Search PubMed⌕ Search

Biomedical subjects

G Roos

Publications and source records attributed to G Roos.

At least 181 records · Page 10Linked to original sources
PubMed1986

Sensitivity of human hematopoietic cell lines to an alkyl-lysophospholipid-derivative.

A panel of 16 human hematopoietic cell lines was tested for sensitivity to racemic 1-0-octadecyl-2-0-methyl-glycero-3-phosphocholine (ALP). Effects on proliferation rate, cell cycle distribution, viability and morphology were examined. Cell growth was heavily retarded for all lines at an ALP concentration of 10 micrograms ml-1, which induced profound cell death. 1 microgram ml-1 of ALP was a suitable concentration to make comparisons between the different cell lines. In sensitive lines cell growth was retarded due to induction of multinucleated cells and cytotoxicity. The multinucleated cells could proceed through the cell cycle but cell division was inhibited. The most ALP sensitive cell lines (MN-60, P3Hr-1, Um-42 and RPMI 8226) were B-cell lines, whereas the most resistant lines (U-61M, K562-4 and CCRF-CEM) were of different cell origin. In conclusion, a variable response to ALP was found in 16 human hematopoietic cell lines. As a rule sensitivity to ALP was demonstrated as increased cell death and induction of cells with less than or equal to 2 nuclei, leading to a decreased cell number as compared to control cells.

Cell Cycle↗
PubMed1986

Prognostic value of deoxyribonucleic acid content in metastatic renal cell carcinoma.

The deoxyribonucleic acid content of tumor specimens from 23 patients with metastatic renal cell carcinoma was analyzed prospectively by flow cytometry and static cytophotometry. Of the primary tumors 10 (43 per cent) were homogeneously diploid or near diploid in 8 samples studied from each tumor and 13 (57 per cent) had an aneuploid deoxyribonucleic acid content in 1 to 8 samples. At the end of followup 9 of 10 patients with diploid or near diploid primary tumors were alive, compared to only 1 of 13 with aneuploid primary tumors. Patients with homogeneously diploid or near diploid tumors survived significantly longer compared to those with aneuploid tumor deoxyribonucleic acid content (p less than 0.001). After excision of solitary diploid or near diploid metastases 4 patients had no evidence of disease. In 3 of these patients the primary tumors were diploid or near diploid, whereas 1 had 1 aneuploid and 7 diploid or near diploid samples in the primary tumor. In 10 other patients 28 metastases revealed concordance in deoxyribonucleic acid content with the primary tumors. Our results indicate that deoxyribonucleic acid content might be a useful prognostic discriminator with implications for the clinical management of patients with metastatic renal cell carcinoma.

Adult↗
PubMed1986

Mast cells and lysozyme positive macrophages in bronchoalveolar lavage from patients with sarcoidosis. Valuable prognostic and activity marking parameters of disease?

In the deteriorating group of sarcoidosis patients, progress towards pulmonary fibrosis is a major problem. In order to benefit from corticosteroids, it is important for the treatment to start early. We studied a group of 45 patients with sarcoidosis. Most of them were newly detected patients and none were under or had currently received corticosteroid therapy. The patients were followed for at least six months. We found that increased amounts of polymorphonuclear neutrophils (PMN) or lysozyme-positive macrophages (Lys+MF) and mast cells (MC) in bronchoalveolar lavage (BAL) could implicate a bad prognosis.

Bronchi↗
PubMed1986

Optimum split-half reliabilities for the Rorschach: projective techniques are more reliable than we think.

A new technique for optimizing split-half reliability estimates yielded substantial and comparable increases in indexes of internal reliability for some major Rorschach variables across two diverse samples. Practical suggestions for applying this method to other projective tests were advanced. The inadvisability of computing odd-even reliability coefficients without regard to split-half distributional anomalies is addressed.

Adult↗
PubMed1986

DNA content in renal cell carcinoma. A comparison between flow and static cytometric methods.

Different flow and static cytometric techniques for analysis of DNA content were compared using imprints, fresh and paraffin-embedded tissues from renal cell carcinomas. All methods discriminated between diploid-/near diploid and aneuploid samples and gave comparable results concerning modal ploidy levels (r = 0.744-0.970). For retrospective investigations of renal cell carcinoma, flow cytometry using paraffin-embedded tissues seemed to be the method of choice in determination of DNA content.

Carcinoma, Renal Cell↗
PubMed1985

DNA content in renal cell carcinoma with reference to tumor heterogeneity.

