Biomedical subjects

G R van der Hoff

Publications and source records attributed to G R van der Hoff.

4 recordsLinked to original sources

PubMed1999

Trace analysis of pesticides by gas chromatography.

The analysis of pesticides is relevant to both food quality and the environment. Many laboratories are occupied with the analysis of pesticides in food, water or soil. Capillary gas chromatography is the technique most widely used in pesticide analysis. In present laboratory practice it serves as a screening method for over 300 pesticides. In this review we describe the role of gas chromatography as an analytical tool in combination with currently used or recently developed sample preparation techniques.

Chromatography, Gas↗

PubMed1999

Potential of a simple mechanical extraction system for the rapid extraction of pesticides from foodstuffs. Application to the determination of organochlorine pesticides in lettuce.

The efficiency of a simple mechanical extraction system as applied in veterinary drug analysis has been tested in the field of pesticide residue analysis. As a first application, the system was used for the extraction of organochlorine pesticides from vegetables. The convenience of this simple extraction system consists of performing mechanical extraction in disposable polyethylene-based extraction bags, reducing considerably manual operation and cross-contamination. For the tested compounds and matrix (lettuce), recoveries of the 10 organochlorine pesticides performed at two spiking levels (n = 4 each level and compound) ranging between 0.06 and 3.3 mg/kg were between 60 and 80%, with most standard deviations <5%. The extraction method appeared to be simple and fast with a great potential for the analysis of many pesticide-matrix combinations.

Chromatography, Gas↗

PubMed1996

Determination of organochlorine compounds in fatty matrices. Application of rapid off-line normal-phase liquid chromatographic clean-up.

A clean-up method for organochlorine compounds in fatty samples based on normal-phase liquid chromatography (NPLC) is described. To this end, an existing clean-up procedure which uses column switching for the separation of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) from the fat matrix was simplified to a single silica LC column procedure. The use of an LC column packed with 3 microns silica enables complete fat/OCP separation in a total fraction volume of 12 ml, and results in a fully automated clean-up procedure that takes only 32 min per sample. The method showed average recoveries of 80-110% in the concentration range of 1-510 micrograms/kg, with relative standard deviations of less than 10%. Limits of determination were in the range of 0.5-50 micrograms/kg. The simplified approach has shown its potential for a variety of samples, such as milk, pork fat, animal feed and cod liver oil, showing its general applicability to fatty samples.

Adipose Tissue↗

PubMed1996

Automated solid-phase extraction coupled to gas chromatography with electron-capture detection: a combination of extraction and clean-up of pyrethroids in the analysis of surface water.

The combination of automated solid-phase extraction (SPE) and large-volume introduction gas chromatography electron-capture detection (GC-ECD) is used for the determination of synthetic pyrethroids in surface and drinking water. The selectivity that is needed for the use of GC-ECD of environmental samples is achieved by addition of a polarity modifier to the sample. In this study, sufficient methanol was added to 19 ml of sample to obtain a level of 30% of modifier prior to SPE. The cartridge is washed with one additional ml of 30% methanol in water. After drying, the cartridge is eluted with toluene. GC-ECD analysis is performed by on-column introduction of 100 microliters of the toluene extract. The whole procedure was automated by using an automated solid-phase extraction apparatus. Limits of detection (LODs) for the pyrethroids in surface water are in the range of 1 to 8 ng/1 (signal-to-noise ratio = 3); even lower limits of detection are possible for drinking water.

Autoanalysis↗