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Biomedical subjects

G Ruhenstroth-Bauer

Publications and source records attributed to G Ruhenstroth-Bauer.

At least 55 records · Page 3Linked to original sources
PubMed1981

Sensitive three-parameter flow-cytometric detection of abnormal cells in human cervical cancers: a pilot study.

The cell volume, the DNA, and the carcino-embryonic (CEA) or epithelial-membrane (EMA) antigen of formaldehyde-fixed human cervical cells from 21 malignant cervix tumors and 11 normal patients were measured simultaneously with a Fluvo-Metricell flow cytometer. The simultaneous cell volume and DNA measurement provided the distinction of morphologically intact cells from cell debris, the determination of the cell cycle phase combined with the detection of aneuploid cells, and the distinct of inflammatory cells from parenchymal and tumor cells. Malignant samples were recognized because they contained more than 0.5% CEA positive cells which were of intermediate size. CEA and EMA expression in the malignant samples was not linked. The false positive rate in a total of 32 samples was 6.3% when the sum of CEA and EMA positive cells of each cell sample was calculated. No false negative malignant sample was observed.

Adult↗
PubMed1981

Demonstration of antiinflammatory activity of fibrinogen and fibrinopeptides in rats.

Carrageenin-induced inflammatory rat paw swelling was significantly inhibited by intraperitoneal injection of rat fibrinogen. Whole-body radioscanning of the rat after intraperitoneal administration of 131I-labeled fibrinogen revealed the accumulation of radiolabeled material in the inflammed rat paw. Resorption studies showed that not more than 4% of the intraperitoneally administered [125I] fibrinogen could be demonstrated in the peripheral blood. Furthermore only 1/3 of this circulating radiolabeled protein was able to take part in clot formation, suggesting that inhibition of edema formation is mediated by fibrinogen-derived split products. This is further supported by the finding that rat fibrinopeptides, released by the action of thrombin, also diminish edema formation both after intracardial and intraperitoneal injection into carrageenin-stimulated rats.

Animals↗
PubMed1981

Flow-cytometric measurements of the transmembrane potential, the surface charge density and the phagocytic activity of Guinea pig macrophages after incubation with lymphokines.

The effects of lymphokines on guinea pig peritoneal macrophages were measured via flow cytometry utilizing the three-parameter FLUVO-METRICELL flow cytometer. On the basis of cell volume three distinct macrophage populations could be distinguished. Three to 5 min after starting the incubation with lymphokines a hyperpolarization of all three macrophage populations took place which was followed by depolarization. After 60 min the transmembrane potential reached again its control values. The negative charge density of the cell membrane decreased shortly after beginning of the incubation to 70-80% of the initial value and then remained unchanged for the following 120 min. The phagocytic activity of the macrophages was diminished during the depolarisation phase but increased over control values after restoration of the transmembrane potential.

Animals↗
PubMed1981

Kinetics of the acute-phase reaction in rats after tumor transplantation.

Transplantation of Yoshida sarcoma (solid type) and Zajdela ascites hepatoma tumors in rats induces a biphasic change in the concentration of the following five acute-phase proteins: alpha-1-acid glycoprotein; alpha-1-antitrypsin; haptoglobin; hemopexin; and ceruloplasmin. These proteins and other plasma proteins were quantitated by two-dimensional immunoelectrophoresis relative to normal serum concentrations. The elevation of most of these acute-phase proteins was greater in the second phase, during which serum levels increased continuously as the tumor burden increased until the animals died. The increase in haptoglobin concentration during the second phase was much higher in rats bearing Yoshida sarcoma than in rats bearing Zajdela tumors. Rats receiving irradiated tumor cells showed neither tumor growth nor second-phase protein changes. Significant increases in uptake of 3H-amino acids by isolated perfused livers of tumor-bearing rats provided evidence for an increase in the hepatic synthesis rates of the acute-phase proteins. Removal of the solid tumor resulted in a gradual decrease of acute-phase protein concentrations with concomitant increase in serum albumin concentration. These alterations in serum acute-phase proteins during tumor growth and after removal of the tumor may make their use attractive as biological markers of the response of the tumor-bearing animal to its tumor.

Animals↗
PubMed1980

[Postnatal development of rat thrombocytes: the volume distribution and electrophoretic mobility or rat thrombocytes during the first month of life (author's transl)].

The volume distribution curves of rat thrombocytes show a significant rise of the modal volume between days 5 and 8 after birth. No clear evidence for two distinct thrombocyte populations was obtained by computer analysis of the volume distribution curves. However, the increase of the modal volume of the platelets correlates with the production of a new erythrocyte population. In addition, the platelet concentration in the blood increases significantly during this time. These changes could indicate the production of a second thrombocyte population. The mean electrophoretic mobility of rat thrombocytes decreases only slightly during the first 3 weeks after birth, while it increases for erythrocytes significantly during the same period.

Animals↗
PubMed1980

Evidence for and characterization of a liver cell proliferation factor from blood plasma of partially hepatectomized rats.