DNA content was successfully determined by flow cytometry in 196 tissue samples from 25 renal cell carcinomas. Twelve tumors (48%) were homogeneously diploid/near-diploid, whereas 11 tumors were aneuploid and 2 tumors were polyploid. Cell clones with different DNA content were found in 11 tumors demonstrating a considerable heterogeneity in the non-diploid tumors; 9 of these 11 heterogeneous tumors contained both aneuploid and diploid cell clones. Tumor samples morphologically classified as grade 1 and 2 were 98% diploid and grade 4 samples were 78% aneuploid. No correlation between DNA distribution and morphologic grade was found for grade 3 tumor samples. Tumor proliferation rate, as determined by the fraction of cells in S-phase, was significantly higher in aneuploid samples compared to normal kidney tissue samples and diploid tumor samples.

Adult↗
PubMed1985

T-cell subsets in multiple myeloma. Impact of cytostatic treatment.

Blood leukocyte and lymphocyte counts, absolute and relative numbers of T-lymphocytes and T-cell subsets were studied in 45 patients with multiple myeloma and 18 healthy controls. No differences were found between untreated patients and controls. In the group of untreated patients similar results were obtained in patients with low-intermediate tumour cell mass as in patients with large tumour cell mass. In patients with large tumour cell mass studied during cytostatic therapy, leukocyte and lymphocyte counts, T-cells, OKT4+ cells and OKT4/OKT8 ratios were significantly lower than in untreated patients. Patients studied at varying intervals (2-28 mo.) after cessation of therapy still exhibited abnormal leukocyte and lymphocyte counts, numbers of T-cells, OKT4+ cells and OKT4/OKT8 ratios.

Aged↗
PubMed1985

Maximizing split-half reliability estimates for projective techniques.

Hand Test data from comparable sets of clinical samples were analyzed for reliability using the usual odd-even split and then also calculating all possible (126) split-half combinations. Maximum stable reliabilities were obtained which were substantially superior to the odd-even method. It was concluded that projective tests are probably more internally reliable than has heretofore been reported.

Adaptation, Psychological↗
PubMed1984

Monoclonal B cells in peripheral blood in non-Hodgkin's lymphoma. Correlation with clinical features and DNA content.

Peripheral blood from 69 patients with non-Hodgkin's lymphoma was examined with respect to B and T cell markers. Evidence for monoclonal B cell was found in 29 cases, 8 of 'high grade' and 21 of 'low grade' malignancy according to the Kiel classification. 17 out of the 29 patients had a normal lymphocyte count. Using conventional staging methods 4 cases of the 29 were in stages II and III, all others in stage IV. The proportion of S-phase cells in peripheral blood, determined by flow cytometry, was found to be elevated in cases with a monoclonal cell population. It is concluded that surface marker analysis of blood cells may be valuable as a diagnostic tool, as an indicator of prognosis and perhaps for the staging procedure of malignant lymphomas.

B-Lymphocytes↗
PubMed1984

Altered distribution of T lymphocyte subsets in lithium-treated patients.

37 lithium-treated patients, 6 prelithium patients and 12 healthy volunteers were studied regarding relative and absolute numbers of peripheral blood lymphocytes, T lymphocytes and T lymphocyte subsets defined with monoclonal antibodies (OKT4, OKT8). Prelithium patients did not differ significantly from healthy volunteers. The relative and absolute numbers of OKT4 cells were lower in lithium-treated patients than in the controls. The relative number of OKT8 cells increased with increasing duration of lithium therapy. Abnormally high as well as low OKT4/OKT8 ratios were found in lithium-treated patients. OKT4/OKT8 ratios were negatively correlated to the duration of lithium therapy. The urinary concentration capacity was significantly lower in patients with low OKT4/OKT8 ratio than in the other lithium-treated patients. It is concluded that lithium in therapeutic doses may have immunomodulatory effects.

Adult↗
PubMed1984

Radiation resistance and the CuZn superoxide dismutase, Mn superoxide dismutase, catalase, and glutathione peroxidase activities of seven human cell lines.

CuZn superoxide dismutase, Mn superoxide dismutase, catalase, and glutathione peroxidase form the primary enzymic defense against toxic oxygen reduction metabolites in cells. To test the importance of these protective enzymes in the cellular radiation response, the enzymic activities of seven different human cell lines were determined in parallel with their clonogenic survival characteristics. A positive correlation between the content of glutathione peroxidase in cell lines and their extrapolation numbers (n) and quasithreshold doses (Dq) was detected. Between the cellular contents of the other enzymes and D0, n, and Dq no positive correlations could be established. An interesting finding was a very high Mn superoxide dismutase content in a malignant mesothelioma cell line P7, which had an extremely high D0, 5.0 Gy.

