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Biomedical subjects

G Robertson

Publications and source records attributed to G Robertson.

At least 19 recordsLinked to original sources
PubMed1992

The attachments of chromatin loops to the nucleoskeleton.

It is widely assumed by cell biologists that chromatin is looped by attachment to some nuclear skeleton. 'Structural' attachments might be mediated through specific sequences; these would be attached in most cells in an organism, underlying the basic structure of the mitotic chromosome and persisting throughout interphase. 'Functional' attachments might also exist, perhaps if active polymerases are attached to the skeleton and replication and transcription occur as DNA is reeled through them. Cells of different tissues--and even cells of the same tissue--would have different attachments of this type. Problems associated with demonstrating these two kinds of attachment are discussed. We find little good evidence for 'structural' attachments and explore the idea that 'functional' attachments are the only kind that exist: 'functional' attachments involving active transcription units might be stable enough to organize chromatin during both interphase and mitosis, but 'dynamic' enough to allow duplication of attached sequences without disrupting loops.

Animals↗
PubMed1992

Subdiaphragmatic vagotomy in rats induces systemic hypertension and sodium retention.

We examined the acute effects of bilateral subdiaphragmatic vagotomy (BSV) on blood pressure and renal function in female Sprague-Dawley rats. Mean arterial pressure was greater (p < 0.0001) in rats with BSV than in sham-operated rats (SOR). Rats with BSV had a significantly lower effective renal plasma flow (p < 0.01), total sodium excretion (p < 0.005), fractional sodium excretion (p < 0.01), urine flow (p < 0.01), and fractional excretion of water (p < 0.02) than SOR. The glomerular filtration rate was not significantly different between the 2 groups of rats. Plasma potassium was greater in rats with BSV than in SOR (p < 0.02). Pretreatment with an inhibitor of the angiotensin-converting enzyme prevented the above changes in rats with BSV. Changes in renal function and mean arterial pressure could not be attributed to antidiuretic hormone since plasma levels of antidiuretic hormone were lower in rats with BSV than in SOR (p < 0.002). In addition, the activity of the sympathetic system was decreased in rats with BSV, as suggested by the lower plasma levels of epinephrine (p < 0.003) and norepinephrine (p < 0.02) and the significantly lower renal tissue concentrations of norepinephrine (p < 0.03). No significant changes in renal tissue concentrations of acetylcholine or choline, its precursor, were observed in BSV rats when compared to SOR, suggesting a lack of renal parasympathetic innervation. Plasma renin activity was lower in rats with BSV (p < 0.02) than in SOR, but this effect was blunted in rats given an angiotensin-converting enzyme inhibitor prior to BSV.(ABSTRACT TRUNCATED AT 250 WORDS)

Aldosterone↗
PubMed1991

Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene.

A homologue of the herpes simplex virus (HSV) glycoprotein D gene has been identified in the genome of equine herpesvirus-1 (EHV-1, equine abortion virus). An open reading frame in the middle of the short unique (US) region is capable of encoding a polypeptide of 402 amino acids that has 26% and 20% of its residues matching pseudorabies virus (PRV) gp50 and HSV-1 gD, respectively. Despite this low level of similarity, the positional identity of six cysteine residues and certain motifs, and the location of the EHV-1 gene, clearly define the EHV-1 polypeptide as one of a family of "gD-like" proteins. Two transcripts of 3.3-3.6 kb and 5.4-5.9 kb were identified, consistent with coterminal mRNAs for the EHV-1 gD gene and the adjacent upstream gene, respectively. Partial sequencing of other regions in US also revealed EHV-1 homologues of HSV-1 gE and gI genes, and a possible equivalent gene to PRV gX. By analogy with the ability of HSV-1 gD and PRV gp50 to induce strong anti-viral immune responses, the EHV-1 gD gene product is expected to be an excellent candidate for development as a vaccine antigen.

Amino Acid Sequence↗
PubMed1991

Role of vasopressin in rats with bilateral ureteral obstruction.

After unilateral release of bilateral ureteral obstruction (BUO), there is a significant increase in renal vasoconstriction that accounts for the marked decrease in glomerular filtration rate and effective renal plasma flow seen in this setting. We examined the potential role of antidiuretic hormone (ADH), a vasoconstrictor of the renal circulation, on renal hemodynamics in female Sprague-Dawley rats with BUO of 24-hr duration. Rats with BUO had significantly higher plasma values of ADH 65.1 +/- 12.2 vs. 12.1 +/- 4.1 pg/ml), sodium (145.4 +/- 0.91 vs 138.6 +/- 1.06 mEq/liter), and osmolality (375.6 +/- 2.0 vs 310.1 +/- 3.6 mOsm/kg) than sham-operated rats. Rats with BUO pretreated with enalapril, an angiotensin-converting enzyme inhibitor, before obstruction had somewhat higher, but not significantly different, plasma values for ADH (84.6 +/- 20.8 pg/ml) than rats with BUO not given enalapril. Rats with unilateral ureteral obstruction of 24-hr duration had plasma levels of ADH (8.2 +/- 1.3) not different from those in sham-operated rats. Rats with BUO pretreated with a specific antagonist of the V1-type receptor for ADH had significantly greater values for the glomerular filtration rate (2.31 +/- 0.24 vs 1.44 +/- 0.08 ml/min/kg body wt) and the effective renal plasma flow (8.95 +/- 0.71 vs 3.81 +/- 0.44 ml/min/kg body wt) and significantly lower values for mean arterial pressure (140.3 +/- 2.0 vs 159.1 +/- 5.5 mm Hg) than did BUO rats not given the antagonist. The results indicate that high levels of ADH play an important role in the decrease in the glomerular filtration rate and effective renal plasma flow observed in rats with BUO of 24 hr. The significant increase in ADH levels after BUO of 24-hr duration may be due to an increase in osmotic stimulation as a consequence of hypernatremia. Activation of the renin-angiotensin axis, known to occur after BUO or unilateral ureteral obstruction of 24-hr duration, does not appear to have a role in the increased circulating levels of ADH.