A boiled extract acidified to pH 5.5 from the blood plasma of partially hepatectomized rats was treated with neuraminidase and injected i.p. into untreated rats. The DNA-synthesis of the liver cells showed a four-fold increase in comparison with controls. Extracts from the plasma of partially hepatectomized rats without neuraminidase treatment showed no increase in DNA-synthesis. Injections of boiled acid extracts from plasma of normal rats, however, showed no comparable differences before and after neuraminidase treatment. The activity of neuraminidase treated boiled, plasma extract is lost after treatment both with trypsin-chymotrypsin and with beta-galactosidase. Gel chrmoatography of the factor gave a molecular weight of about 38,000 D. The specific activity of the active extract after chromatography was raised by a factor of 300. According to affinity chromatography the factor was shown to be a glycoprotein containing N-Ac-glucosamine. The factor is inert with respect to the proliferation of spleen and kidney, i.e. it is organ specific. According to these results a regulatory system of hepatopoiesis is proposed.

Acetylglucosamine↗
PubMed1980

Metabolic changes in the serum of partially hepatectomized rats.

The factor(s) which trigger the proliferation of liver cells after partial hepatectomy in rats are not yet known with certainty. A series of specific and/or non-specific substances are said to effect the proliferation of liver cells, but metabolic changes after partial hepatectomy possibly also act as a contributory causative factor in the stimulation of those substances. In order to define this question more clearly the changes in the serum concentrations of the following metabolites in the course of time after partial hepatectomy were determined: free fatty acids, cholesterol, triglycerides, glucose, free amino acids and total protein. A reduction in the serum concentrations of some fatty acids and of glucose, and an increase in that of the free amino acids, was observed shortly after partial hepatectomy. The increase in the plasma concentration of immunoreactive glucagon after partial hepatectomy is thus explained and appears not to be of decisive significance for the stimulation of liver cell proliferation after partial hepatectomy.

Amino Acids↗
PubMed1980

Biosynthesis of plasma proteins in serum-free medium by primary monolayer culture of rat hepatocytes.

Morphologically intact rat hepatocytes separated by collagenase perfusion were cultured in L-15/fetal calf serum medium to form a monolayer. Thereafter the hepatocytes were grown in serum-free L-15 medium in which they produced and continuously released plasma proteins. The secreted plasma proteins were collected, separated and characterized by crossed immunoelectrophoresis. Most of the newly biosynthesized plasma proteins secreted into the medium during incubation for thirty hours had the same electrophoretic mobility, antigenicity and staining characteristics as their counterparts in rat serum. The addition of tritium labelled amino acid mixture to the culture medium revealed that the release of radioactively labelled plasma proteins into the culture medium was essentially linear during the thirty hour incubation period. However, saturation of the intracellular pool took place after ca. ten hours of incubation. Addition of leukocytic endogenous mediator, LEM, to cultures of rat hepatocytes caused a profound increase in the relative concentration of acute-phase proteins secreted into the culture medium.

Amino Acids↗
PubMed1980

The effect of fibrinopeptides A and B on experimental allergic encephalomyelitis.

Experimental allergic encephalomyelitis (EAE) was induced in guinea-pigs and rats by sensitization with encephalitogenic antigens and adjuvant. Treatment of the experimental animals by daily intraperitoneal injections with human fibrinopeptides A and B proved to produce significant changes in the course of EAE. In the treated animals as compared to controls the clinical neurological signs of the disease wuch as the number, the severity and the duration of pareses were diminished. Furthermore, the inflammatory alterations of vasopermeability associated with extravasation of plasma proteins and oedema of the neuroparenchyma were significantly less pronounced in the fibrinopeptide-treated animals. Finally, a significantly higher titre of circulating immune complexes was observed in the serum of these animals. However, the treatment with fibrinopeptides A and B did not alter the specific immune response to the antigenic challenge. No differences in anti-basic protein and anti-brain antibody production were observed. The characteristic cellular infiltrates of EAE also showed no significant qualitative or quantitative differences between fibrinopeptide-treated animals and the saline-treated controls. These results suggest that fibrinopeptides A and B act primarily on the immunologically non-specific phase of EAE development by reducing the severity of vascular permeability alterations.

Animals↗
PubMed1980

Red blood cell aggregation in men with coronary artery disease.

The red blood cell aggregation value (AW) in a group of 39 patients with coronary artery disease (CAD) was measured and compared with that of an age- and sex-equivalent group of normals and a group of 12 patients with suspected CAD, whose coronary arteries were found to be normal by coronary arteriography. The AW was significantly higher in CAD patients than in both normal groups (2P < 0.01 and 2P < 0.02 respectively). The AW in a group of 12 CAD-patients treated with the aggregation lowering drug clofibrate was significantly lower than in the untreated CAD-group. The predictive value of AW was calculated by means of mathematical methods. Measurement of AW could be a useful aid in the study of detection of CAD.

Adult↗
PubMed1979

Biological and clinical relevance of human macrophage migration inhibitory factor (MIF).

The first isolation and characterization of a lymphokine, the human MIF (MSF), and the availability of a specific MIF (MSF)-antibody provide an opportunity to establish a new parameter for detection of cellular immune mechanisms in various clinical situations. The precise estimation of quantitative amounts of MIF by sensitive immunochemical techniques could gain new insights into the pathogenesis of a number of clinical disorders. In addition, the exact detection of quantitative amounts of MIF in body fluids could have prognostic value.

Antibodies↗