Search PubMed⌕ Search

Biomedical subjects

G Romeo

Publications and source records attributed to G Romeo.

At least 307 records · Page 17Linked to original sources
PubMed1982

Genetic and demographic characterization of a population with high incidence of fucosidosis.

A random sample of unrelated students living in Grotteria and Mammola, two villages of Southern Italy from where most of the Italian patients affected with fucosidosis originate, was analyzed for seven red cell enzyme polymorphisms and seven blood group polymorphisms. The observed gene frequencies are not significantly different from those typical of Southern Italy. Demographic data on the same sample were also collected and indicate isolation and high inbreeding in these villages.

Adolescent↗
PubMed1981

[Transport of cytidinediphosphocholine in different biological models. I - Synthesis and characterization of (5 3H, Met 14 C) cytidinediphosphocholine].

In order to investigate the transport of CDP-choline across the biological membranes we have synthesized the double labeled CDP-choline. The (5 3H, methyl 14C) CDP-choline is synthesized by a crossed condensation of (5 3H) CMP and methyl 14C) p-choline using DCC as condensing agent. The product of the reaction is identified by chromatographics and spectroscopics methods.

Carbon Radioisotopes↗
PubMed1981

[Transport of cytidinediphosphocholine in different biological models. II - Transport of (5 3H, Met 14C) cytidinediphosphocholine in isolated and perfused rat liver].

The uptake of double labeled CDP-choline by isolated and perfused rat liver has been investigated. The time course of CDP-choline uptake is different for 3H and 14C, showing the existence of the degradation process of the molecule. The identification and characterization of CDP-choline and its metabolites, in the perfusate and in the hepatic extract are performed by high pressure liquid chromatography.

Animals↗
PubMed1981

Ultrastructural study of parietal cells before and after parietal cell vagotomy in patients with duodenal ulcer.

The records of 16 patients of a total of 402 who underwent parietal cell vagotomy without a drainage procedure for duodenal ulcer were reviewed. Biopsies of gastric mucosa were taken before the operation and six months, two years and three years postoperatively. Ultrastructural studies using electron microscopy detail the modifications of the parietal cells which are the main target of the vagal denervation procedure. The parietal cells are greatly modified within six months after parietal cell vagotomy with a significant reduction of the secretory surface of the cells and a sharp increase of tubulovesicular formations regain their preoperative morphologic appearance after two to three years but not their secretory capacity.

Adult↗
PubMed1980

Cystic fibrosis alpha 2-macroglobulin protease interaction in vitro.

alpha 2-Macroglobulin was purified from plasma of five cystic fibrosis patients and five normal controls. SDS gel electrophoresis of native alpha 2-macroglobulin from cystic fibrosis patients and normal donors showed identical subunit molecular weights, as did trypsin cleavage products. Cystic fibrosis and control alpha 2-macroglobulins were indistinguishable by isoelectric focusing and exhibited appropriate shifts in isoelectric point following binding of trypsin. The trypsin-binding capacities of control and cystic fibrosis alpha 2-macroglobulins did not differ, nor did the esterolytic activity of the trypsin-alpha 2-macroglobulin complexes.

Adolescent↗
PubMed1980

Regulation of cyclic AMP levels in human lymphocytes and lymphoblasts by prostaglandins.

The sensitivity of human lymphocytes to prostaglandins was compared to that of two lines of cultured lymphoblasts. It was found that 25 nM PGE1 increased lymphocyte cyclic AMP levels twofold, but had no effect on lymphoblasts. At 2.8 micrometers PGE1 the increase of cyclic AMP in lymphocytes was 14 fold versus a 1-3 fold increase in lymphoblasts. Lymphoblasts maintained a response to 6-keto-PGE1, isoproterenol and histamine similar to that observed in lymphocytes. Thus, it appears that the rapidly proliferating lymphoblasts excape signals inhibiting proliferation by impairing the function of the PGE receptor.

Alprostadil↗
PubMed1980

Antibodies cross-reacting with porcine and human trypsin in cystic fibrosis.

