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Biomedical subjects

G R Stewart

Publications and source records attributed to G R Stewart.

At least 73 records · Page 4Linked to original sources
PubMed1981

Transthoracic right atrial cannulation for total parenteral nutrition- case report.

A man with severe peripheral vascular disease and requiring total parenteral nutrition because of short bowel syndrome was referred because a central venous catheter could not be inserted by conventional techniques. A right thoracotomy was performed and a Hickman catheter inserted via the right atrial appendage into the right atrium. This catheter was used for a total of seven months for total parenteral nutrition. For the last two months of this time, the patient was maintained at home on a Home Parenteral Nutrition Programme. After four months of total parenteral nutrition the patient developed recurrent fevers and the catheter was found to have migrated from the right atrium into the pulmonary artery. The catheter was resited under X-ray control and used for a further three months until the recurrence of fever and dyspnoea heralded the onset of septic pulmonary emboli resulting in his death.

Cardiac Catheterization↗
PubMed1980

Gastric bypass for giant morbid obesity.

A 35-year-old man, grossly incapacitated by giant morbid obesity, and showing pathological sequelae of it, weighed 225.45 kg at presentation. After gastric bypass, his body weight decreased by 48% over approximately one year. After abdominoplasty and reduction of thigh skin folds, his weight stabilized at 116.36 kg. All sequelae of the morbid obesity had resolved, and the patient was able to resume a normal lifestyle and to re-enter the work force.

Adult↗
PubMed1980

A comparison of the amino acid sequences of rabbit skeletal muscle alpha- and beta-tropomyosins.

Elucidation of the complete amino acid sequence of rabbit skeletal beta-tropomyosin has shown that it has the same number of residues (284) per chain as the alpha component from the same source. The presence of more than one form of beta-tropomyosin was indicated by the detection of heterogeneity at 11 positions in the sequence. The ratio of the major form to the minor form(s) was estimated to be about 10:1. Of the 39 amino acid residue differences in the major alpha and beta forms, most involve chemically similar residues with only two leading to a more negative net charge on the beta form (Ser-229 to glutamic acid and His-276 to asparagine). These replacements do not significantly affect the repeating heptapeptide pattern of nonpolar and polar residues in tropomyosin nor the 14-fold periodicity of acidic and outer nonpolar residues implicated in its binding to F-actin. The larger number of substitutions in the COOH-terminal half of the protein is reflected in differences in the smoothed alpha-helix parameters of the beta-tropomyosin sequence when compared with that of the alpha component. These differences may be related to a lower binding affinity of beta-tropomyosin to troponin.

Amino Acid Sequence↗
PubMed1979

Rubber sump drainage of enterocutaneous fistulae.

Effective sump drainage of high enterocutaneous fistulae, together with alimentary rest and total parenteral nutrition, is now an integral part of the modern management of patients with this condition. The low tissue reactivity of the plastic and polymer materials currently used in most drainage tubes appears however, to be counterproductive to the establishment of a discrete fistula track and control of the fistula. A case is made for the use of red rubber sump drains for enterocutaneous fistulae. The greater tissue reactivity of rubber is reviewed and confirmed by animal experimentation. A method of rubber sump drainage of enterocutaneous fistulae developed during the management of 83 such fistulae is described.

Animals↗
PubMed1977

Early detection of breast cancer.

One thousand referred patients with breast symptoms, a history of breast disease, or a family history of breast carcinoma have been examined at a suburban breast clinic. This is a preliminary survey of the data and results so far obtained from the initial examination of these patients. The examination consisted of history-taking by means of a questionnaire, physical examination of the breasts, thermography and xeromammography. A report correlating the findings was forwarded to the referring doctor in each case and any necessary action was indicated in the report. Seventy-eight patients were thought to require biopsy of a suspicious lump or mammographic lesion (7.8% of the total number); 76 patients actually had biopsies, and 18 patients were found to have carcinoma of the breast (23.8% of the total number of patients who had biopsies). The overall incidence of carcinoma in the 1000 women attending the clinic was 1.8% at the initial visit. While general population screening for breast carcinoma may be hard to justify, from both the detection rate and an economic point of view, it would appear from our experience to date that screening of selected high-risk referred patients is justified and, in fact, desirable.

Adenocarcinoma↗
PubMed1973

The isolation and partial characterization of ribonuclease A from Bison bison.

1. Bison ribonuclease was isolated from pancreas glands of Bison bison by acid extraction, (NH(4))(2)SO(4) fractionation, affinity chromatography on Sepharose-5'-(4-aminophenylphosphoryl)uridine 2',3'-phosphate and ion-exchange chromatography on Bio-Rex-70. 2. The selectivity of the affinity column towards bison ribonuclease in heterogeneous protein solutions was greatly improved by employing piperazine buffers at pH5.3, which decreased non-specific interactions of other proteins. Rapid desorption from the affinity column was obtained with sodium phosphate buffer (pH3). 3. Bison ribonuclease has a total amino acid content very similar to ox ribonuclease. Inactivation of bison ribonuclease with iodoacetic acid leads to the formation of 0.62 residues of pi-carboxymethylhistidine and 0.36 residues of tau-carboxymethylhistidine. The amino acid composition of peptides isolated from diagonal peptide ;maps' and also of peptides isolated after pH1.6 and 2.4 two-dimensional high-voltage electrophoresis of a digest of bison ribonuclease labelled with pyridoxal 5-phosphate indicates that there is complete homology between ox and bison ribonucleases. 4. The Schiff-base attachment site of pyridoxal 5-phosphate was identified as lysine-41 by NaBH(4) reduction followed by peptide isolation.

Amino Acid Sequence↗