Search PubMed⌕ Search

Biomedical subjects

G Renoux

Publications and source records attributed to G Renoux.

120 records · Page 7Linked to original sources
PubMedDate not supplied

[Passive hemagglutination test for individual or epidemiologic diagnosis of human brucellosis].

Coupling of an extract from B. abortus strain 99 to sheep red blood cells by chromium chloride allowed to develop a highly specific and reliable passive hemagglutination test (H). Indeed, H was positive in 99.8 per cent sera from 968 known Brucella-infected heifers, while tube agglutination revealed only 59 per cent and complement fixation 90.9 per cent positive cases. H was positive in 153 sera from 778 proven or suspected human brucellosis where agglutination and/or complement fixation tests were positive, and in 21 sera negative to conventional tests. H demonstreated remote or undiagnosed human B. abortus infections in 28.6 per cent of 18 367 human sera randomly examined in a rural area. Therefore, a simple easy-to-perform passive hemagglutination test could replace a battery of conventional tests for the diagnosis of individual brucellosis, and epidemiologic surveys. The present study points to a high prevalence of undiagnosed brucellosis in man, specially in those area where cattle are raised.

Animals↗
PubMedDate not supplied

[Mechanisms of action of levamisole, stimulating cellular immune responses].

Since LMS possesses a thiazole and an imidazole (IMZ) moiety, we compared in vivo activities of LMS and of IMZ on immune responses of mice, IMZ was unable to increase the number of direct or indirect plaque-forming spleen cells to sheep red blood cells (SRBC), the magnitude of delayed hypersensitivity to SRBC, and did not evoke the production of a serum factor which passively transferred the immune stimulation. LMS was repeatedly active in these three tests. In vivo activities of LMS appear to be linked to its sulphur moiety able to recruit and activate a new population of T cells, through serum mediators.

Animals↗
PubMedDate not supplied

[Influence of levamisole on antibody production].

IgM and IgG anti-sheep red blood cells agglutinins were measured from dayy + 2 to day + 30 in SPF mice immunized with 108 SRC and treated with 2.5 or 25 mg LMS/dg. Untreated control mice produced high levels (1:8200) of IgM-antibodies from day + 4 to day + 20. Early, day + 4, IgG-agglutinins appeared together with IgM-antibodies in mice treated with 2.5 mg LMS/kg, whereas administration of 25 mg LMS/kg induced the synthesis of antibodies belonging only to the IgG class. Titres were significantly lower (1:512) in LMS-treated mice than in controls. The switch to IgG of antibody formation is another for the ability of LMS to stimulate T cell activities.

Animals↗