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Biomedical subjects

G S Komolova

Publications and source records attributed to G S Komolova.

At least 19 recordsLinked to original sources
PubMed2001

[Angiogenin content in human milk in the early period of lactation].

The content of angiogenin in human milk and dairy produce for feeding of children of the first year of life is investigated. It is revealed, that the highest concentration of angiogenin in human milk takes place in colostric period (18.00 +/- 0.72 mg/l). During the subsequent time of lactation the concentration of angiogenin keeps at a level 2.00 +/- 0.06 mg/l. In products of infant's feeding the angiogenin either was absent, or its concentration was much lower, than in breast milk.

Female↗
PubMed1997

[Determination of angiogenin in cow's milk based on a competitive test "pancreatic RNAase/placental inhibitor of RNAase--angiogenin"].

Contents of active factor of blood vessel growth angiogenin was analyzed in cow's milk by the method of competitive test in system "pancreatic RNAase/placental inhibitor of RNAase-angiogenin". High level of RNAase in milk limits using this test. To avoid this limitation the method of RNAase elimination from milk was elaborated.

Angiogenesis Inducing Agents↗
PubMed1995

[Quantitative determination of cattle angiogenin].

Polyclonal antibodies to a milk antigen were obtained by a standard immunization procedure. The possibility was demonstrated to use the peroxidase-antibody conjugate in a competitive immunoenzymatic test. The minimal amount of the antigen determined by this method is 10 ng/ml.

Angiogenesis Inducing Agents↗
PubMed1990

Expression of the hAng gene in Escherichia coli; isolation and characterization of human recombinant Ser-(-1) angiogenin.

Recombinant human angiogenin has been synthesized in Escherichia coli with the aid of a human angiogenin gene (hAng) cloned by Neznanov et al (1990) from a human complementary DNA (cDNA) library. The gene has been expressed by use of a new type of expression vector called a 'TGATG vector' (plasmid pPR-TGATG-1; Mashko et al 1990a). The highest level of accumulation of the recombinant angiogenin (6%-8% of the total cell protein) was observed in E. coli strain BL21 carrying a temperature-amplifiable version of the plasmid. The synthesized polypeptide carries an additional serine residue at its N terminus in comparison with natural angiogenin. Furthermore, the initiator methionine residue of the recombinant protein is removed with high efficiency by E. coli terminal aminopeptidase. Simple procedures for purification of the recombinant angiogenin from the insoluble fraction of cell protein, and for refolding the protein allowed the isolation of almost 5 mg recombinant angiogenin g-1 wet bacterial biomass. The recombinant Ser-(-1) angiogenin displayed the same biological properties (specific RNAase activity and the ability to induce blood vessel growth on the sclera of experimental animals) as its natural counterpart isolated from human blood.

Amino Acid Sequence↗
PubMed1989

[Inhibition of synthesis of nucleic acids, proteins and respiration in isolated thymocytes by anthracyclines].

The effect of anthracycline antibiotics such as carminomycin, daunomycin (rubomycin) and adriamycin on respiration and synthesis of nucleic acids and protein was studied comparatively. The anthracyclines inhibited the processes. By their efficacy in that respect they could be arranged in the following order: carminomycin greater than rubomycin greater than adriamycin. Thus, 50 per cent inhibition of nucleic acid synthesis in the thymocytes required 0.027, 0.044 and 0,173 mM of carminomycin, rubomycin and adriamycin respectively. Protein synthesis and respiration in the thymocytes were less sensitive to the effect of the anthracyclines than synthesis of nucleic acids. The study results were compared with the literature data on the effect of the compounds on respiration and synthesis of nucleic acids and protein in tumour and bacterial cells.

Animals↗
PubMed1989

[The effect of alkylresorcinol on the respiration and synthesis of nucleic acids and proteins in isolated thymocytes].

The effect of the membranotropic agent alkylresorcinol 5C10 on the respiration, nucleic acid and protein synthesis in isolated thymocytes was studied. Within the 5C10 concentration range of 10(-7)-10(-5) M, the inhibition of respiration and incorporation of labelled precursors into thymocyte proteins and DNA was observed. In case of respiration and protein synthesis, a 50% inhibition was observed at alkylresorcinol concentrations of 10(-8) and 0.5.10(-5) M, respectively. The rate of 3H-thymidine incorporation into DNA progressively decreased already at 5C10 concentration of 10(-7) M. At 10(-6) M alkylresorcinol its inhibiting effect on DNA synthesis was about 30% and it did not change with a further rise in the inhibitor concentration up to 10(-5) M. In contrast, the rate of RNA synthesis significantly increased (ca. by 20%) within the alkylresorcinol concentration range of 10(-6)-10(-5) M. At 5C10 concentrations above 10(-5) M, the state of thymocytes in the preagglutination period appeared to be critical and was characterized by a dramatic inhibition of all the parameters under study. The experimental results suggest that alkylresorcinol 5C10 causes the inhibition of processes that are functionally coupled with biological membranes.

Animals↗
PubMed1981

[DNA and RNA synthesis in isolated nuclei of rat skeletal muscle fibers following hypokinesia].

Incorporation of labelled nucleotides [3H] UTP and [3] TTP revealed the intensity of DNA and RNA synthesis in the skeletal muscle nuclei in vitro in the male rats of Wistar line after 22-day immobilization. The RNA biosynthesis (the intensity of transcription) considerably diminished in the skeletal muscle nuclei of hypokinetic rats (by 1.8 times). No differences developed in the actinomycin D depression of r-RNA and s-RNA synthesis. The DNA biosynthesis (the intensity of replication) also decreased. The depression of DNA and RNA biosynthesis seems to be the main reason of the decreased intensity of protein synthesis in the rat skeletal muscle during hypodynamia.

Animals↗
PubMed1980

[Effect of hypokinesia on nucleic acid and protein metabolism in the lymphoid organs of rats].

Metabolism of nucleic acids and protein by lymphoid cells of the rat spleen and thymus was studied under conditions of 22-day hypokinesia. It was shown that in the course of hypokinesia the loss of cellular mass by the spleen and thymus was associated with varied biochemical changes in the remaining lymphoid cells. The thymocytes showed a significant activation of nucleic acid and protein biosynthesis. Meanwhile in spleen lymphocytes, DNA and RNA metabolism was inhibited with no appreciable changes in protein metabolism. Potential mechanisms of changes in metabolism of thymus and spleen lymphocytes under long-term hypokinesia are discussed.

Adaptation, Physiological↗