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Biomedical subjects

G Rzesa

Publications and source records attributed to G Rzesa.

At least 37 records · Page 2Linked to original sources
PubMed1978

Mixed connective tissue disease (MCTD)--its immunological characteristics.

The concept of the MCDT is controversial; it is still not settled whether it is nosological entity, or a variety of SLE, or a special variety of scleroderma. From the practical point of view the authors consider it important to perform tests for RNA antibodies whenever the characteristics of different collagenoses coexist. Detection of the antibodies is relevant both to the prognosis and the choice of treatment.

Antibodies, Antinuclear↗
PubMed1978

Crithidium luciliae immunofluorescence test (CL test) a simple and reliable method for detecting anti-native DNA antibodies.

Antibodies directed against native DNA using Crithidium lucillae as an antigen substrate were demonstrated in 39 of 72 cases of systemic lupus erythematosus (SLE) with active lesions whereas results were negative in 42 cases of SLE during remission and in 69 cases of other collagenoses, and in 20 healthy subjects. A clearcut correlation with the peripheral type of fluorescence of anti-nuclear antibodies (ANA) was found in the routine method of immunofluorescence on the monkey esophagus. Renal lesions were present in almost all cases of SLE with positive results of the test for Crithidium luciliae. The new technique of detecting antibodies against native DNA is simple and creates new diagnostic and prognostic possibilities in SLE.

Antibodies, Antinuclear↗
PubMed1978

Autoimmune phenomena in psoriasis.

The striatum corneum (SC) antibodies are present in all human sera as seen by indirect immunofluorescent (IF) staining. They appear to bind in vivo to the stratum corneum of psoriatic lesions. They fix complement in vitro in a two step complement IF test system using either anti C4 or anti C3 conjugates as indicators. IF tests with proper controls showed that the SC antigen in psoriatic scales is coated not only with IgG but in a majority of the lesions also with complement. These observations afford indirect evidence for the participation of SC antibodies and the ensuing fixation of complement in the development of psoriatic lesions.

Autoantibodies↗
PubMed1978

Studies on the Koebner phenomenon.

The examination of induced Koebner reactions in 71 patients by the IF methods and by histology at selected times after scratching, show, that immunologically specific but non-disease specific reactions of stratum corneum (SC) antibodies occur adjacent to the crusts in both control subjects and psoriasis patients. In lesions induced by scratching in patients with psoriasis there develop progressively additional foci of apparent in viveo binding of SC antibodies. IF deposits may appear before histologic or clinical changes. The widespread immune deposits in the SC with typical histologic changes of psoriasis seem to be a sign of impending clinical psoriasis while single foci of in vivo bound SC antibodies may be cleared by the body without the development of psoriasis.

Autoantibodies↗
PubMed1977

Substrate specificity of antinuclear antibodies in scleroderma.

Studies of antinuclear antibodies (ANA) were carried out in 39 cases of systemic scleroderma and for comparison in 19 cases of systemic lupus erythematosus (SLE) and 4 of mixed connective tissue disease (MCTD) using indirect immunofluorescence (IF) methods under standard conditions. The results on three different substrates--monkey esophagus, guineapig lip and rat liver--are reported. In 48.7% of scleroderma cases ANA showed a substrate specificity. The highest percentage of positive results in scleroderma was obtained on monkey esophagus (97.4%) and the lowest on rat liver (61.5%). In SLE and MCTD, in contrast, only about 13% of the sera displayed such specificity. If only sera with substrate specificity are considered, the positive results on monkey esophagus and rat liver are 94.7% and 21.1%, respectively. Titers of sera reacting positively on 2 or 3 substrates were mostly in agreement, although some sera both in systemic scleroderma and SLE showed higher titers on monkey esophagus. The IF pattern was usually the same regardless of the substrate, Tests for ANA in scleroderma should be performed on at least 2 substrates simultaneously.

Adult↗
PubMed1976

Dermatitis herpetiformis and bullous pemphigoid. Intermediate and mixed forms.

Nine patients had clinical and histological features suggestive of both dermatitis herpetiformis (DH) and bullous pemphigold (BP). Five patients responded to treatment with sulfapyridine or sulfones: in two the response was inconsistent, and the disease was controlled by combined treatment with prednisone; in one patient, there was no response to sulfapyridine or sulfones. Immunofluorescence studies showed IgA deposits in a linear homogeneous pattern at the basement membrane zone in all patients, and IgG was present in five. No circulating anti-basement membrane antibodies were detected by repeated immunofluorescence examinations. The authors conclude that the occasional overlapping of BP and DH should not lead to dropping the distinction between the two entities. For overlap cases that cannot be classified as BP or DH, the term "intermediate or mixed form of DH and BP" seems to be most suitable.