Animals↗
PubMed1990

A health promotion module for undergraduate medical students.

This paper describes a 4-day module on health promotion which is part of the undergraduate programme for medical students at the University of Edinburgh. Early experience of the module from both the learner and teacher perspectives are reported. The module is part of a new 4-week course on community medicine for fourth- and fifth-year students and is a collaborative venture between people working in the field of health promotion in the University, the local health service and the national health education organization, the Scottish Health Education Group. The aims of the module, together with details about the content, teaching methods and form of assessment are described. Some of the formal evaluation results are presented. The module has proved highly popular with both students and teachers. The paper concludes with a discussion of the key factors that have been important in the successful development of the module.

Attitude of Health Personnel↗
PubMed1990

Correlates of crime among women offenders.

In 1967, Professor Trevor Gibbens interviewed 245 women admitted to Holloway Prison in London. The criminal records of these women to the end of 1977 were examined, at which time the mean age of the group was 39.6. Data from these records were correlated with the information obtained by interview in 1967. Alcohol abuse (but not drug taking) was associated with both seriousness and frequency of offending, as was self-reported depression outside of prison. Neither variable can be viewed as being independent of the criminal record data but a disproportionate number of women who were in the highest crime subgroup reported unhappy and disrupted early home circumstances and this variable also had predictive power in relation to those first convicted in 1967. However, though statistically associated with the measure of criminality used in the study, no variable was able to account for more than a small part of the relevant variance. The most marked feature of the study was the heterogeneity of this highly selected population.

Adult↗
PubMed1989

An evaluation of semen analysis and in-vitro tests of sperm function in the prediction of the outcome of intrauterine AIH.

Twenty-nine couples with an average of 5 years of infertility were selected for treatment by intrauterine insemination of washed semen (AIH). The criteria for selection were (i) the female partner showed no detectable fertility disorders by routine screening; (ii) the male partner showed subnormal semen quality on conventional semen analysis. Ovulation was stimulated uniformly with clomiphene citrate and precipitated with human chorionic gonadotrophin (HCG). Inseminations were performed 31-32 h post-HCG, with the day of HCG determined by ultrasound monitoring of follicular development. The fertilizing capacity of the male partners' spermatozoa was tested in vitro using donated human oocytes and/or the zona-free hamster oocyte penetration assay. Up to eight cycles of AIH were alternated with cycles of natural intercourse. While no pregnancies occurred in the group during normal coital cycles, the AIH pregnancy rate was 17% per couple, but only 3% per insemination cycle. Four further pregnancies were achieved spontaneously in couples from the study group within 3 years of completion of the AIH therapy and four patients became pregnant following subsequent GIFT or IVF treatments. Neither of the in-vitro tests was helpful in predicting the outcome of AIH, spontaneous pregnancy nor of subsequent assisted conception procedures.

Adult↗
PubMed1989

Treatment for offender patients: how should success be measured?

The criteria used to judge the success of treatment for mentally ill (psychotic) patients are the same for offenders and non-offenders. For those offender patients detained as psychopaths, the incremental validity of the medical concept is dubious and recourse is often made to the use of the criminal criterion of reconviction in order to measure therapeutic effectiveness. One such study is reported here and others are reviewed. It is argued that reconviction is not a valid measure of psychiatric treatment and that the criteria used to judge therapeutic effectiveness in forensic psychiatry should not differ from those used in all other forms of psychiatric care.

England↗
PubMed1988

Arrest patterns among mentally disordered offenders.

One unknown factor in the link between crime and mental illness is whether or not mentally ill offenders are more liable than others to arrest. Ninety-one mentally ill, and 76 normal, criminally offending men were asked about the circumstances of their offence and arrest. A majority of mentally ill men had been arrested at the scene of the crime, and more than a quarter of the schizophrenic group had reported themselves to the police. We argue that when many offenders are either unreported or undetected, the increased vulnerability of the mentally ill to detection and arrest makes them disproportionately liable to detection. Attempting to assess the relationship between crime and mental illness is thus extremely difficult.

Crime↗
PubMed1987

Mentally abnormal offenders: manner of death.

A 23 year follow up of deaths in a population of mentally disordered patients was carried out, and a typical case history is reported. A quarter (71) of the deaths reported were unnatural, verdicts of suicide or accidental death or open verdicts having been recorded. For men in most age groups the proportion of deaths by suicide was two to three times greater than in the general population in 1982; the rate among those aged 25-29 was five times that in the general population. Differences in the rate of unnatural death among diagnostic categories of mental illness were not significant, but the proportion of unnatural deaths among the mentally handicapped will probably eventually be lower than that among psychotic offenders or those with personality disorders. Violent death occurs at an older age in those with affective disorders. Social isolation and alienation add to the handicap of mental disorder in this group of people, and these sometimes difficult but always vulnerable patients must continue to be offered asylum other than prison.

Adult↗
PubMed1987

Detention in Broadmoor. Factors in length of stay.

When the length of stay of restricted patients admitted to Broadmoor under the legal category of psychopathic disorder was examined, the factor found to be of primary importance was the gravity of the admission offence. If the men's offences did not cause personal injury, they had a good chance of early release. Patients convicted of violent or sexual offences, and particularly those who attacked strangers, made up the great majority of the long-term group. For the mentally ill there was no relationship between length of stay and admission offence: instead the data suggested that severity and chronicity of illness were the main relevant factors.

Antisocial Personality Disorder↗