Catalase↗
PubMed1984

Interferon-induced cell cycle changes in human hematopoietic cell lines and fresh leukemic cells.

A panel of 26 human hematopoietic cell lines was tested for sensitivity to growth inhibition towards interferon-alpha (IFN-alpha) by estimating the effects on cell cycle phase distribution using flow cytometry analysis. The proportion of proliferating cells was assessed by studying the fractional increase of cells in mitosis during a 24-hr vinblastine block. Of 26 cell lines tested, 17 were sensitive to IFN-alpha, and the main cell cycle effect was accumulation in the G0-G1 phase. One Burkitt's lymphoma line, Namalwa, showed a decreased rate of progress through S without any G0/G1 accumulation. Three of the cell lines were also tested with IFN-beta and with IFN-alpha 2 produced by recombinant DNA technology. The latter IFN did not affect one of the cell lines; otherwise, the results were similar to those of IFN-alpha. Six of 16 clinical specimens from patients with hematopoietic neoplasias were IFN-sensitive, all displaying a G0-G1 block. Our results indicate that IFN sensitivity is an individually linked property unrelated to cell origin.

Cell Cycle↗
PubMed1983

Patient with B-cell neoplasia (immunoblastic sarcoma) and the Philadelphia chromosome.

Philadelphia chromosome-positive cells with a standard translocation (9;22) were found in bone marrow and peripheral blood samples from a patient with non-Hodgkin's lymphoma (immunoblastic sarcoma) in the final leukemic phase. The neoplastic clone was of monoclonal B-cell character with surface Ig (mu, kappa) and mouse red blood cell receptors. This is the first case with surface Ig and t(9;22) cells reported without evidence of chronic myeloid leukemia. Also, additional consistent chromosome aberrations were found.

B-Lymphocytes↗
PubMed1982

Establishment and characterization of a human EBV-negative B cell line (MN 60).

A permanent cell line, MN 60, was established from the peripheral blood of a patient with an acute lymphoblastic leukemia (ALL) classified morphologically as being of the L3 type. Cell growth started rapidly in vitro and no feeder cells were needed. Cells of the MN-60 line were identical to the original leukemic cells with respect to surface immunoglobulin (Ig) expression and karyotype, including the presence of four marker chromosomes [1q+, 6q-, t(8;14)]. Continuous proliferation was maintained in stationary suspension culture with a doubling time of 25 h. The cells were tumorigenic in athymic nude mice and had the capacity to form colonies in semi-solid medium in vitro. Monoclonal surface Ig (mu lambda) was demonstrated whereas no cytoplasmic immunoglobulin could be demonstrated. The MN-60 cells were Epstein-Barr virus (EBV) negative as evidenced by EBNA tests and by nucleic acid hybridization studies. The cells expressed HLA-A-C, HLA-DR. beta 2-Microglobulin and cALL, but not Fc gamma. C3, sheep and mouse red blood cell receptors. No reactivity was found with anti-glycophorin A or the anti-BL 38.13 monoclonal antibody. Cell growth was retarded in the G0/G1 phase of the cell cycle after incubation with leukocyte interferon, hydrocortisone, phorbol myristate acetate and dimethyl sulphoxide.

Adult↗
PubMed1982

Melphalan-related leukemia in multiple myeloma.

Five patients with multiple myeloma ending in acute leukemia are described. The preleukemic phase was characterized by anemia, leukopenia and/or thrombocytopenia. The incidence of acute leukemia in myeloma was calculated to be 6%. Melphalan therapy for more than two years increased the incidence to 14%. All patients who developed leukemia had received a total melphalan dose of at least 1 100 mg.

Acute Disease↗
PubMed1982

Differentiation associated changes in isozyme expression of amino acid naphthylamidase in myeloid cells.

Triton X-100 resistant isozymes of amino acid naphthylamidase were studied by polyacrylamide gel electrophoresis, gel filtration and affinity chromatography in samples from 12 human haematopoietic cell lines, 8 leukaemic patients and 4 healthy persons. Affinity to WGA-Sepharose seemed to be correlated to differentiation in the myeloid series, indicating differences in the N-acetyl-glucose-amine content of the enzyme molecules in mature and immature cells. A high carbohydrate content might be characteristic for mature myeloid cells. By trypsin digestion of intact cells and analysis of released enzyme activities into the medium new information on isozyme expression of amino acid naphthylamidase in the granulocyte-monocyte lineages could be achieved.

Aminopeptidases↗