A solid-phase radioimmunoassay has been developed for the titration of TbIgG (trypsin binding IgG) in sera of CF patients. Using this assay system it has been found that TbIgG are primarily directed against porcine trypsin and cross-react with human trypsin. A specific subpopulation of anti-human trypsin antibodies has not been found, making unlikely the occurrence of an autoimmune process in CF, at least against human pancreatic enzymes.

Animals↗
PubMed1980

Competition for trypsin binding between alpha 2-macroglobulin and antitrypsin antibodies in cystic fibrosis.

As previously reported, Tblg, especially TblgG, have been identified in CF patients, and their production appears to be elicited by the porcine trypsin ingested daily by most CF patients. We have measured by IP assay the binding of 125I-trypsin to a alpha 2M and TblgG in plasma of CF patients and controls. Our results do not confirm previous reports of decreased trypsin binding to alpha 2M in whole CF plasma with respect to controls. We have shown, however, that antitrypsin antibodies from CF patients or from rabbits immunized against trypsin compete with alpha 2M for binding with trypsin. This competition can cause apparent decrease of trypsin binding to alpha 2M in vitro.

Antibodies↗
PubMed1979

Preparation and analysis of new sulfonium derivatives from S-adenosyl(5')-3-methylthiopropylamine.

The paper reports the preparation and the chromatographic separation of new deaminated analogs of S-adenosyl(5')-3-methylthiopropylamine, i.e. S-adenosyl(5')-3-methylthiopropanol and S-inosyl(5')(-3-methylthiopropanol. These compounds can be used as specific inhibitors of polyamine biosynthesis. It is also reported the characterization of new sulfonium compounds by U.V. spectrophotometry, thin layer chromatography, high voltage electrophoresis, hydrolysis to known fragments.

Onium Compounds↗
PubMed1979

Detection of carriers and prenatal diagnosis for fucosidosis in Calabria.

A significant proportion of patients affected with fucosidosis have Italian ancestors. We assayed for alpha-fucosidase activity purified mononuclear cells and/or leukocytes obtained from 64 members of two large pedigrees from Calabria, in which seven children had been diagnosed as affected with fucosidosis. Of these 64 individuals, 22 were diagnosed as carriers, while the values for the remainder were within normal limits, indicating a clear bimodal distribution among individuals at risk. These data confirm that carrier detection for fucosidosis requires the measurement of alpha-L-fucosidase activity in purified mononuclear cells from peripheral blood. In addition, this program has made prenatal identification of an affected fetus possible.

Amniotic Fluid↗
PubMed1979

Non-inactivation of an x-chromosome locus in man.

Cloned fibroblasts from women heterozygous for X-linked ichthyosis (steroid sulfatase deficiency) were examined to see whether or not this locus is subject to X-inactivation. Of 103 clones examined, all had normal levels of steroid sulfatase activity. Two of the women studied were also heterozygous for glucose-6-phosphate dehydrogenase deficiency. This allowed the demonstration that both X chromosomes were represented as the active X in various clones and that selection did not account for these findings. Thus, the steroid sulfatase locus, like the Xga locus to which it is linked, appears to escape X-inactivation in man.

Dehydroepiandrosterone↗
PubMed1979

Relationship between alpha-L-fucosidase deficiency in plasma and alpha-L-fucosidase activity in leukocytes.

After testing a population sample of 185 hospitalized Italian children for the plasma alpha-L-fucosidase deficiency and establishing an approximate threshold value between heterozygotes and wild-type homozygotes, we analyzed by two statistical methods the distribution of the two genotypes. The results obtained by probit analysis agree with threshold and average values expected on the basis of the Hardy-Weinberg equilibrium. In addition, the level of alpha-fucosidase in leukocytes of 12 individuals with deficiency of alpha-fucosidase in plasma was found to be significantly lower than that of 61 controls (P less than 0.005). These results indicate that the mutation(s) causing a deficiency of alpha-fucosidase in plasma is (are) also expressed in leukocytes.

Carbohydrate Metabolism, Inborn Errors↗