization plasmids isolated from Escherichia coli.

The 57 conjugative Cit plasmids isolated from 72 citrate-utilizing (Cit+) Escherichia coli strains from various sources were classified into four groups on the basis of their genetic properties. Escherichia coli K13 strains carrying these Cit plasmids could utilize cis-aconitate or tricarballylate, in addition to citrate.

Citrates↗
PubMed1981

Secretion pattern of pro-opiomelanocortin-derived peptides by a pituitary adenoma from a patient with Cushing's disease.

Fragments of the pituitary adenoma of a patient with Cushing's disease were maintained in defined culture medium. Immunoreactive (IR) ACTH, IR alpha-MSH, IR beta-lipotropin (beta-LPH), IR beta-endorphin, and IR gamma-MSHs secreted from the adenoma were studied with gel permeation chromatography and the respective RIAs. The adenoma secreted roughly equimolar quantities of IR beta-LPH plus IR beta-endorphin, IR gamma 3-MSHs, and IR ACTHs. It also secreted IR alpha-MSH as well as IR gamma 1-MSH, although in a much lower concentration than the above four peptides. The secreted gamma 3-MSH-like peptides were found to be glycosylated. The secretion pattern suggests that this particular adenoma processes the pro-opiomelanocortin molecule in pathways which encompass those of both the pars distalis and the pars intermedia.

Adenoma↗
PubMed1981

Delineation of voluntary movement in Parkinson's disease with the manual control system.

An assessment of the dynamic characteristics of voluntary movement in 20 patients with Parkinson's disease and 20 normal subjects was performed by the manual control system. The following eight parameters were estimated and mapped on the plane with eight axes: gain constant between the input and the output, cut frequency for evaluation of the frequency response of human operator, dead time, square error, correlation coefficient, and three different points of coherency for the measurement of the linearity of human operation. In Parkinson's disease, we found a low gain constant of 8.34 +/- 9.64 dB (mean +/- S.D.) (26.5 +/- 2.12 in controls), a narrow cut frequency of 1.08 +/- 0.91 radians/sec (5.65 +2- 1.25), a long dead time of 0.79 +/- 0.27 sec (0.19 +/- 0.05), a large square error, a low correlation coefficient, and low points of coherency. Among the eight parameters, the gain constant and the dead time proved to be the most useful for quantitative assessment of motor disability for this disorder. Our method promises to be valuable to confirm the effects of drug therapy and the progression of the disease.

Adult↗
PubMed1981

Biotyping of Salmonella typhimurium strains isolated from animals and birds in northern Japan.

Strains of Salmonella typhimurium (n = 175) isolated from animals and birds in northern Japan were differentiated into 5 biovars (1, 2, 7, 10, and untypeable) by 6 kinds of fermentation tests (inositol, bitter-xylose, rhamnose, xylose, Stern's glycerol, and trehalose) of Brandis' scheme, and were subdivided into 9 primary and 38 full biovars by a new biotyping method, using additional biochemical reactions. The full biovar classified by the new biotyping method was considered to be a marker for assessing the widespread outbreaks of infection with S typhimurium. In particular, strains of biovars 25hi and 27hi were characteristically found in pigeons, quail, and fantails, and were thought to be of avian origin.

Animals↗
PubMed1981

Growth characteristics of primary tissue cultures from heterotransplanted human colorectal carcinomas in serum-free medium.

Procedures are described for the preparation of reproducible primary cultures from human colorectal tumors transplanted in the athymic nude mouse and for the quantitative evaluation of growth by means of counting suspensions of nuclei from these cultures with a Coulter counter. Growth curves of primary cultures from 11 colorectal tumors in serum-free medium are shown and discussed with respect to the in vitro conditions to be met for the propagation of in vivo stem cell populations.

Animals↗
PubMed1980

Growth of functional primary cultures of kidney epithelial cells in defined medium.

Primary cultures of baby mouse kidney epithelial cells can grow without fibroblast overgrowth in a hormone-supplemented serum-free medium (Medium K-1) designed for an established kidney epithelial cell line, MDCK. The five supplements in Medium K-1 are insulin, transferrin, PGE1, T3, and hydrocortisone. Medium K-1 also supports the growth of kidney epithelial cell cultures from a number of animals, including man, without fibroblast overgrowth. Outgrowth of kidney epithelial cells from kidney explants was also observed with Medium K-1. Thus, the medium appears to be selective for epithelial cell growth. The physiological properties of primary cultures of baby mouse kidney epithelial cells were studied in detail. Baby mouse kidney epithelial cells grew at equal rates (0.5 doublings/day) in Medium K-1 and serum-supplemented medium. Medium K-1 also supported the formation of baby mouse kidney epithelial colonies at low cell densities. The dependence of baby mouse kidney epithelial colony formation upon the five factors in Medium K-1 was examined. These studies indicated that the formation of baby mouse kidney epithelial colonies in defined medium depended upon all the five supplements in Medium K-1, in a manner similar, although not identical, to MDCK colonies. Primary cultures of baby mouse kidney epithelial cells grown in Medium K-1 retained kidney cell-associated properties, including the ability to form multicellular domes, a phenomenon associated with transepithelial salt transport. Amiloride-sensitive Na+ uptake and the mucosal surface enzyme leucine aminopeptidase were also observed in baby mouse kidney cultures. Similar functions were observed in MDCK monolayers.

Animals↗
PubMed1980

Effects of a serum spreading factor on growth and morphology of cells in serum-free medium.

A heat-sensitive, trypsin-sensitive factor that promoted growth and spreading of cells in serum-free, hormone-supplemented medium was partially purified from human serum. The major portion of the proteins in these preparations migrated upon SDS-polyacrylamide gel electrophoresis with a mobility consistent with molecular weights between 60,000 and 90,000. The spreading activity, which we have termed serum spreading factor, stimulated growth and spreading of a wide variety of cell types. The serum spreading factor was similar to fibronectin in that it showed an affinity for the plastic cell culture substrate but was shown to be distinct from fibronectin by several criteria. This factor may prove useful in studies of cell attachment and spreading and in studies of the relationship of cell shape and cell proliferation.

Animals↗