Aged↗
PubMed1976

Immunological phenomena induced by UV rays.

Antiserum against UV-irradiated DNA was prepared and used as a specific reagent in an indirect immunofluorescence (IF) technique for the detection of photochemically damaged DNA in the epidermis of hairless mice. Fluorescence of cell nuclei was found in sections of dorsal epidermis of mice immediately after the irradiation. It persisted for 24 hours. The IF reaction became negative after 48 hours, irrespective of the duration of UV exposure to which the mice were subjected. This may indicate: either (a) the occurrence of DNA repair processes, or (b) the relation of DNA repair to the life cycle of the epidermal cell. Mice exposed to UV radiation similar to the erythema spectrum of sunlight do not show any changes in the cellular DNA, even after a dose of 40 MED.

Animals↗
PubMed1975

Immunologic phenomena in herpes gestationis.

Immunofluorescence findings are of diagnostic significance in herpes gestationis. The studies in two cases demonstrated some complement components (chiefly C3 and C4, but no C(1q)), without immunoglobulins at the basement membrane zone and with no circulating anti BMZ antibodies. In one case IgG were found at the BM zone in one biopsy specimen, but were not demonstrable in several other biopsies. The IF findings seem to indicate both the alternate and classical pathways of complement activation, and speak in favor of a relation between herpes gestationis and bullous pemphigoid, in which sometimes the immunoglobulins are also not demonstrable and the immunofluorescent pattern is very much like that of herpes gestationis.

Adult↗
PubMed1975

Diagnostic importance of immunofluorescence in oral bullous diseases and lupus erythematosus.

Immunofluorescent tests have proved to be of diagnostic importance for pemphigus, bullous pemphigoid, cicatricial pemphigoid, systemic lupus erythematosus, and discoid lupus erythematosus. Immunofluorescence test procedures, necessary specimens, and test findings have been reviewed as aids to dentists in the utilization and interpretation of these tests for the study of oral lesions.

Antibodies, Antinuclear↗
PubMed1975

Studies in immunodermatology. VI. IF studies of autoantibodies to the stratum corneum and of in vivo fixed IgG in stratum corneum of psoriatic lesions.

Indirect immunofluorescent (IF) tests on sections of normal human skin reveal the presence of antibodies to the stratum corneum in most normal human sera. Sera absorbed with hyperkeratotic scales gave negative reactions. These reactions appeared to correspond to those of the stratum corneum antibodies which were first detected by Krough and Tonder by immune adherence, though this remains to be documented since the conjugate alone stained the stratum granulosum in a pattern comparable to that of the IF staining reported by Krough. The staining was caused by conjugates with high but not by those with low fluorescein to protein ratios. In indirect IF tests, comparable titers of stratum corneum antibodies were found in sera of psoriasis patients and control subjects. Since they reacted with the stratum corneum of the antibody producer they are referred to as autoantibodies. Direct IF tests of psoriatic lesions revealed the presence of in vivo bound IgG as well as other immunoglobulins and complement in the stratum corneum. To differentiate staining with conjugated protein from direct and indirect IF staining of the stratum corneum a four-compartment test was devised. Skin sections were treated with saline or with stratum corneum antibodies and then with conjugates without (compartments 1 or 2) or with (compartments 3 or 4) anti-IgG antibodies. Sections were read by the method of Kawamura and his associates with both UV and BV illumination. Compartment 3 is a direct IF test and compartment 4 is an indirect IF test. In four-compartment tests performed on both biopsies of psoriatic lesions or scales the difference between compartments 1 or 2 and 3 affords a measure of in vivo bound IgG and the difference between compartments 3 and 4 provides information on the presence of free stratum corneum antigen. With this four-compartment test system it was found that in vivo fixation of IgG occurs in the intercellular areas of the stratum corneum of psoriatic lesions or scales and that this corresponds to the site of binding of the stratum corneum autoantibodies. This in vivo binding of IgG in the stratum corneum of psoriatic lesions resembles somewhat the intercellular fixation of IgG in the stratum spinosum in pemphigus. In the materials examined in these preliminary studies most, if not all of the stratum corneum antigen appeared to be covered with in vivo bound IgG.

Animals↗