Biomedical subjects
Publications and source records attributed to G S David.
When trypsinized cells were treated with alloantibody and complement they initially adhered to their substratum, then detached. Specific cytolysis did not occur. In addition to demonstrating an immunologically mediated reaction, this phenomenon has permitted the development of a new, simple, rapid assay for alloantibody that does not require a cytolytic reaction.
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Homozygous suppression of allotypic specificities on both the heavy and light chains of rabbit immunoglobulin has been achieved by two methods involving embryo transfer. In the first method, young were transplanted into does producing antibody against an allotypic specificity homozygously present in the genome of the embryo. In this case, the suppression is caused by antibody transmitted in utero. In the second method, young from a nonimmunized surrogate mother lacking their allotypic genotype are injected neonatally with the antiallotype serum. Release from homozygous suppression appears to be achieved more readily than from heterozygous suppression.
Because monoclonal antibodies can recognize and bind to specific groups of atoms such as tumour antigens, they have promise for use in vivo as carriers of radionuclides, drugs or other appended molecules for diagnosis and treatment of disease. Attachment of metal ions to antibodies by means of bifunctional chelating agents can add the diverse nuclear, physical and chemical properties of the metallic elements to these specific binding proteins (ref. 4 and refs therein). With the ultimate aim of engineering probe-binding properties into the antibodies themselves, we have now prepared monoclonal antibodies against the EDTA chelate of indium. These antibodies show a remarkable preference for indium chelates; changing to another metal such as scandium or gallium can decrease the antibody-binding constant by more than three orders of magnitude. These antibodies also introduce a new degree of control over the biological distributions of chelated radionuclides, markedly altering their uptake in tumours and normal organs.