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G R Svishcheva

Publications and source records attributed to G R Svishcheva.

6 recordsLinked to original sources
PubMed2001

[Radiation mapping of the short arm of swine chromosome 2].

In recent years, maps of mammalian genomes have been acquiring increasingly higher resolution. Integration of maps of different types has become possible. As a tool in integrating maps of mammalian genomes of different types, high-resolution mapping with radiation-induced hybrids (RH) is used. Here, we present an RH6000 map of the short arm of porcine chromosome 2. The map contains 15 microsatellites and five genes (for parathyroid hormone, lactate dehydrogenase A, myogenic factor, follicle-stimulating hormone beta, and calpain I). The RH panel was obtained on the basis of a hybrid cell line bearing the single porcine chromosome 2 against the background of mink chromosomes. The mean frequency of preserving markers examined in the panel was 18.3%. Integration of four genes in the panel and a comparison of gene order in homologous regions of human and porcine chromosomes are presented.

Animals↗
PubMed2000

[Radiation hybrid mapping: statistical criteria and their properties].

Statistical tests specially developed for radiation hybrid (RH) mapping, modified likelihood-ratio test and Akaike's information content, are proposed. They allow several most likely gene orders to be identified to a certain accuracy. The properties of the proposed tests that characterize the accuracy of gene ordering as dependent on the number of genes mapped, the position of the selective gene, and the sample size have been studied. Recommendations as to the practical use of these tests are presented.

DNA Damage↗
PubMed1999

[Statistical methods of radiation hybrid mapping: selective models].

A new type of selective models extending the possibilities of the existing methods of radiation hybrid mapping has been developed. These models are intended for mapping a "combined" group of genes. The genes are detected in a given clone by either direct molecular genetic methods or methods of isozyme analysis, which allow the gene to be detected indirectly, i.e., by the presence of its protein product. This type of selective models allows for both strict and nonstrict retention of the selective gene in the hybrid clone (the strictly and nonstrictly selective media, respectively). Asymptotic properties of the new type of models have been studied. It has been demonstrated that all of them are pseudo-Markovian and additive and may be used for mapping an unrestricted number of genes. The minimum numbers of genes being localized for which different variants of the models hold true have been estimated.

Clone Cells↗
PubMed1999

[Statistical methods of radiation hybrid mapping: analysis of a small number of genes].

A statistical method for radiation hybrid (RH) mapping of a small number of genes detected in clones by means of isozyme analysis is suggested. This method allows analytical solution of the problem of gene ordering and qualitative comparison of relative distances between the genes that are being mapped. The possibilities offered by the suggested method are illustrated using earlier data on the distribution of four isozyme markers in human and vole (Microtus rossiaemeridionalis) clones. These markers are hypoxanthine phosphoribosyl transferase (HPRT), PGK, G6PD, and GALA.

Animals↗
PubMed1993

[Statistical validation of a radiation mapping method].

Correct statistical treatment of results of Goss and Harris experiments on radiation hybrid mapping is proposed. Using stochastic simulation a problem of an optimal sample size is investigated. Reasons for a repetition of experiments with different doses of radiation are discussed.

Animals↗